[1]耿芳芳,许伟,范梦雪,等.脱氧雪腐镰刀菌烯醇对雏鸡脂质过氧化反应及脑部形态结构的影响[J].南京农业大学学报,2016,39(3):460-466.[doi:10.7685/jnau.201510024]
 GENG Fangfang,XU Wei,FAN Mengxue,et al.Effects of deoxynivalenol on lipid peroxidation reaction and brain morphology in chicken[J].Journal of Nanjing Agricultural University,2016,39(3):460-466.[doi:10.7685/jnau.201510024]
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脱氧雪腐镰刀菌烯醇对雏鸡脂质过氧化反应及脑部形态结构的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年3期
页码:
460-466
栏目:
出版日期:
2016-05-06

文章信息/Info

Title:
Effects of deoxynivalenol on lipid peroxidation reaction and brain morphology in chicken
作者:
耿芳芳 许伟 范梦雪 宫佳杰 胡文娟 孟婷婷 李玉 冯士彬 吴金节 王希春
安徽农业大学动物科技学院, 安徽 合肥 230036
Author(s):
GENG Fangfang XU Wei FAN Mengxue GONG Jiajie HU Wenjuan MENG Tingting LI Yu FENG Shibin WU Jinjie WANG Xichun
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
关键词:
脱氧雪腐镰刀菌烯醇雏鸡脂质过氧化
Keywords:
deoxynivalenolchickenbrainlipid peroxidation
分类号:
S816.7
DOI:
10.7685/jnau.201510024
摘要:
[目的] 本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)对雏鸡脑组织形态结构、血清及脑组织中氧化与抗氧化指标的影响,探讨DON的神经毒理作用。[方法] 选取120只健康的1日龄海兰蛋雏公鸡,预饲1周后,按单因素试验设计,随机分成4组,每组30只。对照组和试验组饲喂相同的全价饲料,试验组中的低剂量组、中剂量组和高剂量组按采食量分别添加0.27、1.68和12.21 mg·kg-1剂量的DON灌胃染毒,对照组灌服等量的生理盐水,试验从第8天开始,每隔7 d染毒1次,共染毒5次。试验期为36 d。试验结束时,所有雏鸡经翅下静脉采血,分离血清,用于测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)、一氧化氮(NO)含量等指标;同时每组随机剖杀20只鸡,迅速取脑组织,一部分用于观察脑组织的病理变化及神经细胞的超微结构,另一部分用于测定氧化与抗氧化指标。[结果] 与对照组相比,试验组雏鸡血清CAT、SOD和脑组织SOD、GSH-Px活性及NO含量显著降低(P<0.05),各试验组雏鸡血清MDA和高剂量组脑组织MDA含量显著升高(P<0.05),中剂量组和高剂量组血清T-AOC、GSH-Px和脑组织CAT活性及NO含量显著降低(P<0.05),高剂量组脑组织T-AOC活性显著降低(P<0.05);DON可引起试验组雏鸡脑组织产生不同程度的噬神经现象,并伴随部分神经纤维断裂;大脑皮质超微结构显示,随着染毒剂量的增加,试验组鸡脑组织神经元胞体出现不同程度的变性,且与剂量呈正相关。[结论] DON可引起雏鸡血清及脑组织脂质过氧化反应,造成脑组织不同程度损伤,即DON可对雏鸡产生一定的神经毒性。
Abstract:
[Objectives] The aims of this experiment was to study the nerve toxicological effects of chicken induced by deoxynivalenol(DON), through the changes of brain issue morphology and the indexes of oxidase and antioxidase in sera and brain issues of chicken.[Methods] After feeding a week, 120 Hailan chickens(male, 1 day old)were selected and randomly divided into four groups and 30 chickens of each, according to single factor experiment design. Chickens in control and treatment groups were fed with the same full price diets. Chicken in low, medium and high dose groups were orally gavaged with DON solution with 0.27, 1.68 and 12.21 mg·kg-1 according to the feed intake, respectively. Chickens in control group were orally gavaged with the same dose of sodium chloride solution. The trial was conducted for 36 days. All chickens in treatment group were gavaged with DON once every seven day, a total of 5 times. At the end of the trial, the blood samples were collected via wing vein and the sera were separated for measuring activities of catalase(CAT), superoxide dismutase(SOD), total antioxidant capacity(T-AOC), glutathione peroxidase(GSH-Px), and contents of malondialdehyde(MDA), nitric oxide(NO). At the same time, 20 chickens from each group were slaughtered, and the brain tissues were collected immediately, one part was used to observe the changes of pathology and ultrastructure, the other part was used to measure the indexes of oxidase and antioxidase.[Results] Compared with the control group, the activities of CAT, NO, SOD in sera and SOD, GSH-Px in brain tissues significantly decreased(P<0.05)in treatment groups, the contents of serum MDA in three treatment groups and brain tissue MDA in high dose group significantly increased(P<0.05), the activities of serum T-AOC, GSH-Px and brain tissue CAT, and the contents of NO in medium and high dose groups significantly decreased(P<0.05), and the activities of brain T-AOC in high dose group significantly decreased(P<0.05). DON could trigger the neuronophagia with varying degrees in treatment groups, accompanied with part of the nerve fiber breakage. The degrees of degeneration in neurons cell increased with increasing DON concentration through the ultrastructure of cerebral cortex.[Conclusions] DON can cause the lipid peroxidation reaction in serum and brain tissue, resulting in varying degrees of brain damage in chickens. It suggests that DON has some neurotoxic effects to the chicken.

参考文献/References:

[1] Hussein S H,Jeffrey M B. Toxicity,metabolism,and impact of mycotoxins on humans and animals[J]. Toxicology,2001,167(2):101-134.
[2] Ren Z H,Wang Y C,Deng H D,et al. Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway[J]. Environmental Toxicology and Pharmacology,2015,39(1):339-346.
[3] Ebrahem M,Kersten S,Valenta H,et al. Effects of feeding deoxynivalenol(DON)-contaminated wheat to laying hens and roosters of different genetic background on the reproductive performance and health of the newly hatched chicks[J]. Mycotoxin Research,2014,30(3):131-140.
[4] Faixova Z,Faix S,Borutova R,et al. Efficacy of dietary selenium to counteract toxicity of deoxynivalenol in growing broiler chickens[J]. Acta Veterinaria Brno,2007,76(3):349-356.
[5] Prelusky D B,Yeung J M,Thompson B K,et al. Effeck of deoxynivalenol on neurotransmitters in discrete regions of swine brain[J]. Archives of Environmental Contamination and Toxicology,1992,22(3):36-40.
[6] Ghareeb K,Awad W A,Sid-Ahmed O E. Insights on the host stress,fear and growth responses to the deoxynivalenol feed contaminant in broiler chickens[J]. PLoS ONE,2014,9(1):1-7.
[7] Swamy H V L N,Smith T K,MacDonald E J. Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on brain regional neurochemistry of starter pigs and broiler chickens[J]. Journal of Animal Science,2004,82(7):2131-2139.
[8] Girish C K,MacDonald E J,Scheinin M,et al. Effects of feedborne Fusarium mycotoxins on brain regional neurochemistry of turkeys[J]. Poultry Science,2008,87(7):1295-1302.
[9] 李荣佳,李治忠,周闯,等. 新型复合吸附剂HG对黄曲霉毒素B1和呕吐毒素的吸附脱毒研究[J]. 南京农业大学学报,2015,38(1):113-119. DOI:10.7685/j.issn.1000-2030.2015.01.017. Li R J,Li Z Z,Zhou C,et al. Detoxification of aflatoxin B1 and deoxynivalenol by new compound adsorbent HG[J]. Journal of Nanjing Agricultural University,2015,38(1):113-119(in Chinese with English abstract).
[10] Borutova R,Faix S,Placha I,et al. Effects of deoxynivalenol and zearalenone on oxidative stress and blood phagocytic activity in broilers[J]. Archives of Animal Nutrition,2008,62(4):303-312.
[11] Krishnaswamy R,Devaraj S N,Padma V V. Lutein protects HT-29 cells against deoxynivalenol-induced oxidative stress and apoptosis:prevention of NF-κB nuclear localization and down regulation of NF-κB and cyclo-oxygenase-2 expression[J]. Free Radical Biology and Medicine,2010,49(1):50-60.
[12] Yunus A W,Ghareeb K,Twaruzek M,et al. Deoxynivalenol as a contaminant of broiler feed:effects on bird performance and response to common vaccines[J]. Poultry Science,2012,91(4):844-851.
[13] 蒋竹英,李丽立,唐利华,等. DON污染饲粮添加竹炭和竹醋液对断奶仔猪抗氧化性能及小肠黏膜形态的影响[J]. 西北农林科技大学学报(自然科学版),2013,41(12):37-42. Jiang Z Y,Li L L,Tang L H,et al. Effects of adding bamboo charcoal and bamboo vinegar to DON contaminated diets on oxidation resistance and small intestinal morphology of weaned piglets[J]. Journal of Northwest A&F University(Natural Science Edition),2013,41(12):37-42(in Chinese with English abstract).
[14] Pestka J J,Zhou H R,Moon Y,et al. Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes:unraveling a paradox[J]. Toxicology Letters,2004,153(1):61-73.
[15] Kouadio J H,Mobio T A,Baudrimont I,et al. Comparative study of cytotoxicity and oxidative stress induced by deoxynivalenol,zearalenone or fumonisin B1 in human intestinal cell line Caco-2[J]. Toxicology,2005,213(1/2):56-65.
[16] 李洋. 镰刀菌毒素ZEA、DON及其联合染毒对小鼠脑损伤机制的研究[D]. 雅安:四川农业大学,2013. Li Y. The research on injury mechanism of deoxynivalenol,zearalenone and co-infection on brain of mice[D]. Ya’an:Sichun Agricultural University,2013(in Chinese with English abstract).
[17] Ledo A,Frade J,Barbosa R M,et al. Nitric oxide in brain:diffusion,targets and concentration dynamics in hippocampal subregions[J]. Molecular Aspects of Medicine,2004,25(1/2):75-89.
[18] Awad W A,Hess M,Twaruzek M,et al. The impact of the Fusarium mycotoxin deoxynivalenol on the health and performance of broiler chickens[J]. International Journal of Molecular Sciences,2011,12(11):7996-8012.
[19] Anagli J,Abounit K,Stemmer P,et al. Effects of cathepsins B and L inhibition on postischemic protein alterations in the brain[J]. Biochemical and Biophysical Research Communications,2008,366(1):86-91.
[20] Yamashima T,Oikawa S. The role of lysosomal rupture in neuronal death[J]. Progress in Neurobiology,2009,89(4):343-358.

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备注/Memo

备注/Memo:
收稿日期:2015-10-18。
基金项目:国家自然科学基金项目(31472250);安徽省教育厅重点资助项目(KJ2013A119)
作者简介:耿芳芳,硕士研究生。
通信作者:王希春,博士,副教授,硕导,研究方向为畜禽营养代谢病与中毒病,E-mail:wangxichun@ahau.edu.cn。
更新日期/Last Update: 1900-01-01