[1]苏昱,蔡天明.1株溴苯腈降解菌的分离鉴定及降解特性研究[J].南京农业大学学报,2014,37(4):97-102.[doi:10.7685/j.issn.1000-2030.2014.04.014]
 SU Yu,CAI Tianming.Isolation and identification of a bromoxynil degradation strain and its degradation characteristics[J].Journal of Nanjing Agricultural University,2014,37(4):97-102.[doi:10.7685/j.issn.1000-2030.2014.04.014]
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1株溴苯腈降解菌的分离鉴定及降解特性研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
37卷
期数:
2014年4期
页码:
97-102
栏目:
出版日期:
2014-06-25

文章信息/Info

Title:
Isolation and identification of a bromoxynil degradation strain and its degradation characteristics
作者:
苏昱 蔡天明
南京农业大学资源与环境科学学院, 江苏 南京 210095
Author(s):
SU Yu CAI Tianming
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
溴苯腈降解菌分离鉴定生物降解
Keywords:
bromoxynildegrading bacteriaisolation and identificationbiodegradation
分类号:
X172
DOI:
10.7685/j.issn.1000-2030.2014.04.014
摘要:
从农药厂污水处理池的活性污泥中分离得到1株能够以溴苯腈为唯一碳源的菌株,命名为XBJ。对菌株XBJ进行生理生化鉴定及16S rRNA基因序列分析,研究其在不同的初始pH、温度、NaCl浓度、通气量情况下的生长情况,以及在不同的初始pH、温度、初始溴苯腈浓度、接种量情况下对溴苯腈的降解情况。结果表明:经生理生化分析以及16S rRNA基因序列比对分析,将菌株XBJ初步鉴定为Ochrobactrum sp.。菌株XBJ在30 ℃、pH 7.0的LB培养基中生长最佳。菌株XBJ降解溴苯腈的最适条件为:30 ℃、pH 7.0。当溴苯腈质量浓度为100 mg·L-1,接种量为5%时,72 h降解率达到91.87%以上。提高接种量有利于加快溴苯腈的降解,当接种量提高到10%时,72 h溴苯腈残留质量浓度为1.53 mg·L-1
Abstract:
In this paper,a highly efficient bromoxynil degrading strain was isolated from the pesticide wastewater treatment pool by enrichment and separation technology,and the strain was named as XBJ.Based on its physiological and biochemical properties and 16S rRNA gene sequence analysis of the strain,the growth of the strain under different environmental conditions was studied,as well as the degradation of bromoxynil under different environmental conditions.The results showed that a bromoxynil-degrading strain XBJ was isolated from pesticide wastewater treatment pool.This strain was primarily identified as Ochrobactrum sp.based on its physiological and biochemical characteristics and 16S rRNA gene sequence.The optimal pH and temperature for the degradation of bromoxynil by strain XBJ were 30 ℃ and pH 7.0.Strain XBJ degradated bromoxynil in 72 h by 91.87% when the initial concentration of bromoxynil was 100 mg·L-1,and with 5% inoculating quantity.Improving inoculation rate speeded up the degradation of bromoxynil.With 10% inoculating quantity,the residual concentration of bromoxynil was only 1.53 mg·L-1 in 72 h.

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备注/Memo

备注/Memo:
收稿日期:2013-10-28。
基金项目:江苏省太湖水污染治理专项资金技术师范类科研课题(TH2012203,TH2013210)
作者简介:苏昱,硕士研究生。
通讯作者:蔡天明,教授,主要从事污水处理研究,E-mail:ctm@njau.edu.cn。
更新日期/Last Update: 1900-01-01