WU Jingjing,WEI Ziyao,YANG Chunyan,et al.Identification and map-based cloning of OsSET34,a gene controling the delay of heading date in rice(Oryza sativa L).[J].Journal of Nanjing Agricultural University,2015,38(5):704-711.[doi:10.7685/j.issn.1000-2030.2015.05.002]





Identification and map-based cloning of OsSET34,a gene controling the delay of heading date in rice(Oryza sativa L).
吴晶晶 魏子尧 杨春艳 王文艳 朱星洁 刘世家 江玲 万建民
南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心, 江苏 南京 210095
WU Jingjing WEI Ziyao YANG Chunyan WANG Wenyan ZHU Xingjie LIU Shijia JIANG Ling WAN Jianmin
State Key Loboratory of Crop Genetics and Germplasm Enhancement/Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China
riceheading datemutantmap-based cloningmethylation
[目的]抽穗期是影响水稻种植地区、季节适应性及产量的重要农艺性状,受多基因调控,遗传基础复杂.尽管已经进行了大量研究,但仍然有许多未被发掘的控制抽穗期的基因,调控网络还需要进一步完善.[方法]本研究通过60Co-γ辐射诱变水稻品种‘南粳35’,获得1个稳定遗传的抽穗期延迟突变体ah(t).对突变体×野生型F2群体进行遗传分析,利用突变体ב93-11’F2群体(大于10 000株)进行基因定位.[结果]在南京自然长日照条件下,突变体较野生型迟抽穗50 d,在人工短日照条件下,迟抽穗23 d.遗传分析发现:抽穗延迟性状由1对隐性核基因控制,命名为OsSET34.利用定位群体将OsSET34定位在第9染色体长臂物理距离45 kb的分子标记W21和W24之间.利用Gramene和RAP-DB两个网站数据库预测,此区间内分别包含5个和1个开放阅读框(ORF).通过对基因组序列进行分析,发现突变体在ORF1(含SET domain蛋白)编码区的第8外显子上有3个碱基缺失,从而导致第288位的组氨酸变为天冬酰胺,289位的精氨酸缺失,使基因的功能发生改变.[结论]OsSET34基因(SDG724)通过甲基化调控光周期相关基因,进而影响水稻抽穗期.
[Objectives]The heading date in rice is an important agronomic trait that determines the regional adaptability of plants to specific natural environments,growing seasons and yield,which is controlled by polygenes and complicated genetic basis. Vast studies in the past few decades have uncovered a number of flowering-time loci. However,many genes that controlled heading date of rice still should be identified and the regulated pathway awaits in-depth investigations. [Methods]A rice genetically stable heading delay mutant was selected from‘Nanjing 35’ radiated by 60Co-γ radiation. Genetic analysis on the population from the self-cross of F1 hybrid of the wild type×the mutant and based on more than 10 000 individual of F2 population from the mutant and the cultivar‘93-11’. [Results]The mutant displayed heading later than the wild type about 50 days in the normal long-day conditions in Nanjing,even in artificial short-day conditions,also later about 23 days. We found that the heading delay mutant trait was controlled by a single recessive nuclear gene,tentatively designated as OsSET34. OsSET34 was limited in the interval marker W21 and W24 on the long arm of chromosome 9 with the physical range of 45 kb. And,5 and 1 open reading frames(ORF)in this interval were predicted from the data in Gramene and RAP-DB website database,respectively. According to the genome sequences analysis of the wild type and the mutant,there was a deletion of 3 bases in the 8th exon of ORF1(SET domain contain protein)in mutant,which resulted in the 288th amino acid changing from histidine to asparagine and the next arginine missing,and then led to the change of the function of this gene. [Conclusions]The photoperiodic genes were regulated by OsSET34 gene(SDG724)through histone methylation in heading time regulation of rice.


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更新日期/Last Update: 1900-01-01