XU Tongjian,ZHONG Gang,WANG Xichun,et al.Effects of lipid coated γ-aminobutyric acid on HPA axis and some immune indicators of cows in heat stress[J].Journal of Nanjing Agricultural University,2014,37(1):127-132.[doi:10.7685/j.issn.1000-2030.2014.01.021]





Effects of lipid coated γ-aminobutyric acid on HPA axis and some immune indicators of cows in heat stress
徐同锏12 钟刚13 王希春1 吴金节1 李锦春1
1. 安徽农业大学动物科技学院, 安徽 合肥 230036;
2. 北京伟嘉集团公司, 北京 100085;
3. 天津宝迪农业科技股份有限公司, 天津 301800
XU Tongjian12 ZHONG Gang13 WANG Xichun1 WU Jinjie1 LI Jinchun1
1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Bejing Vica Group Comanpy, Bejing 100085, China;
3. Tianjin Baodi Agriculture Technologies Co.Ltd., Tianjin 301800, China
lipid coated γ-aminobutyric acidheat stresscowHPA axisimmune
为了解脂肪包被γ-氨基丁酸(GABA)对奶牛持续热应激的缓解效果,选用20头荷斯坦奶牛,随机均分为4组,每组5头,Ⅰ(对照组)、Ⅱ、Ⅲ、Ⅳ组分别饲喂脂肪包被GABA(40%纯度)0、5、10和15 g·d-1。结果表明:饲喂15 d,各试验组血清GABA水平均高于Ⅰ组,且Ⅳ与Ⅰ组间差异显著(P<0.05);Ⅲ、Ⅳ组的促肾上腺激素释放激素(CRH)显著低于Ⅰ、Ⅱ组;Ⅳ组白介素(IL-2)显著高于其他3组;Ⅳ组IgG极显著高于Ⅰ、Ⅱ组(P<0.01)。饲喂30 d,Ⅲ、Ⅳ组血清GABA极显著高于Ⅰ、Ⅱ组;Ⅳ组促肾上腺激素(ACTH)极显著低于Ⅰ组;Ⅳ组皮质醇(COR)显著低于Ⅰ和Ⅱ组;Ⅳ组IL-2显著高于Ⅰ组;Ⅲ和Ⅳ组IgM显著高于Ⅰ组。添加脂肪包被GABA降低了奶牛直肠温度和呼吸频率,且在10和30 d,Ⅲ、Ⅳ组直肠温度极显著低于Ⅰ组,Ⅳ组呼吸频率显著低于Ⅰ组。结论:添加脂肪包被GABA,抑制了HPA轴激素的分泌,增强了机体的免疫力,其中以添加GABA 15 g·d-1效果最好。
To investigate the alleviate effect of lipid coated γ-aminobutyric acid(GABA)on the continuous heat stress of cow,20 Holstein cows were selected and randomly divided into four groups with five replicates in each group,control groupⅠ,experimental group Ⅱ,Ⅲ and Ⅳ.The four groups were fed lipid coated GABA(40% purity)at the amount of 0,5,10 and 15 g·d-1,respectively.The results showed that the serum GABA concentration of each experimental group was higher than that of control group after 15 days and group Ⅳ was significantly different between groupⅠ(P<0.05);The concentrations of adrenocorticotropic hormone releasing hormone(CRH)of group Ⅲ and Ⅳ were significantly less than those of groupⅠand Ⅱ(P<0.05);The serum IL-2 concentration of group Ⅳ was significantly higher than those of the other three groups(P<0.05);The serum IgG concentration of group Ⅳ was extremely significantly higher than those of groupⅠand Ⅱ(P<0.01).The serum GABA concentration of group Ⅲ and Ⅳ were extremely significantly higher than those of groupⅠand Ⅱ after 30 days;The serum adrenocorticotropic hormone(ACTH)concentration of group Ⅳ was markedly significantly lower than those of groupⅠ;The serum corticosterone(COR)concentration of group Ⅳ was significantly lower than those of groupⅠand Ⅱ;The serum IL-2 concentration of group Ⅳ was significantly higher than that of groupⅠ(P<0.05);The serum IgM concentration of group Ⅲ and Ⅳ were significantly higher than that of groupⅠ(P<0.05).The rectal temperature and respiratory rate of cows were reduced with the addition of the coated GABA,and in the 10 and 30 days,the rectal temperature of group Ⅲ and Ⅳ was markedly significantly lower than that of groupⅠ(P<0.01),the respiratory rate of group Ⅳ was significantly lower than that of groupⅠ.Conclusion:The hypothalamus-pituitary gland-adrenal gland(HPA)axis hormone production and the body immunity were enhanced by feeding coated.Feeding 15 g·d-1 of GABA has the best effect in this experiment.


[1] 杜红芳, 窦爱丽, 杨威, 等.热应激对奶牛的生理变化及缓解措施[J].中国畜牧杂志, 2007, 43(12):59-62
[2] West J W, Mullinix B G, Bernard J K.Effects of hot, humid weather on milk temperature, dry matter intake and milk yield of lactating dairy cows[J].J Dairy Sci, 2003, 86:23-24
[3] 郭亮, 杜鹃, 狄和双, 等.单一或连续高温处理对奶牛乳腺细胞的生长及凋亡的影响[J].南京农业大学学报, 2007, 30(3):100-104.doi:10.7685/j.issn.1000-2030.2007.03.019
[4] 邹胜龙, 冯定远.动物热应激机理及其研究进展[J].广东饲料, 2000, 9(4):20-22
[5] 李建国, 桑润滋, 张正珊, 等.热应激对奶牛生理常值、血液生化指标、繁殖及泌乳性能的影响[J].河北农业大学学报, 1998, 21(4):69-74
[6] Xu X G, Yang Z F, Huang S H, et al.Promotive effects of GABA on acid secretion from isolated mouse stomach in vitro[J].Acta Zoologica Sinica, 2001, 47(2):170-175
[7] Stratfold T R, Kelley A E.GABA in the nucleus accumbens shell participates in the central regulation of feeding behavior[J].J Neurosci, 1997, 17(11):4434-4440
[8] Jonaidi H, Babapour V, Denbow D M.GABAergic control of food intake in the meat-type chickens[J].Physiol Behav, 2002, 76(4/5):465-468
[9] Tajalli S, Jonaidi H, Abbasnejad M, et al.Interaction between nociceptin/orphanin FQ(N/OFQ)and GABA in response to feeding[J].Physiol Behav, 2006, 89(3):410-413
[10] Fan Z Y, Deng J P, Liu G H, et al.Study on the effect of γ-aminobutyric acid on the reproduction performance and internal hormone levels in growing pigs[J].动物营养学报, 2007, 19(4):350-356
[11] 胡家澄, 邹晓庭, 董金格.γ-氨基丁酸对畜禽免疫性能的影响及其作用机理研究[J].中国饲料, 2008(18):21-23
[12] 何熙璞, 张敏, 李俊芳.γ-氨基丁酸的生理学功能及研究现状[J].广西大学学报:自然科学版, 2007, 32(增刊):464-466
[13] 曹德瑞, 邹晓庭.γ-氨基丁酸对生长肥育猪生长性能和胴体品质的影响[J].饲料工业, 2007, 28(23):31-34
[14] 李慧, 赵文静, 邹晓庭, 等.γ-氨基丁酸对夏季高温期蛋鸡产蛋性能、蛋品质及血液生化指标的影响[J].饲料工业, 2010, 31(13):17-20
[15] 徐秋良, 张庆莉, 唐国盘.γ-氨基丁酸对高温环境下生长肥育猪生产性能及激素水平的影响[J].家畜生态学报, 2009, 30(6):28-32
[16] 李慧, 唐江飞, 孙涛, 等.γ-氨基丁酸对夏季高温期蛋鸡产蛋性能、蛋品质的影响及其机理研究[J].动物营养学报, 2010, 22(6):1745-1751
[17] 夏磊, 占秀安.γ-氨基丁酸对热应激肉种鸡产蛋性能和繁殖性能的影响[J].动物营养学报, 2012, 24(1):137-144
[18] Mader T L, Davis M S, Brown-Brandl T.Environmental factors influencing heat stress in feedlot cattle[J].J Anim Sci, 2006, 84:712-719
[19] Imaki T, Shibasaki T, Demura H.Regulation of gene expression in the central nervous system by stress:molecular pathways of stress responses[J].Endocr Journal, 1995, 42(2):121-130
[20] 李林, 徐辰义.动物热应激反应机制研究进展[J].动物医学进展, 2011, 32(4):95-98
[21] Herman J P, Mueller N K, Figueiredo H.Role of GABA and glutamate circuitry in hypothalamo-pituitary-adrenocortical stress integration[J].Ann N Y Acad Sci, 2004, 10(18):35-45
[22] Akinci M K, Schofield P R.Widespread expression of GABA(A)receptor subunits in peripheral tissues[J].Neuro Sci Res, 1999, 35(2):145-153
[23] Boudaba C, Szabo K, Tasker J G.Physiological mapping of local inhibitory inputs to the hypothalamic paraventricular nucleus[J].Neurosci, 1996, 16:7151-7160
[24] Frosini M.Changes in CSF composition during heat stress and fever in conscious rabbits[J].Progress in Brain Research, 2007, 162:449-456
[25] Marquesde S L, Franci C R.GABAergic mediation of stress-induced secretion of corticosterone and oxytocin, but not prolactin, by the hypotha-lamic paraventricular nucleus[J].Life Sci, 2008, 83(19/20):686-692
[26] Thaxton P, Siegel H S.Immunodepression in young chickens by high environmental temperature[J].Poultry Science, 1970, 49:202-205
[27] Mashaly M M, Hendricks G L Ⅲ, Kalama M A, et al.Effect of heat stress on production parameters and immune responses of commercial laying hens[J].Poultry Science, 2004, 83:889-894
[28] Nathan D B, Heller E D, Perek M.The effect of short heat stress upon leucocyte count, plasma corticosterone level, plasma and leucocyte ascorbic acid content[J].British Poultry Science, 1976, 17:481-485
[29] 蔡亚非, 李莲, 刘庆华, 等.热应激奶牛外周淋巴细胞的凋亡和bax-α基因表达[J].南京农业大学学报, 2005, 28(1):66-70.doi:10.7685/j.issn.1000-2030.2005.01.015
[30] Zulkifli I, Chenorma M T, Israf D A, et al.The effect of early age feed restriction on subsequent response to high environmental temperatures in female broiler chickens[J].Poultry Science, 2000, 79:1401-1407
[31] Abdou A M, Higashiguchi S, Horie K, et al.Relaxation and immunity enhancement effects of gamma-aminobutyric acid(GABA)administration in humans[J].Biofactors, 2006, 26:201-208
[32] Zhang M, Zou X T.Effect of dietary gamma-aminobutyric acid on laying performance, egg quality, immune activity and endocrine hormone in heat-stressed Roman hens[J].Animal Science Journal, 2012, 83:141-147
[33] 杨汉春.动物免疫学[M].2版, 北京:中国农业大学出版社, 2003:176-177
[34] 路翠燕, 潘芳.应激反应中HPA轴的中枢调控和免疫调节[J].中国行为医学科学, 2003, 12(3):353-355
[35] 胡家澄, 邹晓庭, 董金格.γ-氨基丁酸对肥育猪免疫机能的影响及其作用机理[J].饲料工业, 2008, 29(14):3-5


[1]廖旭东,崔群维,周璇,等.公牛HSP 基因多态性与其精液品质及后裔性能的相关分析[J].南京农业大学学报,2011,34(4):105.[doi:10.7685/j.issn.1000-2030.2011.04.019]
 LIAO Xu-dong,CUI Qun-wei,ZHOU Xuan,et al.The correlation analysis between bulls HSP gene polymorphism and semen quality and progeny performance[J].Journal of Nanjing Agricultural University,2011,34(1):105.[doi:10.7685/j.issn.1000-2030.2011.04.019]
 ZHANG Li,HAN Zhaoyu.Effects of supplementing betaine on milk performance, HSP70 and HSP70 mRNA level of cows under heat stress[J].Journal of Nanjing Agricultural University,2013,36(1):89.[doi:10.7685/j.issn.1000-2030.2013.06.014]
 ZHENG Yue,CHEN Kunlin,LI Huixia,et al.Profiling of differential expression of microRNAs in serum of heat-stresseded cows and bioinformatic analyses of miR-181a target genes[J].Journal of Nanjing Agricultural University,2014,37(1):130.[doi:10.7685/j.issn.1000-2030.2014.06.019]
 BAI Wujiao,JIN Pengjin,KUANG Meiqian,et al.Effects of heat stress on the pathway associated with luteal progesterone metabolism[J].Journal of Nanjing Agricultural University,2017,40(1):145.[doi:10.7685/jnau.201601018]
 WANG Huanhuan,SHEN Zhengjie,XIAO Hang,et al.Effects of heat stress on Keap1-Nrf2-ARE signal pathway of liver in dairy cows[J].Journal of Nanjing Agricultural University,2017,40(1):151.[doi:10.7685/jnau.201602023]
 SONG Zhihua,CHENG Kang,ZHANG Jingfei,et al.Effects of dietary enzymatically treated Artemisia annua supplementation on digestion function of the heat-stressed broilers[J].Journal of Nanjing Agricultural University,2018,41(1):126.[doi:10.7685/jnau.201612017]


更新日期/Last Update: 1900-01-01