[1]曾后清,刘赣,朱毅勇*,等.拟南芥体内硝酸盐积累差异与细胞膜H+-ATPase的关系[J].南京农业大学学报,2011,34(6):89.[doi:10.7685/j.issn.1000-2030.2011.06.016]
 ZENG Hou-qing,LIU Gan,ZHU Yi-yong*,et al.The relationship between the accumulation of nitrate and plasma membrane H+-ATPase in Arabidopsis[J].Journal of Nanjing Agricultural University,2011,34(6):89.[doi:10.7685/j.issn.1000-2030.2011.06.016]
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拟南芥体内硝酸盐积累差异与细胞膜H+-ATPase的关系()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年6期
页码:
89
栏目:
出版日期:
2011-11-30

文章信息/Info

Title:
The relationship between the accumulation of nitrate and plasma membrane H+-ATPase in Arabidopsis
作者:
曾后清刘赣朱毅勇*徐国华沈其荣
南京农业大学资源与环境科学学院,江苏 南京 210095
Author(s):
ZENG Hou-qingLIU GanZHU Yi-yong*XU Guo-huaSHEN Qi-rong
College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China
关键词:
拟南芥硝酸盐细胞膜H+-ATPase
Keywords:
Arabidopsisnitrateplasma membrane H+-ATPase
分类号:
Q556+.9
DOI:
10.7685/j.issn.1000-2030.2011.06.016
摘要:
以野生型拟南芥(WT)和叶柄硝酸盐积累减少的拟南芥突变体atnrt1.4为材料,研究了叶柄与叶片硝酸盐的积累及细胞膜H+-ATPase。采用两相法分离了2种材料细胞膜并测定了细胞膜H+-ATPase活性,通过RT-PCR分析了WT与atnrt1.4叶柄及叶片中各个细胞膜H+-ATPase基因的表达,并采用Western blot分析了WT与atnrt1.4叶柄及叶片细胞膜上H+-ATPase的蛋白浓度。结果显示:atnrt1.4叶柄硝酸盐积累显著低于WT叶柄,仅为WT的58.6%;atnrt1.4叶柄中细胞膜H+-ATPase基因AHA3、AHA5、AHA10和AHA11的表达比WT显著降低;atnrt1.4叶柄细胞膜H+-ATPase蛋白浓度也显著低于WT叶柄。WT叶柄硝酸盐积累显著高于叶片,相应的细胞膜H+-ATPase的水解活性也显著高于叶片。上述研究表明:atnrt1.4叶柄硝酸盐积累减少导致其细胞膜H+-ATPase活性降低;拟南芥体内硝酸盐积累差异与细胞膜H+-ATPase活性有关。
Abstract:
Arabidopsis nitrate transporter mutant atnrt1.4,which has a very low nitrate concentration in petiole,and wild type Arabidopsis(WT)were used as materials to investigate the relationship between the distribution of nitrate in plant tissues and plasma membrane H+-ATPase.The plasma membrane vesicles of petiole and lamina of atnrt1.4 and WT were isolated,respectively,by two-phase system for the determination of H+-ATPase activity.The expression of plasma membrane H+-ATPase genes was analyzed by RT-PCR and the enzyme concentration of plasma membrane H+-ATPase was detected by Western blot.Results showed:the nitrate concentration of atnrt1.4 petiole was only 58.6% of WT petiole,and H+-ATPase activity of atnrt1.4 petiole was much lower than WT petiole;the decreased expression of plasma membrane H+-ATPase genes,AHA3,AHA5,AHA10 and AHA11,were responsible for the lower plasma membrane H+-ATPase activity in atnrt1.4 petiole;in addition,enzyme concentration of plasma membrane H+-ATPase of atnrt1.4 petiole was evidently lower than WT petiole;the nitrate concentration of WT petiole was higher than WT lamina,and the corresponding plasma membrane H+-ATPase activity of WT petiole was also higher than WT lamina.The results indicated that the lower activity of plasma membrane H+-ATPase in atnrt1.4 petiole was attributed to the less accumulation of nitrate,and the accumulation of nitrate was related to plasma membrane H+-ATPase in Arabidopsis.

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备注/Memo

备注/Memo:
收稿日期:2011-05-31基金项目:国家自然科学基金资助项目(30971864)作者简介:曾后清,博士研究生。*通讯作者:朱毅勇,副教授,主要从事植物营养学研究,E-mail:yiyong1973@njau.edu.cn。
更新日期/Last Update: 2011-12-06