[1]李红娟,刘廷利,刘莉,等.转棉花Gbve1基因拟南芥抗黄萎菌的细胞学机制分析[J].南京农业大学学报,2014,37(2):53-58.[doi:10.7685/j.issn.1000-2030.2014.02.009]
 LI Hongjuan,LIU Tingli,LIU Li,et al.Preliminary study on the mechanism of transgenic Gbve1 Arabidopsis resistance to Verticillium dahliae[J].Journal of Nanjing Agricultural University,2014,37(2):53-58.[doi:10.7685/j.issn.1000-2030.2014.02.009]
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转棉花Gbve1基因拟南芥抗黄萎菌的细胞学机制分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
37卷
期数:
2014年2期
页码:
53-58
栏目:
出版日期:
2014-03-20

文章信息/Info

Title:
Preliminary study on the mechanism of transgenic Gbve1 Arabidopsis resistance to Verticillium dahliae
作者:
李红娟1 刘廷利1 刘莉1 张保龙2 窦道龙1
1. 南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095;
2. 江苏省农业科学院农业生物技术研究所, 江苏 南京 210014
Author(s):
LI Hongjuan1 LIU Tingli1 LIU Li1 ZHANG Baolong2 DOU Daolong1
1. College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Agricultural Biotechnology, Jiangsu Province Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
大丽轮枝菌Gbve1基因拟南芥亚细胞定位抗病机制
Keywords:
Verticillium dahliaeGbve1 geneArabidopsissubcellular localizationresistance mechanism
分类号:
S435.621.2+4
DOI:
10.7685/j.issn.1000-2030.2014.02.009
摘要:
Gbve1是海岛棉中的1个抗棉花黄萎菌基因,前期研究表明它能介导棉花与拟南芥对落叶型与非落叶型黄萎菌的抗性。为了揭示该基因的作用机制,对其细胞定位进行了研究。结果表明:该基因定位在植物细胞膜上;并检测了胼胝质沉积和活性氧积累情况,发现转基因拟南芥在黄萎菌侵染后胼胝质沉积,过氧化氢含量明显高于对照植株。推测:Gbve1是1个膜定位蛋白基因,它能响应黄萎菌的侵染,诱导拟南芥产生抗病性。
Abstract:
Gbve1,which was isolated from the island cotton cultivar,encodes a cell membrane receptor-like protein against Verticillium dahliae.To further reveal the mechanism of action of Gbve1 gene,Agrobacterium-mediated transient expression technology was used to study its cellular localization in tobacco.The results showed that the protein was located in the plant cell membrane.In order to study the mechanism of resistance,we examined callose deposition in Gbve1-transgenic Arabidopsis post V.dahliae infection.The results showed the callose deposition was significantly stronger than that of the control plants;reactive oxygen test results showed that hydrogen peroxide in transgenic Arabidopsis after inoculation was also significantly higher than that in the control plants.Collectively,we gained a better understanding of the resistance mechanism of Gbve1 gene,and provided a scientific basis for its effective use in agriculture.

参考文献/References:

[1] 张保龙, 承泓良, 杨郁文.棉花抗黄萎病研究进展[M].北京:中国农业科学技术出版社, 2012:5-11, 27-28
[2] Klosterman S J, Atallah Z K, Vallad G E, et al.Diversity, pathogenicity, and management of Verticillium species[J].Annu Rev Phytopathol, 2009, 47:39-62
[3] Kawchuk L M, Lynch D R, Hachey J, et al.Identification of a codominant amplified polymorphic DNA marker linked to the Verticillium wilt resistance gene in tomato[J].Theor Appl Genet, 1994, 89:661-664
[4] Kawchuk L M, Hachey J, Lynch D R, et al.Tomato Ve disease resistance genes encode cell surface-like receptors[J].Proc Natl Acad Sci USA, 2001, 98:6511-6515
[5] Rogers S, Wells R, Rechsteiner M.Amino-acid-sequences common to rapidly degraded proteins:the pest hypothesis[J].Science, 1986, 234:364-368
[6] Remy I, Wilson I A, Michnick S W.Erythropoietin receptor activation by a ligand-induced conformation change[J].Science, 1999, 283:990-993
[7] Fradin E F, Zhang Z, Ayala J C J, et al.Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1[J].Plant Physiol, 2009, 150:320-332
[8] Fradin E F, Abd-El-Haliem A, Masini L, et al.Interfamily transfer of tomato Ve1 mediates Verticillium resistance in Arabidopsis[J].Plant Physiol, 2011, 156:2255-2265
[9] Gao X Q, Wheeler T, Li Z H, et al.Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt[J].Plant Journal, 2011, 66:293-305
[10] 陈捷胤.海岛棉LRR-TM类抗病基因GbaVdlGbaVd2的克隆与功能研究[D].北京:中国农业科学院作物科学研究所, 2010
[11] Zhang B L, Yang Y W, Chen T Z, et al.Island cotton Gbve1 gene encoding a receptor-like protein confers resistance to both defoliating and non-defoliating isolates of Verticillium dahliae[J].PLoS One, 2012, 7:e51091
[12] 齐俊生, 李怀方.一种检测棉花黄萎菌毒素致萎性的新方法——叶片针刺涂抹法[J].棉花学报, 2006, 18(4):228-232
[13] 郭志英, 薛泉宏, 张晓鹿, 等.生防菌苗床接种对辣椒根域微生态及产量的影响[J].西北农林科技大学学报:自然科学版, 2008, 36(4):159-165
[14] 刘廷利, 茹艳艳, 刘莉, 等.大豆疫霉效应因子PsCRN115诱导烟草抗性的机制分析[J].南京农业大学学报, 2012, 35(4):65-68.doi:10.7685/j.issn.1000-2030.2012.04.012
[15] 缪卫国, 宋从凤, 王金生, 等.转hpalxoo基因棉花活性氧产生及防卫相关基因的表达[J].南京农业大学学报, 2011, 34(2):61-66.doi:10.7685/j.issn.1000-2030.2011.02.011
[16] Jiang M Y, Zhang J H.Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings[J].Plant Cell Physiol, 2001, 42:1265-1273
[17] Hauck P, Thilmony R, He S Y.A Pseudomonas syringae type Ⅲ effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants[J].Proc Natl Acad Sci USA, 2003, 100:8577-8582
[18] 张小莉, 王鹏程, 宋纯鹏.植物细胞过氧化氢的测定方法[J].植物学报, 2009, 44(1):103-106
[19] 张兴华, 李捷.棉黄萎病发生和研究进展[J].江西农业学报, 2006, 18(1):99-104
[20] Bhat R G, Subbarao K V.Host range specificity in Verticillium dahliae[J].Phytopathology, 1999, 89:1218-1225
[21] Gayoso C, Pomar F, Novo-Uzal E, et al.The Ve-mediated resistance response of the tomato to Verticillium dahliae involves H2O2, peroxidase and lignins and drives PAL gene expression[J].BMC Plant Biol, 2010, 10:232

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备注/Memo

备注/Memo:
收稿日期:2013-6-10;改回日期:。
基金项目:江苏省自然科学基金项目(BK2012027);国家自然科学基金项目(31171831)
作者简介:李红娟,硕士研究生。
通讯作者:窦道龙,教授,主要从事植物与疫霉互作机制研究,E-mail:ddou@njau.edu.cn。
更新日期/Last Update: 1900-01-01