[1]刘国红,梁明祥,周诗雨,等.甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究[J].南京农业大学学报,2012,35(3):42-46.[doi:10.7685/j.issn.1000-2030.2012.03.008]
 LIU Guo-hong,LIANG Ming-xiang,ZHOU Shi-yu,et al.The comparative study on different explant regeneration systems in canola‘Nanyanyou 1’(Brassica napus L.)[J].Journal of Nanjing Agricultural University,2012,35(3):42-46.[doi:10.7685/j.issn.1000-2030.2012.03.008]
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甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
35卷
期数:
2012年3期
页码:
42-46
栏目:
出版日期:
2012-05-18

文章信息/Info

Title:
The comparative study on different explant regeneration systems in canola‘Nanyanyou 1’(Brassica napus L.)
作者:
刘国红 梁明祥 周诗雨 郑青松 刘兆普
南京农业大学资源与环境科学学院/江苏省海洋生物学重点实验室, 江苏 南京 210095
Author(s):
LIU Guo-hong LIANG Ming-xiang ZHOU Shi-yu ZHENG Qing-song LIU Zhao-pu
College of Resources and Environmental Sciences/Key Laboratory of Marine Biology, Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China
关键词:
甘蓝型油菜下胚轴具柄子叶再生体系
Keywords:
Brassica napus L.hypocotylcotyledon with petioleregeneration system
分类号:
S565.4
DOI:
10.7685/j.issn.1000-2030.2012.03.008
文献标志码:
A
摘要:
以甘蓝型油菜‘南盐油1号’下胚轴和具柄子叶为材料,通过植物组织培养方法,探讨不同预培养和分化培养的交叉组合对油菜植株再生的影响。结果表明:不同浓度的2,4-D、6-BA和AgNO3组合对外植体的分化有不同程度的影响,但分化出的芽苗均能生根,并生长成为植株。下胚轴在预培养(MS+0.5 mg·L-1 2,4-D+1.0 mg·L-1 6-BA)和分化培养(MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+5.0 mg·L-1 AgNO3)条件下的分化率最高,达到95.23%;具柄子叶在预培养(MS+0.5 mg·L-1 2,4-D+1.0 mg·L-1 6-BA)和分化培养(MS+4.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5.0 mg·L-1 AgNO3)下的分化率最高,达到89.52%。上述结果表明,下胚轴很可能更适合作为‘南盐油1号’油菜在组织培养研究中的外植体。
Abstract:
The plant tissue culture was tried in the experiment,in which hypocotyl and the cotyledon with petiole of‘Nanyanyou 1’(Brassica napus L.)were used as materials.The effects of cross combination of the different pre-culture and differentiation culture on the canola plant regeneration were studied.The results showed that different concentrations of 2,4-D,6-BA and AgNO3 had varing degree of influences on explant differentiation,however,differentiated buds were all able to take root and grow into plant.The differentiation rate of hypocotyl achieved the peak value(95.23%)in the pre-culture(MS+0.5 mg·L-12,4-D+1.0 mg·L-1 6-BA)and differentiation(MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+5.0 mg·L-1 AgNO3);the cotyledon with petiole achieved the peak value(89.52%)in pre-culture(MS+0.5 mg·L-1 2,4-D+1.0 mg·L-1 6-BA)and differentiation(MS+4.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5.0 mg·L-1 AgNO3).To sum up,compared with the cotyledon with petiole,hypocotyl might be more suitable as the explant in tissue culture research of‘Nanyanyou 1’.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-12-11
基金项目:国家科技支撑计划项目(2011BAD13B09);作物遗传与种质创新国家重点实验室开放课题(ZW2010004);校青年科技创新基金项目(Y201058)
作者简介:刘国红,硕士研究生。
通讯作者:刘兆普,教授,主要从事滩涂农业资源研究,E-mail:sea@njau.edu.cn。
更新日期/Last Update: 1900-01-01