[1]张鹏,韦中,朱震,等.生物有机肥对连作番茄和辣椒根际土壤微生物区系及茄科雷尔氏菌的影响[J].南京农业大学学报,2013,36(4):77-82.[doi:10.7685/j.issn.1000-2030.2013.04.013]
 ZHANG Peng,WEI Zhong,ZHU Zhen,et al.Effect of a bio-organic fertilizer on microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper[J].Journal of Nanjing Agricultural University,2013,36(4):77-82.[doi:10.7685/j.issn.1000-2030.2013.04.013]
点击复制

生物有机肥对连作番茄和辣椒根际土壤微生物区系及茄科雷尔氏菌的影响()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年4期
页码:
77-82
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Effect of a bio-organic fertilizer on microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper
作者:
张鹏 韦中 朱震 高雪莲 邓开英 冉炜 沈其荣
南京农业大学江苏省固体有机废弃物资源化高技术研究重点实验室, 江苏 南京 210095
Author(s):
ZHANG Peng WEI Zhong ZHU Zhen GAO Xuelian DENG Kaiying RAN Wei SHEN Qirong
Jiangsu Key Laboratory of Soild Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China
关键词:
生物有机肥根际土壤微生物区系青枯病番茄辣椒
Keywords:
bio-organic fertilizerrhizosphere soilmicroflorabacterial wilttomatopepper
分类号:
S144
DOI:
10.7685/j.issn.1000-2030.2013.04.013
摘要:
利用平板计数、实时荧光定量RT-PCR和PCR-DGGE指纹图谱等方法研究田间条件下连作番茄和辣椒施用生物有机肥(BOF)和常规施肥(CK)的根际土壤微生物组成差异。结果表明:与CK相比,BOF处理的番茄和辣椒产量分别提高了26.0%和19.9%,青枯病发病率分别降低了41.5%和44.7%;对于未发病植株,与CK相比,BOF处理的番茄和辣椒植株根际土壤细菌数量分别增加了62.7%和142.4%,放线菌数量分别增加了32.8%和39.6%,真菌数量分别减少了43.9%和53.1%,病原菌数量分别减少了73.2%和90.1%;对于发病植株,与CK相比,BOF处理的番茄和辣椒植株根际土壤细菌和放线菌数量没有显著差异,但真菌和病原菌数量显著降低。PCR-DGGE分析表明,施用BOF提高了番茄和辣椒植株根际土壤微生物群落结构多样性指数、丰富度指数和稳定性指数。
Abstract:
Microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper in field under application of a bio-organic fertilizer(BOF)and conventional management(CK)were studied with focus on the difference of microflora in rhizosphere soil of tomato and pepper by using plate count,real-time quantitative PCR and PCR-DGGE fingerprint techniques.As compared with CK,the BOF treatment increased the yields of tomato and pepper by 26.0% and 19.9% and decreased the bacterial wilt incidence rates of tomato and pepper by 41.5% and 44.7%,respectively.For non-diseased plants,the BOF treatment increased the numbers of bacteria and actinomycetes by 62.7% and 32.8% in rhizosphere soils of tomato and by 142.4% and 39.6% in rhizosphere soils of pepper,and decreased the numbers of fungi and R.solanacearum by 43.9% and 73.2% in rhizosphere soils of tomato and by 53.1% and 90.1% in rhizosphere soils of pepper,respectively,as compared with CK.For diseased plants,no significant difference between the BOF treatment and CK existed in the numbers of bacteria and actinomycetes in rhizosphere soils of both tomato and pepper,but the BOF treatment significantly decreased the numbers of fungi and R.solanacearum in rhizosphere soils of both tomato and pepper as compared with CK.PCR-DGGE analysis showed that application of BOF enhanced the diversity,richness and stability indices of microbial community in the rhizosphere soils of tomato and pepper.

参考文献/References:

[1] Hayward A C.Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum[J].Annu Rev Phytopathol,1991,29:65-87
[2] He L Y,Sequeira L,Kelman A.Characteristics of strains of Pseudomonas solanacearum from China[J].Plant Disease,1983,67(12):1357-1361
[3] Hoitink H A J,Boehm M J.Biocontrol within the context of soil microbial communities:a substrate-dependent phenomenon[J].Annu Rev Phytopathol,1999,37:427-446
[4] Wei Z,Shen Q R,Yin S,et al.Efficacy of Bacillus-fortified organic fertiliser in controlling bacterial wilt of tomato in the field[J].Appl Soil Ecol,2011,48:152-159
[5] 江欢欢,程凯,沈其荣,等.辣椒青枯病拮抗菌的筛选及其生物防治效应[J].土壤学报,2010,47(6):1225-1231
[6] 蔡燕飞,廖宗文,章家恩,等.生态有机肥对蕃茄青枯病及土壤微生物多样性的研究[J].应用生态学报,2003,14(3):349-353
[7] Bowen G D,Rovira A D.The rhizosphere and its management to improve plant growth[J].Advances in Agronomy,1999,66:1-102
[8] Farrar J,Haes M,Jones D,et al.How roots control the flux of carbon to the rhizosphere[J].Ecology,2003,84:827-837
[9] 朱震,陈芳,沈其荣,等.拮抗菌生物有机肥对番茄根结线虫的防治作用[J].应用生态学报,2011,22(4):1033-1038
[10] 范秀容,沈萍,李广武.微生物学实验[M].3版.北京:高等教育出版社,1999:67-72
[11] Schönfeld J,Heuer H,van Elsas J D,et al.Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments[J].Applied and Environmental Microbiology,2003,69(12):7248-7256
[12] 萨姆布鲁克 J,拉斐尔 D W.分子克隆实验指南[M].黄培堂,王恒樑,周晓巍,等译.3版.北京:科学出版社,2002:27-105
[13] 罗佳.微生物有机肥对棉花黄萎病的生物防治及其作用机制[D].南京:南京农业大学,2010
[14] May L A,Smiley B,Schmidt M G.Comparative denaturing gradient gel electrophoresis analysis of fungal communities associated with whole plant corn silage[J].Can J Microbiol,2001,47:829-841
[15] Asakawa S,Kimura M.Comparison of bacterial community structures at main habitats in paddy field ecosystem based on DGGE analysis[J].Soil Biology and Biochemistry,2008,40(6):1322-1329
[16] 凌宁,王秋君,沈其荣,等.根际施用微生物有机肥防治连作西瓜枯萎病研究[J].植物营养与肥料学报,2009,15(5):1136-1141
[17`s\乿l VE`s\乿l Vt al.Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium dahliae Kleb.[J].Applied and Environmental Microbiology,2005,71(8):4203-4213
[18] Garmendia I,Aguirreolea J,Goicoechea N.Defence-related enzymes in pepper roots during interactions with arbuscular mycorrhizal fungi and/or Verticillium dahliae[J].BioControl,2006,51:293-310
[19] Müller H,Berg G.Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape[J].BioControl,2008,53(6):905-916
[20] 陈巧玲,胡江,汪汉成,等.生物有机肥对盆栽烟草根际青枯病原菌和短短芽孢杆菌数量的影响[J].南京农业大学学报,2012,35(1):75-79.doi:10.7685/j.issn.1000-2030.2012.01.013
[21] 薛超,黄启为,沈其荣,等.连作土壤微生物区系分析、调控及高通量研究方法[J].土壤学报,2011,48(3):612-618
[22] 王超,吴凡,刘训理,等.不同肥力条件下烟草根际微生物的初步研究[J].中国烟草科学,2005(2):12-14
[23] 张慧.防治棉花黄萎病微生物有机肥的研制及其生物效应.南京:南京农业大学,2008
[24] Gorissen A,van Overbeek L S,van Elsas J D.Pig slurry reduces the survival of Ralstonia solanacearum biovar 2 in soil[J].Canadian Journal of Microbiology,2004,50(8):587-593
[25] Marschner P,Yang C H,Lieberei R,et al.Soil and plant specific effects on bacterial community composition in the rhizosphere[J].Soil Biol Biochem,2001,33:1437-1445
[26] Marscher P,Neumann G,Kania A,et al.Spatial and temporal dynamics of the microbial community structure in the rhizosphere of cluster roots of white lupin(Lupinus albus L.)[J].Plant and Soil,2002,246(2):167-174

相似文献/References:

[1]陈巧玲,胡江*,汪汉成,等.生物有机肥对盆栽烟草根际青枯病原菌和短短芽孢杆菌数量的影响[J].南京农业大学学报,2012,35(1):75.[doi:10.7685/j.issn.1000-2030.2012.01.013]
 CHEN Qiao-ling,HU Jiang*,WANG Han-cheng,et al.Effects of bio-organic fertilizer application on population of Ralstonia solanacearum and Brevibacillus brevis in tobacco rhizosphere[J].Journal of Nanjing Agricultural University,2012,35(4):75.[doi:10.7685/j.issn.1000-2030.2012.01.013]
[2]高雪莲,邓开英,张鹏,等.不同生物有机肥对甜瓜土传枯萎病防治效果及对根际土壤微生物区系的影响[J].南京农业大学学报,2012,35(6):55.[doi:10.7685/j.issn.1000-2030.2012.06.010]
 GAO Xue-lian,DENG Kai-ying,ZHANG Peng,et al.Effects of different bio-organic fertilizers on fusarium wilt control and on the rhizosphere microbial community with muskmelon[J].Journal of Nanjing Agricultural University,2012,35(4):55.[doi:10.7685/j.issn.1000-2030.2012.06.010]
[3]张国漪,丁传雨,任丽轩,等.菌根真菌和死谷芽孢杆菌生物有机肥对连作棉花黄萎病的协同抑制[J].南京农业大学学报,2012,35(6):68.[doi:10.7685/j.issn.1000-2030.2012.06.012]
 ZHANG Guo-yi,DING Chuan-yu,REN Li-xuan,et al.The synergetic inhibition effect of arbuscular mycorrhizal fungi cooperated Bacillus vallismortis organic fertilizer on cotton Verticillium wilt[J].Journal of Nanjing Agricultural University,2012,35(4):68.[doi:10.7685/j.issn.1000-2030.2012.06.012]
[4]邓开英,凌宁,张鹏,等.专用生物有机肥对营养钵西瓜苗生长和根际微生物区系的影响[J].南京农业大学学报,2013,36(2):103.[doi:10.7685/j.issn.1000-2030.2013.02.017]
 DENG Kaiying,LING Ning,ZHANG Peng,et al.Effects of bio-organic fertilizers on the growth and rhizosphere microflora of watermelon plants in nursery pots[J].Journal of Nanjing Agricultural University,2013,36(4):103.[doi:10.7685/j.issn.1000-2030.2013.02.017]
[5]胡雲飞,张玥,张彩丽,等.茶树根系内生真菌与根际土壤真菌的季节多样性分析[J].南京农业大学学报,2013,36(3):41.[doi:10.7685/j.issn.1000-2030.2013.03.007]
 HU Yunfei,ZHANG Yue,ZHANG Caili,et al.Diversity analysis of the endophytic fungi in root and fungi in rhizosphere soil of tea plant in different seasons[J].Journal of Nanjing Agricultural University,2013,36(4):41.[doi:10.7685/j.issn.1000-2030.2013.03.007]
[6]马田田,蔡枫,丁传雨,等.山药专用生物有机肥的生物效应研究[J].南京农业大学学报,2013,36(4):83.[doi:10.7685/j.issn.1000-2030.2013.04.014]
 MA Tiantian,CAI Feng,DING Chuanyu,et al.Biological effects of special bio-organic fertilizer on yam[J].Journal of Nanjing Agricultural University,2013,36(4):83.[doi:10.7685/j.issn.1000-2030.2013.04.014]
[7]曹亮亮,张苗,施娟娟,等.添加蛋白原料辅助固态发酵生产功能菌生物有机肥的研究[J].南京农业大学学报,2014,37(2):85.[doi:10.7685/j.issn.1000-2030.2014.02.014]
 CAO Liangliang,ZHANG Miao,SHI Juanjuan,et al.Adding protein materials as solid-state fermentation media for producing bio-organic fertilizers with functional microbes[J].Journal of Nanjing Agricultural University,2014,37(4):85.[doi:10.7685/j.issn.1000-2030.2014.02.014]
[8]陈希,赵爽,史亚东,等.生物有机肥对‘滁菊’连作障碍的影响[J].南京农业大学学报,2015,38(1):50.[doi:10.7685/j.issn.1000-2030.2015.01.008]
 CHEN Xi,ZHAO Shuang,SHI Yadong,et al.Effects of bio-organic fertilizer application on the continuous cropping obstacles of Chrysanthemum morifolium Tzvel.‘Chuju’[J].Journal of Nanjing Agricultural University,2015,38(4):50.[doi:10.7685/j.issn.1000-2030.2015.01.008]
[9]韦中,胡洁,董月,等.基于菜粕有机肥筛选番茄青枯病高效生防菌的研究[J].南京农业大学学报,2015,38(3):424.[doi:10.7685/j.issn.1000-2030.2015.03.011]
 WEI Zhong,HU Jie,DONG Yue,et al.Screen of antagonists against Ralstonia solanacearum by using rapeseed cake compost as a selected medium[J].Journal of Nanjing Agricultural University,2015,38(4):424.[doi:10.7685/j.issn.1000-2030.2015.03.011]
[10]巩子毓,高旭,黄炎,等.连续施用生物有机肥提高设施黄瓜产量和品质的研究[J].南京农业大学学报,2016,39(5):777.[doi:10.7685/jnau.201602020]
 GONG Ziyu,GAO Xu,HUANG Yan,et al.Research on continuous application of bio-organic fertilizer for improving greenhouse cucumber yield and quality[J].Journal of Nanjing Agricultural University,2016,39(4):777.[doi:10.7685/jnau.201602020]

备注/Memo

备注/Memo:
收稿日期:2012-4-28;改回日期:。
基金项目:广东省教育厅产学研结合项目(2010B090400431);江苏省科技支撑计划项目(BE2009347)
作者简介:张鹏,硕士研究生。
通讯作者:冉炜,研究员,博士,研究方向为生物有机肥,E-mail:ranwei@njau.edu.cn。
更新日期/Last Update: 1900-01-01