[1]杨俊花,罗爱琼,刘丹,等.高剂量碳酸氢钠日粮对蛋壳质量的影响及机制[J].南京农业大学学报,2013,36(6):67-72.[doi:10.7685/j.issn.1000-2030.2013.06.011]
 YANG Junhua,LUO Aiqiong,LIU Dan,et al.The effects and mechanism of dietary supplement with excessive sodium bicarbonate on eggshell quality[J].Journal of Nanjing Agricultural University,2013,36(6):67-72.[doi:10.7685/j.issn.1000-2030.2013.06.011]
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高剂量碳酸氢钠日粮对蛋壳质量的影响及机制()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年6期
页码:
67-72
栏目:
出版日期:
2013-11-20

文章信息/Info

Title:
The effects and mechanism of dietary supplement with excessive sodium bicarbonate on eggshell quality
作者:
杨俊花1 罗爱琼1 刘丹1 缪德年2 赵志辉1
1. 上海市农业科学院农产品质量标准与检测技术研究所, 上海 201403;
2. 上海市农业科学院畜牧兽医研究所, 上海 201106
Author(s):
YANG Junhua1 LUO Aiqiong1 LIU Dan1 MIAO Denian2 ZHAO Zhihui1
1. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;
2. Institute of Animal Husbandry and Veterinary Research, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
关键词:
碳酸氢钠蛋壳质量血钙蛋壳腺钙离子转移蛋鸡
Keywords:
sodium bicarbonateeggshell qualityplasma calciumegg shell glandCa2+ transferlaying hen
分类号:
S852
DOI:
10.7685/j.issn.1000-2030.2013.06.011
摘要:
为探讨日粮添加碳酸氢钠(NaHCO3)对蛋壳质量、蛋壳钙、血钙浓度、钙调激素水平以及蛋壳腺中Ca2+转移相关蛋白基因表达的影响,选取900只21周龄海兰褐蛋鸡随机分为对照组和1、5、25和50 g·kg-1 NaHCO3添加组,每组6个重复,每个重复30只,持续10周。结果表明:与对照组相比,日粮添加1和5 g·kg-1 NaHCO3对蛋壳质量不产生显著影响(P>0.05),25和50 g·kg-1组蛋壳钙含量显著降低(P<0.01),蛋壳质量、厚度降低(P>0.05),50 g·kg-1组蛋壳强度和血清Ca2+浓度亦显著降低(P<0.05)。此外,随着NaHCO3添加量的增加,25和50 g·kg-1组血液维生素D水平显著或极显著增加(P<0.05或P<0.01),蛋壳腺中瞬时性受体电位通道香草精受体6(TRPV6)、钙结合蛋白(CaBP-D28k)、钠-钙交换蛋白(PMCA 1b)和质膜钙离子ATP酶通道(NCX1)mRNA表达量显著提高(P<0.05),50 g·kg-1组碳酸酐酶(CA)mRNA水平显著降低(P<0.05)。结论:高剂量的NaHCO3(≥25 g·kg-1)使蛋壳质量下降,CA mRNA表达量降低,致使蛋壳腺Ca2+转移受阻,蛋壳钙含量减少、质量下降。其次,低血钙和高维生素D水平使TRPV6、CaBP-D28k、PMCA 1bNCX1等基因表达增加,表明钙离子跨细胞转运途径在蛋壳腺中起补充转移作用。
Abstract:
The purpose of this study was to analyze the effect of dietary NaHCO3 on eggshell quality,the concentration of Ca2+ in eggshell and plasma,and levels of plasma calcium regulating hormones,as well as mRNA expression of genes related to Ca2+ transport in eggshell gland(ESG).Nine hundred 21-weeks-old Hy-line brown laying hens were randomly assigned to be fed with 0,1,5,25 and 50 g·kg-1 NaHCO3 for 10 weeks,and each group was composed of 6 replicates and 30 birds per replicate.The results showed as follows:compared with control,the eggshell quality was not influenced by dietary NaHCO3 level at 1 g·kg-1 or 5 g·kg-1(P>0.05).However,the content of Ca2+ in eggshell(P<0.01),eggshell thickness and weight(P>0.05)were reduced in 25 g·kg-1 and 50 g·kg-1 groups,respectively.In addition,eggshell strength and plasma Ca2+ content were also significantly reduced by the supplementation of 50 g·kg-1 NaHCO3(P<0.05).Then,the concentration of vitamin D level in plasma was dramatically increased by dietary NaHCO3 level at 25 and 50 g·kg-1(P<0.05 or P<0.01).Moreover,the mRNA expression of transient receptor potential vanilloid receptor 6(TRPV6),calbindin-D28k(CaBP-D28k),plasma membrane Ca2+-ATPase(PMCA 1b)and Na+/Ca2+ exchangers(NCX1)in ESG tissue were increased in 25 and 50 g·kg-1 NaHCO3 groups(P<0.05),but the level of carbonic anhydrase(CA)mRNA was remarkably reduced in 50 g·kg-1 group(P<0.05).In conclusion:the mechanism was that lower plasma Ca2+ content and CA mRNA level were induced by excessive NaHCO3(≥25 g·kg-1),then which led to block to Ca2+ transfer in ESG,finally resulted in the reduction of eggshell quality.Secondly,the increase in mRNA expression of TRPV6,CaBP-D28k,PMCA 1b,and NCX1 suggests that transcellular Ca2+ transport pathway appears to compensate for low Ca2+ intake in ESG.

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备注/Memo

备注/Memo:
收稿日期:2013-1-23;改回日期:。
基金项目:国家公益性行业(农业)科研专项(201203015);上海市科委公共服务平台建设专项(11DZ2292800);上海市科技兴农重点攻关项目
作者简介:杨俊花,博士,E-mail:yangjunhua303@126.com。
通讯作者:赵志辉,研究员,研究方向为饲料及饲料添加剂安全评价,E-mail:zhao9912@hotmail.com。
更新日期/Last Update: 1900-01-01