[1]陈慧,张树军,张妤艳,等.40个梨品种S基因型的鉴定及S基因频率分析[J].南京农业大学学报,2013,36(5):21-26.[doi:10.7685/j.issn.1000-2030.2013.05.004]
 CHEN Hui,ZHANG Shujun,ZHANG Yuyan,et al.Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus[J].Journal of Nanjing Agricultural University,2013,36(5):21-26.[doi:10.7685/j.issn.1000-2030.2013.05.004]
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40个梨品种S基因型的鉴定及S基因频率分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年5期
页码:
21-26
栏目:
出版日期:
2013-09-24

文章信息/Info

Title:
Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus
作者:
陈慧 张树军 张妤艳 衡伟 吴俊 张绍铃
南京农业大学梨工程技术研究中心, 江苏 南京 210095
Author(s):
CHEN Hui ZHANG Shujun ZHANG Yuyan HENG Wei WU Jun ZHANG Shaoling
Center of Pear Engineering and Technology Research, Nanjing Agricultural University, Nanjing 210095, China
关键词:
自交不亲和S基因型频率
Keywords:
pearself-incompatibility(SI)S-genotypefrequency
分类号:
S661.2
DOI:
10.7685/j.issn.1000-2030.2013.05.004
摘要:
利用变性聚丙烯酰胺凝胶电泳分离及DNA序列分析,对我国主栽的4个梨系统的40个梨品种的S基因型进行了鉴定。结果表明:‘早生喜水’和‘玉水’,‘湘南’和‘大青梨’,‘水红霄’、‘红霄’和‘云南黄皮水’,‘青皮酥’和‘白皮酥’,‘斯尔克甫梨’和‘沙-01号香梨’都具有相同的S基因型,分别为S3S4S1S3S16S19S34SnS22S28,鉴于梨自花授粉不结实的特性,应避免这些品种间的配置授粉。同时,对这40个梨品种中出现的29个S基因的分布频率进行了分析,结果表明:不同的S基因出现的频率不同,S1S3出现的频率最高为10.0%,其次为S19(8.75%)、S22S34(6.25%),其余S基因出现的频率均低于5%,而相同的S基因在不同梨系统中出现的频率也不同,如S3基因在砂梨系统中出现的频率最高为17.5%。
Abstract:
In this study,forty pear cultivars which belong to four species of Pyrus were used.S-genotypes of them were determined according to the polyacrylamide-gel electrophoresis(PAGE)analysis of PCR products amplified by specific primers and the analysis of DNA sequence.Among them,‘Zaoshengxishui’and‘Yushui’,‘Xiangnan’and‘Daqingli’,‘Shuihongxiao’‘Hongxiao’and‘Yunnanhuangpishui’,‘Qingpisu’and‘Baipisu’,‘Sierkefuli’and‘Sha-01 Xiangli’shared the same S-genotypes,S3S4,S1S3,S16S19,S34Sn and S22S28,respectively.Because of the characteristics of self-incompatibility(SI)in pear,pollination between those cultivars with the same S-genotype should be avoided.Then,the distribution of the frequency of 29 S-RNase genes in the 40 pear cultivars was analyzed.The results showed that the frequency of S-RNase genes were different in Pyrus S-genotypes,among which S1 and S3 performed the highest frequency of 10.0%,followed by S19(8.75%),S22 and S34(6.25%),and all others were less than 5%.The frequency of the same S-RNase genes in different species of Pyrus was different as well.For example,S3 gave the highest frequency in P.pyrifolia Nakai.

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备注/Memo

备注/Memo:
收稿日期:2012-7-20;改回日期:。
基金项目:江苏省科技支撑计划项目(BE2010324);中央高校基本科研业务费专项资金项目(KYZ2000911)
作者简介:陈慧,博士研究生。
通讯作者:张绍铃,教授,主要从事果树分子生物学及发育生物学的研究,E-mail:nnzsl@njau.edu.cn。
更新日期/Last Update: 1900-01-01