[1]路娟,吴俊,张绍铃,等.不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011,34(2):38-46.[doi:10.7685/j.issn.1000-2030.2011.02.008]
 LU Juan,WU Jun,ZHANG Shao-ling,et al.Genetic diversity and polygentic relationship among pears reveled by SSR analysis[J].Journal of Nanjing Agricultural University,2011,34(2):38-46.[doi:10.7685/j.issn.1000-2030.2011.02.008]
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不同系统梨种质遗传多样性与分类关系的SSR分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年2期
页码:
38-46
栏目:
出版日期:
2011-03-31

文章信息/Info

Title:
Genetic diversity and polygentic relationship among pears reveled by SSR analysis
作者:
路娟吴俊张绍铃吴华清张好艳
南京农业大学园艺学院,江苏南京210095;江苏省农业科学院园艺研究所,江苏南京210014
Author(s):
LU Juan WU Jun ZHANG Shao-ling WU Hua-qing ZHANG Yu-yan
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
遗传多样性 亲缘关系 SSR
Keywords:
Pyrus genetic diversity polygenetic relationship SSR
分类号:
S661.2
DOI:
10.7685/j.issn.1000-2030.2011.02.008
文献标志码:
A
摘要:
利用SSR分子标记技术深入探讨了梨主要栽培种白梨(Pyrus bretschneideri Rehd.)、砂梨(Pyrus pyrifolia(Burm.f.)Na-kai)、秋子梨(Pyrus ussuriensis Maxim.)、西洋梨(Pyrus communis L.)、新疆梨(Pyrus sinkiangensis Yü)和野生种以及种间杂交新选育品种共150份资源的遗传多样性、亲缘关系以及系统分类地位。通过筛选出的25对SSR引物共检测到407个位点,不同引物检测位点数5~25个,其中多态性位点数390个,占检测位点的95.82%,检测位点杂合度为0.4~0.7,不同品种间的遗传多样性指数为0.67~1.00。应用NTSYS-pc2.01软件,以非加权数据分析法(UPGMA)对获得的多态性位点进行聚类分析,系统聚类结果将150份梨种质分为2大类群,即西洋梨和东方梨类群。西洋梨类群包括了西洋梨品种及具西洋梨亲缘的种间杂交品种。东方梨类群包括了中国白梨、砂梨、秋子梨、新疆梨、野生资源以及日韩梨品种,在相似系数0.708处又分为2大组,并可细分为7个亚组。其中,中国的白梨、砂梨和秋子梨相互交错在一起,没有独自成组。中国砂梨表现出最丰富的遗传多样性;其次是中国白梨。日本梨与中国砂梨表现了高度的亲缘关系,并且没有独立成组。多数新选育品种的遗传关系表现出趋母本聚类型或趋父本聚类型。
Abstract:
Genetic diversity and polygentic relationship of 150 cultivars of pear, which belong to Pyrus bretschneideri Rehd. , Pyrus pyrifolia(Burm. f. )Nakai, Pyrus ussuriensis Maxim. , Pyrus communis L. , Pyrus sinkiangensis Yü. , wild species and inter-specific hybridization cultivars, were analyzed by SSR markers in this paper. 25 primer-pairs produced a total of 407 alleles, of which 390 were polymorphic with a polymorphism percentage of 95.82%. Prime conbination were 5-25, Heterozygosity ratio was 0.4-0.7, and the similarity coefficient between cultivars ranged from 0.67 to 1.00.The dendrogram generated from all the 150 accessions by unweighted pair-group method of arithmetic analysis(UPGMA)cluster analysis clearly distinguished Occidental pears from accessions of East Asia. Cultivars of P. communis and inter-specific hybridization with P. communis are clustered into the Occidental clade. The clade of oriental pear formed two main groups, seven subgroups, at the coefficient of 0.708.P. bretschneideri, P. ussuriensis and P. pyrifolia originating in China interveined each other, and did not group independently. Chinese P. pyrifolia showed the most abundant genetic diversity, followed by P. bretschneideri. The cultivars from Japan and the Chinese P. pyrifolia demonstrated a high degree of genetic relationship, and there is no separate group. The genetic relationship of most new cultivar selections show increasingly female parent type or increasingly male parent poly-poly-type.

参考文献/References:

[1]Oliveira,C,M,Monte-Corvo,L,Monte-Corvo,L. Molecular typing of Pyrus based on RAPD markers[J]. Scientia Horticulturae, 1999
[2]Monte-Corvo,L,Oliveira,C,Oliveira,C. Assessment of genetic relationships among Pyrus species and cuhivars using AFLP and RAPD markers[J]. Genetic Resources and Crop Evolution, 2000
[3]龚桂芝,彭祝春. 枳属种质遗传多样性及其与近缘属植物亲缘关系的SSR和cpSSR分析[J]. 园艺学报, 2008
[4]Kimura,T,Shoda,M,Shoda,M. Identification of Asian pear varieties by SSR analysis[J]. Breeding Science, 2002
[5]Lin,Sheng-hua,Song,Wen-qin,Song,Wen-qin. AFLP molecular markers of 10 species of Pyrus in China[J]. Acta Horticulturae, 2002
[6]G(o)kirmak,T,Bassil,N,V,Bassil,N,V. Characterization of European hazelnut(Corylus avellana)cultivars using SSR markers[J]. Mehienbacher Genet Resour Crop Evol, 2009
[7]Bassil,N,Postman,J,D,Postman,J,D. Nomenclature and genetic relationships of apples and pears from Terceira Island[J]. Genetic Resources and Crop Evolution, 2009
[8]沈志军,俞明亮. 无锡水蜜桃品种群遗传多样性及与其他群体亲缘关系的SSR分析[J]. 植物遗传资源学报, 2009
[9]吴子龙,沈育杰. 山葡萄种内遗传多样性的SSR分析[J]. 果树学报, 2008
[10]Yamamoto,T,Sawamura,Y,Sawamura,Y. SSRs isolated from apple can identify polymorphism and genetic diversity in pear[J]. Theoretical and Applied Genetics, 2001
[11]易芍文,周小罡. 云南省梨属植物资源的微卫星分析研究[J]. 西南农业学报, 2004
[12]曹玉芬,高源. 梨栽培品种SSR鉴定及遗传多样性[J]. 园艺学报, 2007
[13]范太伟,李红旭. 甘肃中部梨资源遗传变异和亲缘关系的SSR分析[J]. 果树学报, 2007
[14]张东. 中国红皮砂梨遗传多样性及亲缘关系的SSR和AFLP分析[D]. 杭州:浙江大学, 2006
[15]张妤艳,张绍铃. 基于SSR标记的梨资源遗传多样性分析[J]. 农业生物技术学报, 2008
[16]程丽莉,秦岭. 板栗叶片DNA的提取及AFLP反应体系的建立[J]. 北京农学院学报, 2005
[17]Yamamoto,T,Sawamura,Y,Sawamura,Y. Simple sequence repeats for genetic analysis in pear[J]. Euphytica, 2002
[18]Nei,M. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Sciences of the United States of America, 1979
[19]Roll,F,J. Numerical taxonomy and multivariate analysis system. Version 2. 0. Exeter Software,Setauket[M]. New York, 1998
[20]Teng,Y,W,Tamum,F,Tamum,F. Genetic relationships of Pyrus species and euhivars native to East Asia revearled by randomly amplified polymorphie DNA markers[J]. Journal of the American Society for Horticultural Science, 2002
[21]Rubtsov,G,A. Geographical distribution of the genus Pyrus and trends and factors in its evolution[J]. American Naturalist, 1944
[22]胡昌炽. Classification study on east Asian Pyrus[J]. 中华农学会报, 1933
[23]梶浦一郎. ニホソナシの育種及びその基礎研究と栽培品種の来屋及び特性[R]果樹試驗場報告:特別報告第1号, 1990
[24]蒲富慎,孙秉均. 梨品种[M]. 北京:中国农业出版社, 1989
[25]杨槐俊. 孢粉学在部分梨属植物分类研究中的应用[J]. 果树科学, 1985
[26]邹乐敏,张志德. 根据花粉形态探讨梨属植物的亲缘关系[J]. 园艺学报, 1986
[27]Teng,Y,Tamura,F,Tamura,F. Genetic relationships ofpear eultivars in Xinjiang,China asmeasured by RAPD markers[J]. Journal of Hortieuhural Science and Biotechnology, 2001
[28]王斐,方成泉. 梨新品种及其亲本的AFLP分析[J]. 园艺学报, 2007

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备注/Memo

备注/Memo:
国家公益性行业(农业)科研专项经费(200903044);国家自然科学基金项目(30900974);国家梨产业技术体系建设专项经费(CARS-29)
更新日期/Last Update: 2011-03-25