[1]张丽,韩兆玉.甜菜碱对夏季热应激奶牛产奶性能和HSP70及其mRNA表达的影响[J].南京农业大学学报,2013,36(6):89-94.[doi:10.7685/j.issn.1000-2030.2013.06.014]
 ZHANG Li,HAN Zhaoyu.Effects of supplementing betaine on milk performance, HSP70 and HSP70 mRNA level of cows under heat stress[J].Journal of Nanjing Agricultural University,2013,36(6):89-94.[doi:10.7685/j.issn.1000-2030.2013.06.014]
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甜菜碱对夏季热应激奶牛产奶性能和HSP70及其mRNA表达的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年6期
页码:
89-94
栏目:
出版日期:
2013-11-20

文章信息/Info

Title:
Effects of supplementing betaine on milk performance, HSP70 and HSP70 mRNA level of cows under heat stress
作者:
张丽 韩兆玉
南京农业大学动物科技学院, 江苏 南京 210095
Author(s):
ZHANG Li HAN Zhaoyu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
甜菜碱热应激HSP70 mRNA奶牛
Keywords:
betaineheat stressHSP70 mRNAdairy cow
分类号:
S852.21
DOI:
10.7685/j.issn.1000-2030.2013.06.014
摘要:
为研究日粮中添加不同剂量甜菜碱对夏季热应激奶牛生产性能和HSP70及其mRNA表达的影响,根据年龄、胎次、泌乳期、产奶量相近的原则选取32头荷斯坦奶牛,随机均分为4组,对照组(C)不添加甜菜碱,试验Ⅰ、Ⅱ、Ⅲ组分别在日粮中添加10、15和20 g·d-1甜菜碱。结果表明:试验期牛舍平均温湿指数(THI)为78.68;试验Ⅱ和Ⅲ组奶牛产奶量比对照组和试验Ⅰ组显著提高(P<0.05);试验Ⅱ组乳糖含量显著高于对照组和试验Ⅲ组(P<0.05),试验Ⅰ和Ⅲ组显著高于对照组(P<0.05);试验Ⅱ组乳蛋白率显著高于对照组(P<0.05);试验后期试验Ⅱ组奶牛外周血HSP70含量与对照组相比显著降低(P<0.05)。结论:添加甜菜碱可提高奶牛生产性能,且以添加15 g·d-1的效果最优;添加15 g·d-1甜菜碱后降低了奶牛外周血HSP70含量,因而缓解了奶牛热应激。
Abstract:
This study was conducted to study the effect of supplementing betaine on milk performance,HSP70 and HSP70 mRNA level of cows under heat stress.Thirty-two lactating Holstein cows were randomly divided into 4 treatments(8 cows per treatment)according to the age,stage of lactation and milk yield,one of four supplements was blended into the total mixed ration(TMR)to produce 4 dietary treatments:control group(C),treatmentⅠgroup(Ⅰ,10 g·d-1 of betaine),treatmentⅡgroup(Ⅱ,15 g·d-1 of betaine),and treatment Ⅲ group(Ⅲ,20 g·d-1 of betaine).The results showed that:the average temperature-humidity index(THI)was 78.68 units.Milk yield was higher inⅡand Ⅲ group cows(P<0.05)compared with C andⅠgroup cows.Milk lactose percentage was higher inⅡgroup cows(P<0.05)compared with C and Ⅲ group cows,and milk lactose percentage of bothⅠand Ⅲ group cows increased(P<0.05)compared with C group cows.Milk protein percentage was higher(P<0.05)inⅡgroup cows compared with C group cows.HSP70 protein in group Ⅱ was lower compared with C and Ⅲ group cows(P<0.05)in the end.These results indicated that the supplementary of betaine could improve the milk performance of cows under heat stress,and Ⅱ group showed the most effective influence and was better to reduce heat stress.

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备注/Memo

备注/Memo:
收稿日期:2012-12-28;改回日期:。
基金项目:国家"十二五"科技支撑计划项目(2012BAD12B00)
作者简介:张丽,硕士研究生。
通讯作者:韩兆玉,博士,副教授,主要从事反刍动物生长调控研究,E-mail:zyhan6708@njau.edu.cn。
更新日期/Last Update: 1900-01-01