WANG Chao,ZHANG Quanjun,HUANG Xiaosan,et al.Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear[J].Journal of Nanjing Agricultural University,2014,37(2):26-32.[doi:10.7685/j.issn.1000-2030.2014.02.005]





Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear
王超 张全军 黄小三 赵碧英 杨雅楠 吴俊
南京农业大学园艺学院, 江苏 南京 210095
WANG Chao ZHANG Quanjun HUANG Xiaosan ZHAO Biying YANG Yanan WU Jun
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
pearPbLFY genePbTFL1 genePbFT geneexpression patterns
The genes fragment for LFY(PbLFY),and whole length of TFL1(PbTFL1,KF240776)and FT(PbFT,KF240775)were isolated from‘Jinzhui’pear(Pyrus bretschneideri).Real-time quantitative PCR(qPCR)was used to examine the expression profiles of the three genes in different tissues and different periods of floral bud development.The transcript levels of PbLFY were the highest levels in floral bud,and were not detected in young leaf and stamen,which showed higher expression at the beginning of bud differentation,and kept the higher level until reaching the highest level when floral organ primordia appeared.Expression of PbTFL1 was the highest in floral bud,but was not observed in young leaf,mature flower and floral organs.PbTFL1 transcript level reached the highest level at the beginning of floral bud development,which exhibited a decrease trend gradually,then decreased extremely at the beginning of floral bud differentiation.PbFT transcript level was detected in all tissues,which showed the highest level in mature leaf,followed by floral bud.The transcript levels of PbFT was lower at the early stage of floral bud development,and then increased steadily until reaching the highest level at the beginning of floral differentiation.The difference in expression profiles of those three genes showed that they played a different role in the process of flowering development,and this research demonstrated that both PbLFY and PbFT promoted the flowering development of‘Jinzhui’pear,while PbTFL1 played negative function.


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更新日期/Last Update: 1900-01-01