[1]张爱芬,王立,侯喜林,等.不结球白菜S 位点受体激酶基因片段的克隆与表达分析[J].南京农业大学学报,2011,34(3):25-30.[doi:10.7685/j.issn.1000-2030.2011.03.005]
 ZHANG Ai-fen,WANG Li,HOU Xi-lin,et al.Cloning and expression analysis of SRK gene fragment in non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2011,34(3):25-30.[doi:10.7685/j.issn.1000-2030.2011.03.005]
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不结球白菜S 位点受体激酶基因片段的克隆与表达分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年3期
页码:
25-30
栏目:
出版日期:
2011-05-31

文章信息/Info

Title:
Cloning and expression analysis of SRK gene fragment in non-heading Chinese cabbage
作者:
张爱芬王立侯喜林刘同坤李英*
南京农业大学作物遗传与种质创新国家重点实验室/ 农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095
Author(s):
ZHANG Ai-fen WANG Li HOU Xi-lin LIU Tong-kun LI Ying
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
不结球白菜S 位点受体激酶基因荧光定量PCR基因表达分析
Keywords:
Brassica campestris ssp.chinensis Makino S locus receptor kinase gene real-time PCR quantitative analysis of gene expression
分类号:
S634. 3
DOI:
10.7685/j.issn.1000-2030.2011.03.005
文献标志码:
A
摘要:
以不结球白菜自交不亲和系03 的基因组DNA 和柱头cDNA 为模板,利用引物SRKF/ SRKR 扩增获得长度为964 bp 和646 bp 的SRK 基因片段。序列比较分析表明,克隆的基因片段属于SRK 基因的激酶域,该序列包含4 个外显子和3 个内含子,编码215 个氨基酸,与芜菁、甘蓝、芥蓝等SRK 基因有90%以上的相似性。荧光定量PCR 分析结果表明:自交不亲和系03 和自交亲和系105 不同组织中SRK 基因的表达水平存在显著的差异,SRK 基因主要在自交不亲和系的柱头中高度表达,自交亲和系中该基因的表达主要分布于叶片、花蕾和柱头组织中。
Abstract:
Using primers SRKF/SRKR, 964 bp gDNA(BcSRK-1)and 646 bp cDNA sequences of S locus receptor kinase gene were obtained from self-incompatible line 03 of Brassica campestris ssp. chinensis Makino. Sequence alignment and phylogenetic analysis revealed that DNA and cDNA of BcSRK-1 belonged to kinase domain, containing 4 exons and 3 introns, and coded 215 amino acids. Furthermore, its similarity was over 90% with the SRK gene of Brassica rapa, Brassica oleracea and Brassica oleracea var. alboglabra. Real-time PCR analysis showed there was a significant difference between self-incompatible and self-compatible lines. BcSRK-1 was highly expressed in stigmas of self-incompatible line. Howerer, in self-compatible line, BcSRK-1 was expressed in an even lower level whether in stigmas, buds or leaves.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-10-09 基金项目:国家973 计划项目子课题(2009CB119001-04);教育部新世纪优秀人才支持计划项目(NCET-07-0439) 作者简介:张爱芬,硕士研究生。*通讯作者: 李英,教授,从事蔬菜遗传育种及分子生物学研究,E-mail:yingli@ njau. edu. cn。
更新日期/Last Update: 2011-05-25