[1]宋晓宏,沙伟*,金忠民,等.毛尖紫萼藓GpAPX的克隆和信息学分析及其在植株干旱与复水下的表达分析[J].南京农业大学学报,2012,35(2):51-58.[doi:10.7685/j.issn.1000-2030.2012.02.009]
 SONG Xiao-hong,SHA Wei*,JIN Zhong-min,et al.Cloning,bioinformatics analysis and expression analysis of GpAPX during dehydration and rehydration in Grimmia pilifera[J].Journal of Nanjing Agricultural University,2012,35(2):51-58.[doi:10.7685/j.issn.1000-2030.2012.02.009]
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毛尖紫萼藓GpAPX的克隆和信息学分析及其在植株干旱与复水下的表达分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
35卷
期数:
2012年2期
页码:
51-58
栏目:
OA栏目
出版日期:
2012-03-25

文章信息/Info

Title:
Cloning,bioinformatics analysis and expression analysis of GpAPX during dehydration and rehydration in Grimmia pilifera
作者:
宋晓宏1 沙伟2* 金忠民2 张艳馥2
1. 东北林业大学生命科学学院, 黑龙江 哈尔滨 150040;
2. 齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006
Author(s):
SONG Xiao-hong1 SHA Wei2* JIN Zhong-min2 ZHANG Yan-fu2
1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China;
2. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
关键词:
毛尖紫萼藓干旱胁迫GpAPX序列分析基因表达
Keywords:
Grimmia piliferadrought stressGpAPXsequence analysisgene expression
分类号:
Q949.35+2
DOI:
10.7685/j.issn.1000-2030.2012.02.009
文献标志码:
A
摘要:
以毛尖紫萼藓(Grimmia pilifera)为试材,采用RACE技术克隆获得1个抗坏血酸过氧化物酶基因(APX)的cDNA序列,命名为GpAPX(GenBank登录号:GU989311)。该基因全长1 115 bp,编码区为771 bp,编码256个氨基酸,5’非翻译区为116 bp,3’非翻译区为228 bp。序列分析表明:该基因属于过氧化物酶基因家族,与其他植物抗坏血酸过氧化物酶的同源性为70.2%~89.7%。实时定量PCR检测结果显示,GpAPX在干旱及复水条件下均能表达,表明GpAPX蛋白可能与抗旱性相关,在毛尖紫萼藓响应干旱胁迫过程中发挥作用。
Abstract:
In this study,a full-length cDNA sequence of a ascorbate peroxidase(APX)gene named GpAPX was cloned from Grimmia pilifera(GenBank accession No.GU989311)using rapid amplification of cDNA ends(RACE).The full-length cDNA of GpAPX was 1 115 bp with a 771 bp open reading frame(ORF)which encoded 256 amino acids and containing a 116 bp 5’UTR and a 228 bp 3’UTR.Sequence analysis showed that GpAPX belonged to the peroxidase protein families.GpAPX protein sharing 70.2%-89.7% homology with APX proteins from other plants.QRT-PCR analysis suggested that the expression of GpAPX gene was induced in both dehydration and rehydration.The results demonstrated that the GpAPX protein may be involved in drought resisitance,and play an important role in Grimmia pilifera through responses to drought stress.

参考文献/References:

[1] Badawi G H,Yamauchi Y,Shimada E,et al.Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco(Nicotiana tabacum)chloroplasts[J].Plant Science,2004,166(4):919-928
[2] Guo Z,Ou W,Lu S,et al.Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity[J].Plant Physiology and Biochemistry,2006,44(11/12):828-836
[3] Foyer C H,Halliwell B.Purification and properties of dehydroascorbate reductase from spinach leaves[J].Phytochemistry,1977,16(9):1347-1350
[4] Nakano Y,Asada K.Hydrogen peroxide is scavenged byascorbate specific peroxidase in spinach chloroplasts[J].Plant Cell Physio1,1981,22(5):867-880
[5] Teixeira F K,Menezes-Benavente L,Margis R,et al.Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family:inferences from the rice genome[J].Journal of Molecular Evolution,2004,59(6):761-770
[6] Shigeoka S,Ishikawa T,Tamoi M,et al.Regulation and function of ascorbate peroxidase isoenzymes[J].Journal of Experimental Botany,2002,53(372):1305-1319
[7] Shi W M,Muramoto Y,Ueda A,et al.Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana[J].Gene,2001,273(1):23-27
[8] Ma L B,Zhang F Y,Ma C Y,et al.cDNA cloning and sequence characterization of an ascorbate eroxidase from marine red alga Porphyra yezoensis[J].Journal of Fishery Sciences of China,2005,12(3):281-287
[9] Mittler R,Zilinskas B A.Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase[J].The Journal of Biological Chemistry,1992,267(30):21802-21807
[10] Orvar B L,Ellis B E.Isolation of a cDNA encoding cytosolic ascorbate peroxidase in tobacco[J].Plant Physiol,1995,108(2):839-840
[11] Webb R P,Allen R D.Isolation and characterization of a cDNA for spinach cytosolic ascorbate peroxidase[J].Plant Physiology,1995,108(3):1325
[12] Jespersen H M,Kjaersgåird I V H,stergaard L,et al.From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure,function and evolution of seven types of ascorbate peroxidase[J].Biochemical Engineering Journal,1997,326:305-310
[13] Soares da Costa D,Pereira C S,Teixeira J,et al.Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants(Solanum tuberosum L.)[J].Acta Physiologiae Plantarum,2006,28(1):41-47
[14] Lu Z Q,Takano T,Liu S K.Purification and characterization of two ascorbate peroxidases of rice(Oryza sativa L.)expressed in Escherichia coli[J].Biotechnology Letters,2005,27(1):63-67
[15] 李筠,邓西平,郭尚洙,等.转铜/锌超氧化物歧化酶和抗坏血酸过氧化物酶基因甘薯的耐旱性[J].植物生理与分子生物学学报,2006,32(4):451-457
[16] Kornyeyev D,Logan B A,Payton P,et al.Enhanced photochemical light utilization and decreased chilling-induced photoinhibition of photosystemⅡin cotton overexpression genes encoding chloroplast-targeted antioxidant enzymes[J].Physiologia Plantarum,2001,113(3):323-331
[17] Tang L,Tang H,Sang-Soo K,et al.Improving potato plants oxidative stress and salt tolerance by gene transfer both of Cu/Zn superoxide dismutase and ascorbate peroxidase[J].China Biotechnology,2008,28(3):25-31
[18] Lu Z Q,Liu D L,Liu S K.Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis[J].Plant Cell Reports,2007,26(10):1909-1917
[19] Yan J Q,Wang J,Tissue D,et al.Photosynthesis and seed production under water-deficit conditions in transgenic tobacco plants that overexpression an Arabidopsis ascorbate peroxidase gene[J].Crop Science,2003,43(4):1477-1483
[20] Lee Y P,Kim S H,Bang J W,et al.Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts[J].Plant Cell Reports,2007,26(5):591-598
[21] 孙卫红,李风,束德峰,等.转番茄正义抗坏血酸过氧化物酶基因提高烟草耐盐能力[J].中国农业科学,2009,42(4):1165-1171
[22] 韩瑞丽,陆海.转APX基因烟草抗旱能力研究[J].成都大学学报:自然科学版,2007,26(2):93-96
[23] Bewley J D.Physiological aspects of desiccation tolerance[J].Annual Review of Plant Physiology,1979,30:195-238
[24] 吴鹏程.苔藓植物生物学[M].北京:科学出版社,1998:131-147
[25] Oliver M J,Bewley J D.Plant desiccation and protein synthesis Ⅳ:RNA synthesis,stability,and recruitment of RNA into protein synthesis during desiccation and rehydration of the desiccation-tolerant moss,Tortula ruralis[J].Plant Physiol,1984,74(1):21-25
[26] Bewley J D.Polyribosomes conserved during desiccation of the moss Tortula ruralis are active[J].Plant Physiol,1973,51(2):285-288
[27] Hinshiri H M,Proctor M C F.The effect of desiccation on subsequent assimilation and respiration of the bryophytes Anomodon viticulosus and Porella platyphylla[J].New Phytol,1971,70(3):527-538
[28] Oliver M J.Influence of protoplasmic water loss on the control of protein synthesis in the desiccation-tolerant moss Tortula ruralis:ramifications for a repair-based mechanism of desiccation-tolerance[J].Plant Physiol,1991,97(4):1501-1511
[29] Oliver M J,Velten J,Wood A J.Bryophytes as experimental models for the study of environmental stress tolerance:Tortula ruralis and desiccation-tolerance in mosses[J].Plant Ecology,2000,151(1):73-84
[30] 张萍,白学良.耐旱苔藓植物生理学及分子生物学研究进展[J].内蒙古大学学报:自然科学版,2004,35(2):227-233
[31] Scott H B Ⅱ,Oliver M J.Accumulation and polysomal recruitment of transcripts in response to desiccation and rehydration of the moss Tortula ruralis[J].Journal of Experimental Botany,1994,45(5):577-583
[32] Oliver M J,Bewley J D.Plant desiccation and protein synthesis Ⅳ:Changes in protein synthesis elicited by desiccation of the moss Tortula ruralis are effected at the translational level[J].Plant Physiol,1984,74(4):923-927
[33] Wood A J,Oliver M J.Translational control in plant setess:the formation of messenger ribonucleoprotein particles(mRNPs)in response to desiccation of Tortula ruralis gametophytes[J].The Plant Journal,1999,18(4):359-370
[34] Skiba B,Ford R,Pang E C K.Construction of a cDNA library of Lathyrus sativus inoculated with Mycosphaerella pinodes and the expression of potential defence-related expressed sequence tags(ESTs)[J].Physiological and Molecular Plant Pathology,2005,66(1/2):55-67
[35] 马金彪,王晓杰,于秀梅,等.条锈菌诱导的小麦叶片cDNA文库构建及表达序列标签分析[J].植物病理学报,2007,37(3):265-270

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备注/Memo

备注/Memo:
收稿日期:2011-08-25
基金项目:黑龙江省科技厅农业攻关项目(GC06J101);黑龙江省教育厅海外学人合作项目(1151hz022)
作者简介:宋晓宏,博士研究生。*通讯作者:沙伟,教授,博导,从事苔藓植物遗传学和分子生物学研究,E-mail:shw1129@263.net。
更新日期/Last Update: 1900-01-01