[1]张振兴,孙锦,郭世荣*,等.增施钙素对盐胁迫下西瓜幼苗生长和可溶性蛋白含量及组分的影响[J].南京农业大学学报,2011,34(5):20-24.[doi:10.7685/j.issn.1000-2030.2011.05.004]
 ZHANG Zhen-xing,SUN Jin,GUO Shi-rong*,et al.Effect of supplementary calcium on the growth and the content and components of soluble protein of watermelon seedlings under salt-stress[J].Journal of Nanjing Agricultural University,2011,34(5):20-24.[doi:10.7685/j.issn.1000-2030.2011.05.004]
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增施钙素对盐胁迫下西瓜幼苗生长和可溶性蛋白含量及组分的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年5期
页码:
20-24
栏目:
出版日期:
2011-10-18

文章信息/Info

Title:
Effect of supplementary calcium on the growth and the content and components of soluble protein of watermelon seedlings under salt-stress
作者:
张振兴孙锦郭世荣*王丽萍童辉
南京农业大学园艺学院/农业部南方蔬菜遗传改良重点开放实验室,江苏 南京 210095
Author(s):
ZHANG Zhen-xingSUN JinGUO Shi-rong*WANG Li-pingTONG Hui
College of Horticulture/Key Laboratory of Southern Vegetable Crop Genetics Improvement, Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China
关键词:
西瓜盐胁迫可溶性蛋白生物量
Keywords:
Citrullus lanatus Mansfeldsalt-stresscalciumsoluble proteinbiomass
分类号:
S651
DOI:
10.7685/j.issn.1000-2030.2011.05.004
文献标志码:
A
摘要:
采用水培方式,以盐敏感型的西瓜(Citrullus lanatus Mansfeld)品种‘秀丽’为试材,研究了营养液增补Ca2+对盐胁迫下西瓜幼苗生物量、可溶性蛋白含量和组分的影响。结果表明:50 mmol?L-1 NaCl胁迫下,营养液中Ca2+浓度由4 mmol?L-1增补到6和8 mmol?L-1时可明显提高盐胁迫幼苗植株的生物量,其分别达到对照水平的78.17%和89.50%,根系可溶性蛋白含量分别提高到对照水平的89.1%和104.3%,叶片可溶性蛋白含量分别提高到对照水平的92.4%和103.7%。同时,叶片28.5×103蛋白组分升高,107.4×103蛋白组分降低,根系的多种蛋白组分升高接近对照水平。因此,Ca2+对这些蛋白组分的表达影响作用可能是其缓解西瓜植株盐胁迫伤害的原因之一。
Abstract:
Salinity-sensitive mini-watermelon(Citrullus lanatus Mansfeld)cultivar‘Xiuli’was used to study the effect of supplementary calcium on the growth and the content and components of soluble protein of watermelon seedlings grown under salt-stress by applying additional calcium to hydroponic nutrient solution.The results showed that the increasing Ca2+ concentration from 4 mmol?L-1 to 6 and 8 mmol?L-1 could significantly increase biomass and soluble protein content of seedlings grown under 50 mmol?L-1 NaCl stress.Increasing Ca2+ concentration increased the salt-stressed plant biomass to 78.2%,89.5% of control respectively,and soluble leaf protein content to 89.1%,104.3% of control,and soluble root protein content to 92.4%,103.7% of control.Moreover,increasing Ca2+ concentration up-regulated 28.5×103 protein component and down-regulated 107.4×103 protein component in leaf,and up-regulated several root proteins components,nearly the same as the control.Therefore,the effects of supplementary calcium on these proteins components expression are possibly one of important reasons why calcium can alleviate salt-stress damage in watermelon seedlings.

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备注/Memo

备注/Memo:
收稿日期:2010-11-09 基金项目:国家重点基础研究发展计划项目(2009CB119000);国家自然科学基金项目(30900995,31071831);江苏省农业三项工程项目(SX(2008)026);现代农业产业技术体系建设专项资金资助项目(Nycytx-35-gw18) 作者简介:张振兴,硕士研究生。*通讯作者:郭世荣,教授,主要从事作物逆境生理和设施园艺与无土栽培方面的研究, E-mail:srguo@njau.edu.cn。
更新日期/Last Update: 2011-10-31