[1]王金香,蒋明义,马芳芳,等.玉米促分裂原激活蛋白激酶ZmMPK7基因的表达特性及功能分析[J].南京农业大学学报,2011,34(1):68-73.[doi:10.7685/j.issn.1000-2030.2011.01.013]
 WANG Jin-xiang,JIANG Ming-yi,MA Fang-fang,et al.Expression characterization and function analysis of ZmMPK7 from Zea mays seedlings[J].Journal of Nanjing Agricultural University,2011,34(1):68-73.[doi:10.7685/j.issn.1000-2030.2011.01.013]
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玉米促分裂原激活蛋白激酶ZmMPK7基因的表达特性及功能分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年1期
页码:
68-73
栏目:
出版日期:
2011-02-28

文章信息/Info

Title:
Expression characterization and function analysis of ZmMPK7 from Zea mays seedlings
作者:
王金香蒋明义马芳芳丁海东
南京农业大学生命科学学院,江苏,南京,210095
Author(s):
WANG Jin-xiang JIANG Ming-yi MA Fang-fang DING Hai-dong
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
玉米 促分裂原激活蛋白激酶 表达特性 功能分析 非生物胁迫
Keywords:
maize mitogen-activated protein kinase(MAPK) expression characterization function analysis abiotic stress
分类号:
S513
DOI:
10.7685/j.issn.1000-2030.2011.01.013
文献标志码:
A
摘要:
以玉米(Zea mags L.)为材料,利用RT-PCR和RACE技术,从ABA处理的玉米叶片中克隆了1个新的A族促分裂原激活蛋白激酶(MAPK)基因,命名为ZmMPK7(GenBank登录号:EU616650)。生物信息学分析表明:该基因全长1 709 bp,编码397个氨基酸,推测相对分子质量为44.9×103,pI 5.31。ZmMPK7包含有MAP激酶11个相对保守结构域和1个TEY的磷酸化基序。对玉米不同组织中ZmMPK7基因的转录水平分析表明:ZmMPK7基因的转录没有组织专一性。非生物胁迫处理下ZmMPK7基因表达研究发现:ABA、H2O2、干旱、盐胁迫、低温及重金属处理均诱导ZmMPK7基因的转录水平显著上调。原生质瞬时表达载体定位试验表明:ZmMPK7定位于玉米原生质体的细胞质中。上述结果显示:ZmMPK7在植物对环境胁迫响应中具有重要的作用。
Abstract:
A novel group A MAPK(mitogen-activated protein kinase)gene designated ZmMPK7 was isolated and characterized by RT-PCR and RACE from ABA treated maize(Zea mays L. ). Bioinformatics analysis indicated that the full-length cDNA sequence was 1 709 bp and encodes a 397 amino acid protein with a predicted molecular mass of 44.9 ×103 and a pI of 5.31.ZmMPK7 contains all 11 of MAPK conserved subdomains and the phosphorylation motif TEY. Amino acid sequence alignment revealed that ZmMPK7 shared high identity with group A MAPK in plants. A time course(30-240 min)experiment using a variety of abiotic stresses revealed that the transcripts level of ZmMPK7 accumulated markedly and rapidly when maize seedlings were subjected to exogenous signaling molecules:ABA, H2O2, various abiotic stimuli such as drought, salinity, cold, heavy metal. Its transcription has no tissue-specificity. Finally, we have shown that the group A ZmMPK7 protein was localized in the cytoplasm. These results suggest that the ZmMPK7 may play an important role in response to environmental stresses and transcription of certain genes.

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备注/Memo

备注/Memo:
国家自然科学基金项目(30671247)
更新日期/Last Update: 2011-01-30