[1]胡雲飞,张玥,张彩丽,等.茶树根系内生真菌与根际土壤真菌的季节多样性分析[J].南京农业大学学报,2013,36(3):41-46.[doi:10.7685/j.issn.1000-2030.2013.03.007]
 HU Yunfei,ZHANG Yue,ZHANG Caili,et al.Diversity analysis of the endophytic fungi in root and fungi in rhizosphere soil of tea plant in different seasons[J].Journal of Nanjing Agricultural University,2013,36(3):41-46.[doi:10.7685/j.issn.1000-2030.2013.03.007]
点击复制

茶树根系内生真菌与根际土壤真菌的季节多样性分析()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年3期
页码:
41-46
栏目:
出版日期:
2013-05-30

文章信息/Info

Title:
Diversity analysis of the endophytic fungi in root and fungi in rhizosphere soil of tea plant in different seasons
作者:
胡雲飞12 张玥12 张彩丽1 杨路成1 陈暄1 庄静1 黎星辉12
1. 南京农业大学茶叶科学研究所, 江苏 南京 210095;
2. 江苏省茶学研究生工作站, 江苏 苏州 215128
Author(s):
HU Yunfei12 ZHANG Yue12 ZHANG Caili1 YANG Lucheng1 CHEN Xuan1 ZHUANG Jing1 LI Xinghui12
1. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
2. The Graduate Research Station for Tea Science of Jiangsu Province, Suzhou 215128, China
关键词:
根际土壤根系真菌茶树多样性指数
Keywords:
rhizosphere soilrootfungusCamellia sinensisdiversity index
分类号:
S571.1
DOI:
10.7685/j.issn.1000-2030.2013.03.007
摘要:
从不同季节的茶树根系和根际土壤中分离得到真菌2 019株,包括茶树根系内生真菌826株、根际土壤真菌1 193株。采用分子生物学鉴定方法,对rDNA-ITS区段进行扩增及分析,共鉴定出13个属28种真菌,其中茶树根系内生真菌12种,根际土壤真菌20种。对不同季节分离得到的真菌进行多样性分析,结果表明:春、夏、秋季分离出茶树根系内生真菌种类分别为12、11、12;3个季节Shannon-Wiener多样性指数差异不明显;而均匀度指数夏季最高,秋季次之,春季最低。春、夏、秋季分离出根际土壤真菌种类分别为18、17、20;3个季节Shannon-Wiener多样性指数及均匀度指数差异均不明显。根际土壤真菌与根系内生真菌比较,春、秋两季Shannon-Wiener多样性指数和均匀度指数差异明显且根际土壤真菌高于根系内生真菌;夏季两者差异均不明显。
Abstract:
A total of 2 019 fungi were isolated from root endophyte and rhizosphere soil of tea plant(Camellia sinensis(L.)O.Kuntze)in different seasons,including 826 endophytic fungi and 1 193 fungi in the rhizosphere soil.The amplification and analysis of rDNA-ITS sequence of the fungi revealed that a total of 28 species of 13 genera of fungi were identified,which includes 12 species of endophytic fungi and 20 species of fungi in the rhizosphere soil.The diversity analysis result of the fungi isolated from different seasons indicated that the difference of Shannon-Wiener diversity index was insignificant among endophytic fungi from three seasons,including 12 species from spring,11 species from summer and 12 species from autumn,while evenness index from summer was the highest,followed by autumn and spring.Species of fungi in the rhizosphere soil from spring,summer and autumn were 18,17 and 20,respectively.Shannon-Wiener diversity index and evenness index of fungi in the rhizosphere soil had no significant difference.Compared to the root endophytic fungi,Shannon-Wiener diversity index and evenness index of fungi in the rhizosphere soil in spring and autumn had significant difference and were higher,but both had no significant difference in summer.

参考文献/References:

[1] 伍丽,余有本,周天山,等.茶树根际土壤因子对根际微生物数量的影响[J].西北农业学报,2011,20(4):159-163
[2] 梁慧玲,董尚胜.茶园土壤微生物研究现状[J].茶叶,2001,27(4):3-5
[3] 李远华,郑芳,倪德江,等.茶树接种VA菌根的生理特性研究[J].茶叶科学,2011,31(6):504-512
[4] 吴丽莎,王玉,赵青华,等.丛枝菌根真菌对实生茶树叶片光合性能的影响[J].青岛农业大学学报:自然科学版,2011,28(1):13-15
[5] 孙海新,刘训理.茶树根际微生物研究[J].生态学报,2004,24(7):1353-1357
[6] 仝瑞建,刘雪琴,王发园,等.沙田柚根围AM真菌的生境适宜性和季节变化性[J].应用与环境生物学报,2011,17(5):684-687
[7] 罗佳,赵爽,袁玉娟,等.施用微生物有机肥对棉花抗病性相关酶活性的影响[J].南京农业大学学报,2011,34(3):89-93.doi:10.7685/j.issn.1000-2030.2011.03.016
[8] 刘红艳,邓欣.我国茶树根际微生物研究现状及展望[J].茶叶通讯,2003(4):14-18
[9] Brundrett M C.Understanding the roles of multifunctional mycorrhizal and endophytic fungi[M]//Schulz B,Boyle C,Sieber T N.Berlin Microbial Root Endophytes.Heidelberg:Springer-Verlag,2006:281-293
[10] Baltruschat H,Fodor J,Harrach B D,et al.Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants[J].New Phytol,2008,180:501-510
[11] 陈晖奇,徐焰平,谢丽华,等.茶树内生真菌的分离及其在寄主组织中的分布特征[J].莱阳农学院学报,2006,23(4):250-254
[12] Morakotkarn D,Kawasaki H,Seki T.Molecular diversity of bamboo-associated fungi isolated from Japan[J].Fems Microbiology Letters,2007,266:10-19
[13] 李阜棣,喻子牛,何绍江.农业微生物学实验技术[M].北京:中国农业出版社,1996:62-63
[14] Bartlett J M S,Stirling D.DNA extraction from fungi,yeast,and bacteria[J].Methods Mol Biol,2003,226:53-54
[15] White T J,Bruns T,Lee S,et al.Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics[M].New York:Academic Press,1990:315-322
[16] Tamura K,Dudley J,Nei M,et al.MEGA4:molecular evolutionary genetics analysis(MEGA)software version 4.0[J].Molecular Biology and Evolution,2007,24:1596-1599
[17] Tejesvi M V,Ruotsalainen A L,Markkola A M,et al.Root endophytes along a primary succession gradient in northern Finland[J].Fungal Diversity,2010,41(1):125-134
[18] 孙剑秋,郭良栋,臧威,等.药用植物内生真菌多样性及生态分布[J].中国科学C辑:生命科学,2008,38(5):475-484
[19] Lucia S,Cornelis A M V G,Annamaria R,et al.Soil invertebrates as bioindicators of urban soil quality[J].Environmental Pollution,2012,161:57-63
[20] Rodriguez R J,White J F,Arnold A E,et al.Fungal endophytes:diversity and functional roles[J].New Phytol,2009,182:314-330
[21] Shipra S,Anita P,Bhavesh K,et al.Enhancement in growth and quality parameters of teathrough inoculation with arbuscular mycorrhizal fungi in an acid soil[J].Biol Fertil Soils,2010,46:427-433
[22] Xue D,Huang X D,Yao H Y,et al.Effect of lime application on microbial community in acidic tea orchard soils in comparison with those in wasteland and forest soils[J].Journal of Environmental Sciences,2010,22(8):1253-1260
[23] 蔡燕飞,廖宗文,章家恩,等.生态有机肥对番茄青枯病及土壤微生物多样性的影响[J].应用生态学报,2003,14(3):349-353
[24] 田朝光,马延和.真菌降解木质纤维素的功能基因组学研究进展[J].生物工程学报,2010,26(10):1333-1339
[25] 张铎,解莉,张丽萍,等.棉花黄萎病生物防治研究[J].安徽农业科学,2007,35(11):3302-3303,3357
[26] 王世强,胡长玉,程东华,等.Al3+胁迫对茶园土壤微生物区系及生理群的影响[J].环境污染与防治,2011,33(6):36-43
[27] 葛德永,姚槐应,黄昌勇.茶园土壤耐酸铝微生物的分离鉴定及其耐铝特性研究[J].浙江大学学报:农业与生命科学版,2007,33(6):626-632

相似文献/References:

[1]陈艳,乔璟,沈益新*.春季紫花苜蓿根系性状与地上部生长性状的相关性分析[J].南京农业大学学报,2012,35(1):108.[doi:10.7685/j.issn.1000-2030.2012.01.019]
 CHEN Yan,QIAO Jing,SHEN Yi-xin*.Analysis of the relationship between root traits and plant growth traits of alfalfa in spring[J].Journal of Nanjing Agricultural University,2012,35(3):108.[doi:10.7685/j.issn.1000-2030.2012.01.019]
[2]张鹏,韦中,朱震,等.生物有机肥对连作番茄和辣椒根际土壤微生物区系及茄科雷尔氏菌的影响[J].南京农业大学学报,2013,36(4):77.[doi:10.7685/j.issn.1000-2030.2013.04.013]
 ZHANG Peng,WEI Zhong,ZHU Zhen,et al.Effect of a bio-organic fertilizer on microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper[J].Journal of Nanjing Agricultural University,2013,36(3):77.[doi:10.7685/j.issn.1000-2030.2013.04.013]
[3]陈鹏,张茂星,张明超,等.不同氮素营养形态对香蕉生长及其根系质子泵活性的影响[J].南京农业大学学报,2015,38(1):101.[doi:10.7685/j.issn.1000-2030.2015.01.015]
 CHEN Peng,ZHANG Maoxing,ZHANG Mingchao,et al.Influence of nitrate/ammonium ratio on the growth and root plasma membrane H+-ATPase activity of banana[J].Journal of Nanjing Agricultural University,2015,38(3):101.[doi:10.7685/j.issn.1000-2030.2015.01.015]
[4]李春艳,李思忠,楚光红,等.不同滴水量处理下大豆根系生长与花荚形成的关系[J].南京农业大学学报,2016,39(2):198.[doi:10.7685/jnau.201509024]
 LI Chunyan,LI Sizhong,CHU Guanghong,et al.Relationship of soybean root growth and flower-pod formation under different irrigation quantities[J].Journal of Nanjing Agricultural University,2016,39(3):198.[doi:10.7685/jnau.201509024]
[5]王英珍,张虎平,黄小三,等.钾在梨树内的分配及对梨树生长和叶片光合能力的影响[J].南京农业大学学报,2017,40(1):60.[doi:10.7685/jnau.201603054]
 WANG Yingzhen,ZHANG Huping,HUANG Xiaosan,et al.Effect of potassium supply on plant potassium distribution and growth and leaf photosynthetic capacity of Pyrus pyrifolia[J].Journal of Nanjing Agricultural University,2017,40(3):60.[doi:10.7685/jnau.201603054]

备注/Memo

备注/Memo:
收稿日期:2012-5-8;改回日期:。
基金项目:现代农业产业技术体系建设专项资金(CARS-23);国家自然科学基金项目(31000315);江苏高校优势学科建设工程专项资金;江苏省科技支撑计划项目(BE2011319);苏州市科技发展计划项目(SZGD201067,WNZ1002,吴政抄2009字65号)
作者简介:胡雲飞,硕士研究生。
通讯作者:黎星辉,博士,教授,主要从事茶树栽培与育种研究,E-mail:lxh@njau.edu.cn。
更新日期/Last Update: 1900-01-01