[1]丁举静,何成华,刘俐君,等.脱氧雪腐镰刀菌烯醇对BHK-21细胞的线粒体膜电位及Bax和Bcl-2蛋白表达的影响[J].南京农业大学学报,2011,34(2):101-106.[doi:10.7685/j.issn.1000-2030.2011.02.018]
 DING Ju-jing,HE Cheng-hua,LIU Li-jun,et al.Effect of deoxynivalenol on the mitochondrial membrane potentials and Bax,Bcl-2 expression of BHK-21 cells[J].Journal of Nanjing Agricultural University,2011,34(2):101-106.[doi:10.7685/j.issn.1000-2030.2011.02.018]
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脱氧雪腐镰刀菌烯醇对BHK-21细胞的线粒体膜电位及Bax和Bcl-2蛋白表达的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年2期
页码:
101-106
栏目:
出版日期:
2011-03-31

文章信息/Info

Title:
Effect of deoxynivalenol on the mitochondrial membrane potentials and Bax,Bcl-2 expression of BHK-21 cells
作者:
丁举静何成华刘俐君马彦娜张海彬
南京农业大学动物医学院,江苏南京210095;南京日升昌康华药业有限公司,江苏南京210007
Author(s):
DING Ju-jing HE Cheng-hua LIU Li-jun MA Yan-na ZHANG Hai-bin
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Nanjing Risingsun Biotechnology Co., Ltd., Nanjing 210007, China
关键词:
脱氧雪腐镰刀菌烯醇 仓鼠肾细胞(BHK-21) 线粒体膜电位 细胞凋亡 Bax Bcl-2
Keywords:
deoxynivalenol(DON) hamster kidney cells(BHK-21) mitochondrial membrane potentials(MMP) apoptosis Bax Bcl-2
分类号:
S432.4
DOI:
10.7685/j.issn.1000-2030.2011.02.018
文献标志码:
A
摘要:
为了探讨脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)对体外培养仓鼠肾细胞(BHK-21)的线粒体膜电位及Bax和Bcl-2蛋白表达的影响,采用细胞培养、流式细胞术、免疫细胞化学染色等方法,研究DON对细胞早期凋亡率、线粒体膜电位及Bax和Bcl-2蛋白表达的变化。结果显示:不同浓度的DON均可诱导BHK-21细胞凋亡,各浓度组的凋亡率都显著高于对照组(P<0.05);DON可使BHK-21细胞线粒体膜电位下降,且表现出剂量和时间效应关系。免疫组织化学染色显示,DON处理细胞24 h,Bax蛋白表达显著高于对照组,而Bcl-2蛋白表达均低于对照组。上述结果表明:DON诱导了BHK-21细胞凋亡和线粒体膜电位下降,Bax表达增强而Bcl-2表达下降是DON诱导BHK-21细胞凋亡的分子机制之一。
Abstract:
To investigate the effect of deoxynivalenol(DON)on the mitochondrial membrane potentials(MMP)and Bax, Bcl-2 expression of hamster kidney cells(BHK-21 cell)in vitro, the change of early apoptosis, mitochondrial membrane potential and apoptosis-related proteins with Bax and Bcl-2 expression were measured by flow cytometry and immunocytochemical staining assay. The results showed that different concentration of DON could induce apoptosis for BHK-21 cells within the experimental concentration and cause apoptosis significantly as compared with control group(P<0.05). A marked decrease of mitochondrial membrane potential in a dose and time dependent manner were observed clearly by mitochondrial membrane potentials assay. Immunohistochemical staining analysis showed that, after 24 h treatment with DON, the expression of Bax was significantly higher, while Bcl-2 decreased. These result suggested that DON could induce apoptosis and the MMP disruptionin of BHK-21 cells in vitro. The essential mechanisms of apoptosis might be related to inducing the change of hepatocarcinoma cell cycle, the expression of the Bax increase and Bcl-2 decrease in BHK-21 cells.

参考文献/References:

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备注/Memo

备注/Memo:
国家863计划项目(2007AA10Z429);国家自然科学基金项目(30771595,30901091);江苏省农业科技自主创新基金项目(CX(08)135)
更新日期/Last Update: 2011-03-25