[1]郭凡溪,刘腾飞,耿智霞,等.复方甘草酸单铵可溶性粉对恩诺沙星联合LPS致鸡肝损伤保护机制研究[J].南京农业大学学报,2013,36(2):115-120.[doi:10.7685/j.issn.1000-2030.2013.02.019]
 GUO Fanxi,LIU Tengfei,GENG Zhixia,et al.Protective effects of compound ammonium glycyrrhizin soluble powder on liver injury induced by LPS and enrofloxacin in chickens[J].Journal of Nanjing Agricultural University,2013,36(2):115-120.[doi:10.7685/j.issn.1000-2030.2013.02.019]
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复方甘草酸单铵可溶性粉对恩诺沙星联合LPS致鸡肝损伤保护机制研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年2期
页码:
115-120
栏目:
OA栏目
出版日期:
2013-03-22

文章信息/Info

Title:
Protective effects of compound ammonium glycyrrhizin soluble powder on liver injury induced by LPS and enrofloxacin in chickens
作者:
郭凡溪 刘腾飞 耿智霞 蒋凡 余祖功
南京农业大学动物医学院, 江苏 南京 210095
Author(s):
GUO Fanxi LIU Tengfei GENG Zhixia JIANG Fan YU Zugong
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
关键词:
复方甘草酸单铵可溶性粉复合式肝损伤恩诺沙星LPS自由基
Keywords:
compound ammonium glycyrrhizin soluble powdercomplex liver injuryenrofloxacinLPSfree radicalschicken
分类号:
S859.7
DOI:
10.7685/j.issn.1000-2030.2013.02.019
摘要:
104羽海蓝蛋鸡随机分为空白组(Ⅰ)、模型组(Ⅱ)、复方甘草酸单铵可溶性粉(CAG)预防组(Ⅲ)、CAG治疗高剂量组(Ⅳ)和CAG治疗低剂量组(Ⅴ),除Ⅰ组外,各组连续灌服恩诺沙星100 mg·kg-1,1次·d-1,第3天灌药同时腹腔注射脂多糖粗提液(LPS)4 mL·kg-1以建模。Ⅲ组在灌服恩诺沙星前3 d混合饮用CAG 40 mg·L-1至建模止;Ⅳ、Ⅴ组腹腔注射LPS同时分别灌服22.5和7.5 mg·kg-1 CAG,2次·d-1,重复3 d。各组均在腹腔注射LPS后分别于6、24和48 h采血,检测血清生化指标,观察肝组织病理学变化。试验结果如下:Ⅱ组鸡血清ALT、AST、γ-GT、TBIL、MDA和NO水平在6、24和48 h均显著高于Ⅰ组,而TP、ALB、SOD和GSH均显著低于Ⅰ组。与Ⅱ组相比,Ⅲ组各指标多在6和24 h差异显著,只MDA、ALB和GSH持续至48 h仍显著,Ⅳ组各指标均在24和48 h差异显著,Ⅴ组各指标差异均不显著。仅Ⅳ组DBIL浓度在24和48 h较Ⅰ组显著升高,其余组无显著差异;Ⅳ组较其他组鸡肝细胞病变改善最大。结论:在自由基损伤介导肝损伤过程中,CAG有一定保护作用,效果受给药时机和剂量影响。
Abstract:
A total of 104 chickens was divided randomly into 5 groups:GroupⅠserved as normal control.GroupⅡ,Ⅲ,Ⅳ,andⅤwere administered enrofloxacin(100 mg·kg-1)once a day,and on the 3rd day,administered lipopolysaccharide crude extract(LPS)(4 mL·kg-1)at the same time.GroupⅡwas maintained as liver injury control.Group Ⅲ received preventive treatment of compound ammonium glycyrrhizin soluble powder(CAG)in drinking water at the dose of 40 mg·L-1(by ammonium glycyrrhizin,the same as follows)for three days before enrofloxacin treatment,and till to LPS treatment.Group Ⅳ andⅤreceived CAG at the dose of 22.5 and 7.5 mg·kg-1,twice a day for three days after LPS treatment.Eight chickens were sacrificed at 6,24,48 h post-treatment with LPS and their blood sample were harvested for detection of serum biochemical indexes.Their livers were dissected out and evaluated for histopathological changes.Result:at 6,24,48 h post-treatment with LPS,the levels of serum ALT,AST,γ-GT,TBIL,MDA and NO were significantly higher,and TP,ALB,SOD and GSH were significantly lower in Group Ⅱ than in GroupⅠ.Compared to Group Ⅱ,in Group Ⅲ,there was significant improvment of most indicators at 6 h and 24 h,but MDA,ALB and GSH at 6,24 and 48 h;in group Ⅳ,there was significantly improvment of all indicators at 24 h and 48 h.There was no obvious alteration were still significantly higher in Group Ⅴ.There was significantly higher improvement in DBIL at 24 h and 48 h in Group Ⅳ than in GroupⅠ,no obvious alteration in others.The results of pathological study also support the results of biochemical findings.Conclusion:the liver injury effect might be related to oxygen free radicals.CAG treatment could reduce such liver injury,probably by its antioxidant property.

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备注/Memo

备注/Memo:
收稿日期:2012-11-28;改回日期:。
基金项目:国家自然科学基金项目(31172282)
作者简介:郭凡溪,硕士研究生。
通讯作者:余祖功,副教授,主要从事新兽药药理研究及兽药新制剂的研发,E-mail:yuzugong@njau.edu.cn。
通讯作者:余祖功,副教授,主要从事新兽药药理研究及兽药新制剂的研发,E-mail:yuzugong@njau.edu.cn。
更新日期/Last Update: 1900-01-01