[1]刘晓东,李月,王若仲,等.过表达GH3-5提高拟南芥抗旱的分子机制[J].南京农业大学学报,2016,39(4):557-562.[doi:10.7685/jnau.201604019]
 LIU Xiaodong,LI Yue,WANG Ruozhong,et al.Molecular mechanism of drought tolerance conferred by overexpression of GH3-5[J].Journal of Nanjing Agricultural University,2016,39(4):557-562.[doi:10.7685/jnau.201604019]
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过表达GH3-5提高拟南芥抗旱的分子机制()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年4期
页码:
557-562
栏目:
出版日期:
2016-07-01

文章信息/Info

Title:
Molecular mechanism of drought tolerance conferred by overexpression of GH3-5
作者:
刘晓东1 李月1 王若仲2 代培红1 刘超1 石书兵1
1. 新疆农业大学农学院/农业生物技术重点实验室, 新疆 乌鲁木齐 830052;
2. 湖南农业大学植物激素与生长发育湖南省重点实验室, 湖南 长沙 410128
Author(s):
LIU Xiaodong1 LI Yue1 WANG Ruozhong2 DAI Peihong1 LIU Chao1 SHI Shubing1
1. Key Laboratory of Agricultural Biological Technology/College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
关键词:
GH3抗旱性水杨酸生长素
Keywords:
GH3drought tolerancesalicylic acidauxin
分类号:
Q37
DOI:
10.7685/jnau.201604019
摘要:
[目的] 拟南芥GH3-5GH3-6基因属于生长素早期应答基因GH3基因家族。GH3-5基因过表达植株gh3.5-1DGH3-6基因过表达植株dfl1-D都表现出生长素响应缺失的表型,然而与野生型对照相比两者的抗旱能力却完全相反。研究GH3-5基因的抗旱机制,将能解释两者抗旱表型完全相反的原因。[方法] 采用液相色谱-质谱联用技术测定了干旱胁迫后gh3.5-1Ddfl1-D及其对应野生型中水杨酸的含量,同时检测了gh3.5-1D/NahGgh3.5-1D/npr1两种双突变体的抗旱性。[结果] 与野生型相比,干旱胁迫后dfl1-D中水杨酸(SA)的含量没有差异,而gh3.5-1D中SA的含量增加了1倍。进一步研究发现,gh3.5-1D较高的抗旱能力在gh3.5-1D/NahG双突变体中丧失,而在gh3.5-1D/npr1双突变体中没有明显变化。[结论] 水杨酸的过量积累是gh3.5-1D抗旱性发生逆转的原因,然而这种SA赋予的抗旱性可能并不依赖NPR1。
Abstract:
[Objectives] GH3-5 and GH3-6 belong to the same gene family of GH3 that are early auxin-responsive genes in Arabidopsis. Both of gh3.5-1D and dfl1-D,the over expression lines of GH3-5 and GH3-6 respectively,exhibit similar auxin-related defects. However,their phenotypes of drought tolerance are completely opposite compared to wildtype. Here we explored the underlying mechanism of drought tolerance conferred by overexpression of GH3-5 and explained the difference of drought response between gh3.5-1D and dfl1-D.[Methods] The level of salicylic acid (SA) was measured by liquid chromatography-mass spectrometry. Drought tolerance was tested in SA deficient and insensitive background using gh3.5-1D/NahG and gh3.5-1D/npr1.[Results] We found that SA levels before and after drought stress in dfl1-D were comparable to those in the wild-type respectively. In contrast,we found that gh3.5-1D accumulated one more fold of SA than the corresponding wild types after drought stress. Further study found that drought tolerance in gh3.5-1D was compromised in gh3.5-1D/NahG but not in gh3.5-1D/npr1.[Conclusions] Drought tolerance conferred by overexpression of GH3.5 depends on NPR1-independent salicylic acid signaling pathway.

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备注/Memo

备注/Memo:
收稿日期:2016-04-07。
基金项目:国家自然科学基金项目(31470289)
作者简介:刘晓东,副教授,研究方向为植物抗逆分子机制,E-mail:xiaodongliu75@aliyun.com。
通信作者:石书兵,教授,主要从事植物抗性机制研究,E-mail:shbshi@sina.com。
更新日期/Last Update: 1900-01-01