[1]孙江慧,张楠,沈其荣,等.几种食用真菌降解稻草的潜力研究[J].南京农业大学学报,2012,35(6):49-54.[doi:10.7685/j.issn.1000-2030.2012.06.009]
 SUN Jiang-hui,ZHANG Nan,SHEN Qi-rong,et al.Study on biodegrated ability of edible fungi to rice straw[J].Journal of Nanjing Agricultural University,2012,35(6):49-54.[doi:10.7685/j.issn.1000-2030.2012.06.009]
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几种食用真菌降解稻草的潜力研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
35卷
期数:
2012年6期
页码:
49-54
栏目:
出版日期:
2012-11-15

文章信息/Info

Title:
Study on biodegrated ability of edible fungi to rice straw
作者:
孙江慧 张楠 沈其荣 沈标
南京农业大学江苏省固体有机废弃物资源化利用高技术研究重点实验室, 江苏 南京 210095
Author(s):
SUN Jiang-hui ZHANG Nan SHEN Qi-rong SHEN Biao
Jiangsu Key Laboratory for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China
关键词:
筛选食用真菌木质纤维素降解
Keywords:
screeningedible fungilignocellulolsedegradation
分类号:
Q815
DOI:
10.7685/j.issn.1000-2030.2012.06.009
摘要:
以常见的7株食用真菌菌株为材料,分别为平菇韩黑(Pleurotus ostreatus hanhei)和平菇8号(P.ostreatus 8)、秀珍菇(P.cornucopiae)、姬菇206(Hypsizygus marmoreus 206)、白灵菇(Pleurotus nebrodensis)、金针菇天福(Flammulina velutipes tianfu)和金针菇8802(F.velutipes 8802),筛选出在纤维素平板上生长较快的4株菌(姬菇206、平菇韩黑、平菇8号和秀珍菇),并进一步测定其产生纤维素酶、半纤维素酶和木质素酶的活力以及降解稻草中木质素、纤维素和半纤维素的能力。结果显示:姬菇206中与木质素降解有关的木质素过氧化物酶(LiPs)、锰依赖过氧化物酶(MnPs)和漆酶(Lacs)的活力最大,分别达到58.52、186.66和8.82 U·g-1;滤纸酶活力也是姬菇206最高,为3.86 U·mL-1;平菇韩黑的半纤维素酶活力最高,达到41.20 U·mL-1。稻草降解试验表明,平菇韩黑的半纤维素降解率最高,为51.03%,其次为姬菇206,降解率为50.49%。姬菇206对木质素的分解率最高,达到51.47%。平菇韩黑对稻草的的总降解率最高,达到47.52%。结论:平菇韩黑和姬菇206具有较强的稻草降解能力,可作为降解稻草的工程菌株。
Abstract:
In this study,seven edible fungi were chosen for detecting their abilities of lignin,cellulose and hemicellulose degradation.Four edible fungi which were Pleurotus ostreatus hanhei,P.ostreatus 8,P.cornucopiae,and Hypsizygus marmoreus 206 grew faster on the media with cellulose as the sole carbon source than others.The relative enzyme profiles of the four fungi were examined.The results showed that the fungal H.marmoreus 206 had maximum activities of lignin degrading enzymes,and the values were 58.52 U·g-1,186.66 U·g-1 and 8.82 U·g-1,respectively,for LiPs,MnPs and Lacs.H.marmoreus 206 also produced the maximal filter paper enzyme activity,3.86 U·mL-1.The highest activity of xylanase was 41.20 U·mL-1 on the 16 day by P.ostreatus hanhei.During the 18 days fermentation,P.ostreatus hanhei had the highest hemicellulose degradation rate(51.03%),and the second was H.marmoreus 206(50.49%).While H.marmoreus 206 had the highest lignin degradation rate(51.47%).All in all,P.ostreatus hanhei displayed the maximum rice straw degradation rate(47.52%),compared to that of H.marmoreus 206(41.80%),P.ostreatus 8(36.00%),P.cornucopiae(35.63%).It could be concluded that P.ostreatus hanhei and H.marmoreus 206 showed the higher straw degradation abilities and could be regarded as potential straw degradation fungi.

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备注/Memo

备注/Memo:
收稿日期:2011-12-2;改回日期:。
作者简介:孙江慧,硕士研究生。
通讯作者:沈标,教授,研究方向为环境微生物学,E-mail:shenbiao@njau.edu.cn。
更新日期/Last Update: 1900-01-01