[1]黄金虎,刘民星,商可心,等.46株猪链球菌对大环内酯类抗生素的耐药性及PFGE分型[J].南京农业大学学报,2013,36(4):105-110.[doi:10.7685/j.issn.1000-2030.2013.04.017]
 HUANG Jinhu,LIU Minxing,SHANG Kexin,et al.Macrolide resistance and pulsed-field gel electrophoresis typing of 46 Streptococcus suis isolates[J].Journal of Nanjing Agricultural University,2013,36(4):105-110.[doi:10.7685/j.issn.1000-2030.2013.04.017]
点击复制

46株猪链球菌对大环内酯类抗生素的耐药性及PFGE分型()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年4期
页码:
105-110
栏目:
出版日期:
2013-07-15

文章信息/Info

Title:
Macrolide resistance and pulsed-field gel electrophoresis typing of 46 Streptococcus suis isolates
作者:
黄金虎 刘民星 商可心 王丽平
南京农业大学动物医学院, 江苏 南京 210095
Author(s):
HUANG Jinhu LIU Minxing SHANG Kexin WANG Liping
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
关键词:
猪链球菌ermBmefA耐药性脉冲场凝胶电泳水平传播
Keywords:
Streptococcus suisermBmefAresistancePFGEhorizontal transmission
分类号:
S859.7
DOI:
10.7685/j.issn.1000-2030.2013.04.017
摘要:
参照CLSI推荐的肉汤微量稀释法检测了46株猪链球菌对8种抗生素的耐药性,采用PCR方法检测其大环内酯类耐药基因的分布情况,并用脉冲场凝胶电泳(PFGE)分型方法分析大环内酯类抗生素耐药株和敏感株的遗传差异性。试验结果显示:46株猪链球菌对四环素的耐药率达到100%,对头孢喹肟、红霉素、泰乐菌素、替米考星、阿奇霉素和氨苄西林的耐药率分别为43.48%、34.78%、26.09%、23.91%、15.22%和2.17%,所有猪链球菌对氟苯尼考表现为敏感或中介。46株猪链球菌中有34.78%(16/46)的菌株携带大环内酯类耐药基因,均表现出对大环内酯类抗生素耐药。其中12株携带ermB基因,菌株为MLSB耐药表型,是大环内酯类耐药的主要方式;4株携带mefA基因,菌株为M型耐药表型。PFGE指纹图谱显示16株ermBmefA耐药株的图谱不一致,存在明显的遗传差异性。结果提示猪链球菌对抗生素的耐药性较高,大环内酯类耐药基因在猪链球菌中广泛存在,其中以ermB介导的核糖体23S rRNA甲基化占主导地位,其次是mefA介导的药物外排系统。这些耐药菌株分属不同的克隆株,推测耐药基因ermB可能通过移动基因元件的水平转移而广泛传播。
Abstract:
The aim of the study is to analyse the macrolides resistant rate and the genetic diversity of Streptococcus suis clinical isolates as well as the possible mode of the transmission of macrolide resistance genes.The broth microdilution method was used to detect the antimicrobial sensitivity of eight antibiotics against 46 S.suis.PCR was used to detect the macrolide resistance genes ermA/B/C,mefA and msrD.And genotyping was carried out by pulsed-field gel electrophoresis(PFGE).The results showed that S.suis was resistant to tetracycline,cefquinome,erythromycin,azithromycin,tylosin,tilmicosin and ampicillin with the different rate of 100%,43.48%,34.78%,15.22%,26.09%,23.91% and 2.17%,respectively.Fourty-six strains were not resistant to florfenicol.Macrolide resistance gene ermB was detected in 12 strains and mefA was found in 4 strains.PFGE typing result implied that the isolates represented a wide variety of genetic difference.The study indicates that there is a high resistant rate to macrolide due to the acquirement of ermB and mefA resistant genes in S.suis.The genomic polymorphism of the macrolide resistant strains is more apparent than that of the sensitive strains,which indicates the possible transmission mode of the resistance is horizontal way,but not clone spread.

参考文献/References:

[1] Higgins R,Gottschalk M.Streptococcal diseases[M]//D’Allaire S,Taylor D J,Zimmerman J.Diseases of Swine.9th ed.Ames:Blackwell Publishing,2006:769-783
[2] Staats J J,Feder I,Okwumabua O,et al.Streptococcus suis:past and present[J].Vet Res Commun,1997,21(6):381-407
[3] Marie J,Morvan H,Berthelot-Herault F,et al.Antimicrobial susceptibility of Streptococcus suis isolated from swine in France and from humans in different countries between 1996 and 2000[J].J Antimicrob Chemother,2002,50(2):201-209
[4] Jumbe N L,Louie A,Miller M H,et al.Quinolone efflux pumps play a central role in emergence of fluoroquinolone resistance in Streptococcus pneumoniae[J].Antimicrob Agents Chemother,2006,50(1):310-317
[5] Hu P,Yang M,Zhang A,et al.Comparative genomics study of multi-drug-resistance mechanisms in the antibiotic-resistant Streptococcus suis R61 strain[J].PLoS One,2011,6(9):e24988
[6] Martel A,Devriese L A,Decostere A,et al.Presence of macrolide resistance genes in Streptococci and Enterococci isolated from pigs and pork carcasses[J].Int J Food Microbiol,2003,84(1):27-32
[7] Brenciani A,Bacciaglia A,Vecchi M,et al.Genetic elements carrying erm(B)in Streptococcus pyogenes and association with tet(M)tetracycline resistance gene[J].Antimicrob Agents Chemother,2007,51(4):1209-1216
[8] Palmieri C,Princivalli M S,Brenciani A,et al.Different genetic elements carrying the tet(W)gene in two human clinical isolates of Streptococcus suis[J].Antimicrob Agents Chemother,2011,55(2):631-636
[9] Brenciani A,Bacciaglia A,Vignaroli C,et al.Phim46.1,the main Streptococcus pyogenes element carrying mef(A)and tet(O)genes[J].Antimicrob Agents Chemother,2010,54(1):221-229
[10] Okwumabua O,O’Connor M,Shull E.A polymerase chain reaction(PCR)assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase[J].FEMS Microbiol Lett,2003,218(1):79-84
[11] CLSI.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals:approved standard—third edition//CLSI Document M31-A3.Wayne,PA:Clinical and Laboratory Standards Institute,2008
[12] Sutcliffe J,Grebe T,Tait-Kamradt A,et al.Detection of erythromycin-resistant determinants by PCR[J].Antimicrob Agents Chemother,1996,40(11):2562-2566
[13] Vela A I,Goyache J,Tarradas C,et al.Analysis of genetic diversity of Streptococcus suis clinical isolates from pigs in Spain by pulsed-field gel electrophoresis[J].J Clin Microbiol,2003,41(6):2498-2502
[14] Tenover F C,Arbeit R D,Goering R V,et al.Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:criteria for bacterial strain typing[J].J Clin Microbiol,1995,33(9):2233-2239
[15] 王丽平,陆承平,唐家琪.猪链球菌对大环内酯类抗生素的耐药性及耐药表型[J].南京农业大学学报,2004,27(4):81-87.doi:10.7685/j.issn.1000-2030.2004.04.018
[16] Vela A I,Moreno M A,Cebolla J A,et al.Antimicrobial susceptibility of clinical strains of Streptococcus suis isolated from pigs in Spain[J].Veterinary Microbiology,2005,105(2):143-147
[17] 杨玲丽,陈杖榴,刘健华,等.动物、环境及饲养员多重耐药大肠杆菌的PFGE分型研究[J].中国农业科学,2006,39(12):2597-2602
[18] 聂小想,沈志强,崔言顺,等.脉冲场凝胶电泳技术在猪链球菌分型中的应用[J].动物医学进展,2008,29(1):70-73
[19] 罗隆泽,王鑫,崔志刚,等.四川资阳地区健康猪2型猪链球菌分离与分子生物学特征分析[J].中国人兽共患病学报,2009,25(9):842-845
[20] 杜华茂,丁玉春,王丽丽,等.4株猪链球菌的病原生物学特性分析[J].中国兽医学报,2010,30(1):24-28
[21] Varaldo P E,Montanari M P,Giovanetti E.Genetic elements responsible for erythromycin resistance in Streptococci[J].Antimicrob Agents Chemother,2009,53(2):343-353
[22] Cochetti I,Tili E,Mingoia M,et al.erm(B)-carrying elements in tetracycline-resistant Pneumococci and correspondence between Tn1545 and Tn6003[J].Antimicrob Agents Chemother,2008,52(4):1285-1290
[23] Calatayud L,Ardanuy C,Cercenado E,et al.Serotypes,clones,and mechanisms of resistance of erythromycin-resistant Streptococcus pneumoniae isolates collected in Spain[J].Antimicrob Agents Chemother,2007,51(9):3240-3246
[24] Okitsu N,Kaieda S,Yano H,et al.Characterization of ermB gene transposition by Tn1545 and Tn917 in macrolide-resistant Streptococcus pneumoniae isolates[J].J Clin Microbiol,2005,43(1):168-173
[25] Giovanetti E,Brenciani A,Vecchi M,et al.Prophage association of mef(A)elements encoding efflux-mediated erythromycin resistance in Streptococcus pyogenes[J].J Antimicrob Chemother,2005,55(4):445-451
[26] Banks D J,Porcella S F,Barbian K D,et al.Structure and distribution of an unusual chimeric genetic element encoding macrolide resistance in phylogenetically diverse clones of group A Streptococcus[J].J Infect Dis,2003,188(12):1898-1908

相似文献/References:

[1]王丽平,陆承平.耐药猪链球菌膜蛋白图谱分析及能量抑制剂对其敏感性的影响[J].南京农业大学学报,2007,30(2):94.[doi:10.7685/j.issn.1000-2030.2007.02.019]
 WANG Li-ping,LU Cheng-ping.Membrane protein profile and the effect of enegic inhibitor on the sensibility against resistant Streptococcus strains[J].Journal of Nanjing Agricultural University,2007,30(4):94.[doi:10.7685/j.issn.1000-2030.2007.02.019]
[2]陈国强,姚火春,陆承平.猪链球菌2型溶血毒素的产生条件及其特性分析[J].南京农业大学学报,2000,23(3):71.[doi:10.7685/j.issn.1000-2030.2000.03.019]
 Chen Guoqiang,Yao Huochun,Lu Chengping.Effects of cultural conditions on yield of hemolytic toxin by Streptococcus suis and its characterization[J].Journal of Nanjing Agricultural University,2000,23(4):71.[doi:10.7685/j.issn.1000-2030.2000.03.019]
[3]王丽平,陆承平,唐家琪.猪链球菌对大环内酯类抗生素的耐药性及耐药表型[J].南京农业大学学报,2004,27(4):81.[doi:10.7685/j.issn.1000-2030.2004.04.018]
 WANG Li-ping,LU Cheng-ping,TANG Jia-qi.Trends and phenotypes of resistance to macrolides in Streptococcus suis[J].Journal of Nanjing Agricultural University,2004,27(4):81.[doi:10.7685/j.issn.1000-2030.2004.04.018]
[4]欧瑜,陆承平.检测猪链球菌2型毒力相关蛋白的ELISA法的建立[J].南京农业大学学报,2001,24(2):94.[doi:10.7685/j.issn.1000-2030.2001.02.023]
 Ou Yu,Lu Chengping.Establishing of ELISA for detecting virulence related proteins of Streptococcus suis type 2[J].Journal of Nanjing Agricultural University,2001,24(4):94.[doi:10.7685/j.issn.1000-2030.2001.02.023]
[5]逯朋朋,张越,钟孝俊,等.荚膜聚合酶Wzy对猪链球菌Chz型致病力的影响[J].南京农业大学学报,2018,41(3):489.[doi:10.7685/jnau.201802009]
 LU Pengpeng,ZHANG Yue,ZHONG Xiaojun,et al.Oligosaccharide repeat unit polymerase Wzy affects the pathogenicity in Streptococcus suis serotype Chz[J].Journal of Nanjing Agricultural University,2018,41(4):489.[doi:10.7685/jnau.201802009]

备注/Memo

备注/Memo:
收稿日期:2012-10-11;改回日期:。
基金项目:江苏省自然科学基金项目(BK2012771);国家自然科学基金项目(30972220);中央高校基本科研业务费专项资金项目(6J0396)
作者简介:黄金虎,硕士研究生。
通讯作者:王丽平,博士,教授,主要从事兽医药理及毒理学和细菌耐药性研究,E-mail:wlp71@163.com。
更新日期/Last Update: 1900-01-01