[1]林玲,伍辉军,梁文伯,等.马来西亚植物根际土壤芽孢杆菌的鉴定及其对水稻白叶枯病防治效果[J].南京农业大学学报,2016,39(3):394-401.[doi:10.7685/jnau.201509009]
 LIN Ling,WU Huijun,LIANG Wenbo,et al.Identification of the Bacillus spp. strains isolated from Malaysia plant rhizosphere soil and their control effects against rice bacterial leaf blight[J].Journal of Nanjing Agricultural University,2016,39(3):394-401.[doi:10.7685/jnau.201509009]
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马来西亚植物根际土壤芽孢杆菌的鉴定及其对水稻白叶枯病防治效果()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年3期
页码:
394-401
栏目:
出版日期:
2016-05-06

文章信息/Info

Title:
Identification of the Bacillus spp. strains isolated from Malaysia plant rhizosphere soil and their control effects against rice bacterial leaf blight
作者:
林玲 伍辉军 梁文伯 高学文
南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095
Author(s):
LIN Ling WU Huijun LIANG Wenbo GAO Xuewen
College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
关键词:
芽孢杆菌16S rDNA 鉴定gyrB基因鉴定抑菌活性脂肽类抗生素生物防治
Keywords:
Bacillus spp.16S rDNA identificationgyrB gene identificationantibacterial activitylipopeptidesbiocontrol
分类号:
Q93-3;S476
DOI:
10.7685/jnau.201509009
摘要:
[目的] 拟从马来西亚植物根际土壤中分离和鉴定抑菌能力强的芽孢杆菌菌株,并对菌株产生的脂肽类抗菌物质的种类以及生防效果进行研究。[方法] 采用平板对峙试验,筛选具有抑制水稻白叶枯病菌和油菜菌核病菌的芽孢杆菌,基于16S rDNA、gyrB基因序列对分离菌株进行分类鉴定,采用基质协助激光解吸附/电离-飞行时间质谱(MALDI-TOF-MS)法鉴定菌株所产生的脂肽类抗生素的种类,并通过温室试验对菌株防治水稻白叶枯病的效果进行研究。[结果] 拮抗试验结果表明:D15、W3、Klu7、YM16、D1和PLZ26这6株菌株对水稻白叶枯病菌或油菜菌核病菌具有较强的抑制活性,其中菌株D15和W3对这2种病原菌都具有抑制活性。基于16S rDNA和gyrB基因的系统发育分析表明:菌株D15和W3为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);菌株Klu7属于短小芽孢杆菌(B. pumilus);菌株YM16属于蜡样芽孢杆菌(B. cereus);菌株D1和PLZ26属于苏云金芽孢杆菌(B. thuringiensis)。MALDI-TOF-MS检测结果表明:菌株PLZ26和Klu7能产生脂肽类抗生素Surfactin;D15和W3能产生脂肽类抗生素Iturin A。温室生防试验结果表明:菌株PLZ26对水稻白叶枯病具有较好的防治效果。[结论] 本研究为生物农药的研发提供了优良的菌株资源。
Abstract:
[Objectives] The main purpose of this study is to screen the Bacillus spp. with high antimicrobial activity from the rhizosphere soil of Malaysia. The lipopeptides produced by the Bacillus strains were identified, and biocontrol activities of these strains were evaluated.[Methods] The analysis of antagonistic activity was used to screen the Bacillus spp. with strong antagonistic activities against Xanthomonas oryzae pv.oryzae or Sclerotinia sclerotiorum. The phylogenetic tree based on the sequences of 16S rDNA and gyrB gene was used to identify the isolated Bacillus spp. strains. The matrix-assisted laser desorption/ionization-time of fligh mass spectrometry(MALDI-TOF-MS)was used to analyze the lipopeptide compound produced by the isolated strains. The greenhouse expriments were performed to evaluate the biocontrol effect on rice bacterial leaf blight of Bacillus spp. strains.[Results] The results of antagonistic activities showed that six isolated Bacillus strains(D15, W3, Klu7, YM16, D1 and PLZ26)have significant inhibitory activities against Xanthomonas oryzae pv.oryzae or Sclerotinia sclerotiorum, and among these strains, D15 and W3 have inhibitory activities to both of them. Phylogenetic tree indicated that these six isolates were identified to be most closely related to Bacillus amyloliquefaciens(D15 and W3), B. pumilus(Klu7), B. cereus(YM16)and B. thuringiensis(D1 and PLZ26). Lipopeptide compound analysis via MALDI-TOF-MS showed that PLZ26 and Klu7 produced Surfactin, and strains D15 and W3 produced Iturin A. The evaluation of the biocontrol effect showed that PLZ26 has good control effect on bacterial blight of rice.[Conclusions] This study provided the bacterial resources for the research and exploitation of biopesticides.

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备注/Memo

备注/Memo:
收稿日期:2015-09-07。
基金项目:中央高校基本科研业务费专项资金(KYZ201404);国家自然科学基金项目(31100056,31471811)
作者简介:林玲,硕士研究生。
通信作者:伍辉军,副教授,硕导,主要研究植物病害生物防治以及细菌分子生物学,E-mail:hjwu@njau.edu.cn。
更新日期/Last Update: 1900-01-01