LIU Xiaodong,LI Yue,WANG Ruozhong,et al.Molecular mechanism of drought tolerance conferred by overexpression of GH3-5[J].Journal of Nanjing Agricultural University,2016,39(4):557-562.[doi:10.7685/jnau.201604019]





Molecular mechanism of drought tolerance conferred by overexpression of GH3-5
刘晓东1 李月1 王若仲2 代培红1 刘超1 石书兵1
1. 新疆农业大学农学院/农业生物技术重点实验室, 新疆 乌鲁木齐 830052;
2. 湖南农业大学植物激素与生长发育湖南省重点实验室, 湖南 长沙 410128
LIU Xiaodong1 LI Yue1 WANG Ruozhong2 DAI Peihong1 LIU Chao1 SHI Shubing1
1. Key Laboratory of Agricultural Biological Technology/College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
GH3drought tolerancesalicylic acidauxin
[目的] 拟南芥GH3-5GH3-6基因属于生长素早期应答基因GH3基因家族。GH3-5基因过表达植株gh3.5-1DGH3-6基因过表达植株dfl1-D都表现出生长素响应缺失的表型,然而与野生型对照相比两者的抗旱能力却完全相反。研究GH3-5基因的抗旱机制,将能解释两者抗旱表型完全相反的原因。[方法] 采用液相色谱-质谱联用技术测定了干旱胁迫后gh3.5-1Ddfl1-D及其对应野生型中水杨酸的含量,同时检测了gh3.5-1D/NahGgh3.5-1D/npr1两种双突变体的抗旱性。[结果] 与野生型相比,干旱胁迫后dfl1-D中水杨酸(SA)的含量没有差异,而gh3.5-1D中SA的含量增加了1倍。进一步研究发现,gh3.5-1D较高的抗旱能力在gh3.5-1D/NahG双突变体中丧失,而在gh3.5-1D/npr1双突变体中没有明显变化。[结论] 水杨酸的过量积累是gh3.5-1D抗旱性发生逆转的原因,然而这种SA赋予的抗旱性可能并不依赖NPR1。
[Objectives] GH3-5 and GH3-6 belong to the same gene family of GH3 that are early auxin-responsive genes in Arabidopsis. Both of gh3.5-1D and dfl1-D,the over expression lines of GH3-5 and GH3-6 respectively,exhibit similar auxin-related defects. However,their phenotypes of drought tolerance are completely opposite compared to wildtype. Here we explored the underlying mechanism of drought tolerance conferred by overexpression of GH3-5 and explained the difference of drought response between gh3.5-1D and dfl1-D.[Methods] The level of salicylic acid (SA) was measured by liquid chromatography-mass spectrometry. Drought tolerance was tested in SA deficient and insensitive background using gh3.5-1D/NahG and gh3.5-1D/npr1.[Results] We found that SA levels before and after drought stress in dfl1-D were comparable to those in the wild-type respectively. In contrast,we found that gh3.5-1D accumulated one more fold of SA than the corresponding wild types after drought stress. Further study found that drought tolerance in gh3.5-1D was compromised in gh3.5-1D/NahG but not in gh3.5-1D/npr1.[Conclusions] Drought tolerance conferred by overexpression of GH3.5 depends on NPR1-independent salicylic acid signaling pathway.


[1] Wang R,Estelle M. Diversity and specificity:auxin perception and signaling through the TIR1/AFB pathway[J]. Current Opinion in Plant Biology,2014,21:51-58.
[2] Shi H,Chen L,Ye T,et al. Modulation of auxin content in Arabidopsis confers improved drought stress resistance[J]. Plant Physiology and Biochemistry,2014,82:209-217.
[3] Kim J I,Baek D,Park H C,et al. Overexpression of Arabidopsis YUCCA6 in potato results in high-auxin developmental phenotypes and enhanced resistance to water deficit[J]. Molecular Plant,2013,6(2):337-349.
[4] Hagen G,Guilfoyle T. Auxin-responsive gene expression:genes,promoters and regulatory factors[J]. Plant Molecular Biology,2002,49(3/4):373-385.
[5] Staswick P E,Tiryaki I,Rowe M L. Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic,salicylic,and indole-3-acetic acids in an assay for adenylation[J]. The Plant Cell,2002,14(6):1405-1415.
[6] Chang K H,Xiang H,Dunaway-Mariano D. Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily:a site-directed mutagenesis study with the Pseudomonas sp. strain CBS34-chlorobenzoate:coenzyme A ligase[J]. Biochemistry,1997,36(50):15650-15659.
[7] Park J E,Park J Y,Kim Y S,et al. GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis[J]. Journal of Biological Chemistry,2007,282(13):10036-10046.
[8] Singh V K,Jain M,Garg R. Genome-wide analysis and expression profiling suggest diverse roles of GH3 genes during development and abiotic stress responses in legumes[J]. Frontiers in Plant Science,2015,5:789.
[9] Feng S,Yue R,Tao S,et al. Genome-wide identification,expression analysis of auxin-responsive GH3 family genes in maize(Zea mays L.)under abiotic stresses[J]. Journal of Integrative Plant Biology,2015,57(9):783-795.
[10] Zhang S W,Li C H,Cao J,et al. Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation[J]. Plant Physiology,2009,151(4):1889-1901.
[11] Kumar D. Salicylic acid signaling in disease resistance[J]. Plant Science,2014,228:127-134.
[12] Nazar R,Umar S,Khan N A. Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress[J]. Plant Signaling and Behavior,2015,10(3):e1003751.
[13] Naser Alavi S M,Arvin M J,Kalantari K M. Salicylic acid and nitric oxide alleviate osmotic stress in wheat(Triticum aestivum L.)seedlings[J]. Journal of Plant Interactions,2014,9(1):683-688.
[14] Fayez K A,Bazaid S A. Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate[J]. Journal of the Saudi Society of Agricultural Sciences,2014,13(1):45-55.
[15] Khan M I,Iqbal N,Masood A,et al. Salicylic acid alleviates adverse effects of heat stress on photosynthesis through changes in proline production and ethylene formation[J]. Plant Signaling and Behavior,2013,8(11):e26374.
[16] Wildermuth M C,Dewdney J,Wu G,et al. Isochorismate synthase is required to synthesize salicylic acid for plant defence[J]. Nature,2001,414(6863):562-565.
[17] Gao Q M,Zhu S,Kachroo P,et al. Signal regulators of systemic acquired resistance[J]. Frontiers in Plant Science,2015,6:228.
[18] Janda M,Ruelland E. Magical mystery tour:salicylic acid signalling[J]. Environmental and Experimental Botany,2015,114:117-128.
[19] Nakazawa M,Yabe N,Ichikawa T,et al. DFL1,an auxin-responsive GH3 gene homologue,negatively regulates shoot cell elongation and lateral root formation,and positively regulates the light response of hypocotyl length[J]. The Plant Journal,2001,25(2):213-221.
[20] Zhang Z Q,Li Q,Li Z M,et al. Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction[J]. Plant Physiology,2007,145(2):450-464.
[21] Zhang Z,Wang M,Li Z,et al. Arabidopsis GH3.5 regulates salicylic acid-dependent and both NPR1-dependent and independent defense responses[J]. Plant Signaling and Behavior,2008,3(8):537-542.
[22] 王若仲,萧浪涛,蔺万煌,等. 亚种间杂交稻内源激素的高效液相色谱测定法[J]. 色谱,2002,20(2):148-150. Wang R Z,Xiao L T,Lin W H,et al. High performance liquid chromatographic determination of internal hormones in inter-subspecific hybrid rice[J]. Chinese Journal of Chromatography,2002,20(2):148-150(in Chinese with English abstract).
[23] Miura K,Okamoto H,Okuma E,et al. SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis[J]. The Plant Journal,2013,73(1):91-104.
[24] Okuma E,Nozawa R,Murata Y,et al. Accumulation of endogenous salicylic acid confers drought tolerance to Arabidopsis[J]. Plant Signaling and Behavior,2014,9(3):e28085.
[25] Fragniere C,Serrano M,Abou-Mansour E,et al. Salicylic acid and its location in response to biotic and abiotic stress[J]. FEBS Letters,2011,585(12):1847-1852.
[26] Kraft M,Kuglitsch R,Kwiatkowski J,et al. Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid dioxygenase gene expression and abscisic acid accumulation in cleavers(Galium aparine):interaction with ethylene[J]. Journal of Experimental Botany,2007,58(6):1497-1503.
[27] Bandurska H,Stroinski A. The effect of salicylic acid on barley response to water deficit[J]. Acta Physiologiae Plantarum,2005,27(3):379-386.
[28] Szepesi Á,Csiszar J,Gemes K,et al. Salicylic acid improves acclimation to salt stress by stimulating abscisic aldehyde oxidase activity and abscisic acid accumulation,and increases Na+ content in leaves without toxicity symptoms in Solanum lycopersicum L.[J]. Journal of Plant Physiology,2009,166(9):914-925.
[29] Du H,Wu N,Fu J,et al. A GH3 family member,OsGH3-2,modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice[J]. Journal of Experimental Botany,2012,63(18):6467-6480.
[30] Jayakannan M,Bose J,Babourina O,et al. The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis[J]. Journal of Experimental Botany,2015,66(7):1865-1875.


 SUN Jing,ZENG Jun,WANG Yinjie,et al.Evaluation and screening of drought stress tolerance in 20 cut chrysanthemum varieties[J].Journal of Nanjing Agricultural University,2013,36(4):24.[doi:10.7685/j.issn.1000-2030.2013.01.005]


更新日期/Last Update: 1900-01-01