[1]王超,张全军,黄小三,等.‘金坠’梨成花相关基因PbLFY、PbTFL1和PbFT的克隆及时空表达模式分析[J].南京农业大学学报,2014,37(2):26-32.[doi:10.7685/j.issn.1000-2030.2014.02.005]
 WANG Chao,ZHANG Quanjun,HUANG Xiaosan,et al.Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear[J].Journal of Nanjing Agricultural University,2014,37(2):26-32.[doi:10.7685/j.issn.1000-2030.2014.02.005]
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‘金坠’梨成花相关基因PbLFY、PbTFL1PbFT的克隆及时空表达模式分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
37卷
期数:
2014年2期
页码:
26-32
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear
作者:
王超 张全军 黄小三 赵碧英 杨雅楠 吴俊
南京农业大学园艺学院, 江苏 南京 210095
Author(s):
WANG Chao ZHANG Quanjun HUANG Xiaosan ZHAO Biying YANG Yanan WU Jun
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
PbLFY基因PbTFL1基因PbFT基因时空表达
Keywords:
pearPbLFY genePbTFL1 genePbFT geneexpression patterns
分类号:
S661.2
DOI:
10.7685/j.issn.1000-2030.2014.02.005
摘要:
以‘金坠’梨为试材,分别克隆了LFY同源基因PbLFY片段、TFL1同源基因PbTFL1(KF240776)和FT同源基因PbFT(KF240775)的全长序列,同时对不同组织器官和花芽发育的不同时期进行实时定量PCR分析。结果表明:PbLFY在花芽中表达量最高,在嫩叶和雄蕊中不表达,在花芽开始分化期表达量升高,并维持着较高的表达水平,在花器官原基出现时达到最大值;PbTFL1在花芽中表达水平最高,在嫩叶、成熟花和花器官中不表达,在花芽发育的早期表达量最高,并逐渐下降,花芽开始分化时表达量下降迅速;PbFT在各组织器官中都有表达,在成熟叶片中表达量最高,花芽中表达量次之,花芽发育早期表达量较低,并逐步增加,花芽开始分化时表达量最高。这3个基因表达模式的差异,表明它们在梨成花的过程中起到不同的作用,PbLFYPbFT表现为促进成花转变,PbTFL1主要表现抑制成花作用。
Abstract:
The genes fragment for LFY(PbLFY),and whole length of TFL1(PbTFL1,KF240776)and FT(PbFT,KF240775)were isolated from‘Jinzhui’pear(Pyrus bretschneideri).Real-time quantitative PCR(qPCR)was used to examine the expression profiles of the three genes in different tissues and different periods of floral bud development.The transcript levels of PbLFY were the highest levels in floral bud,and were not detected in young leaf and stamen,which showed higher expression at the beginning of bud differentation,and kept the higher level until reaching the highest level when floral organ primordia appeared.Expression of PbTFL1 was the highest in floral bud,but was not observed in young leaf,mature flower and floral organs.PbTFL1 transcript level reached the highest level at the beginning of floral bud development,which exhibited a decrease trend gradually,then decreased extremely at the beginning of floral bud differentiation.PbFT transcript level was detected in all tissues,which showed the highest level in mature leaf,followed by floral bud.The transcript levels of PbFT was lower at the early stage of floral bud development,and then increased steadily until reaching the highest level at the beginning of floral differentiation.The difference in expression profiles of those three genes showed that they played a different role in the process of flowering development,and this research demonstrated that both PbLFY and PbFT promoted the flowering development of‘Jinzhui’pear,while PbTFL1 played negative function.

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备注/Memo

备注/Memo:
收稿日期:2013-4-28;改回日期:。
基金项目:国家梨产业技术体系项目(CARS-29);江苏省科技支撑计划项目(BE2011320)
作者简介:王超,硕士研究生。
通讯作者:吴俊,教授,博导,从事果树分子育种研究,E-mail:wujun@njau.edu.cn。
更新日期/Last Update: 1900-01-01