[1]张叶秋,郝帅帅,高硕,等.米糠高纤维日粮对苏淮猪生长性能及肠道功能的影响[J].南京农业大学学报,2016,39(5):807-813.[doi:10.7685/jnau.201601011]
 ZHANG Yeqiu,HAO Shuaishuai,GAO Shuo,et al.Effects of rice bran source high fibre diet on growth performance and intestine function of Suhuai pigs[J].Journal of Nanjing Agricultural University,2016,39(5):807-813.[doi:10.7685/jnau.201601011]
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米糠高纤维日粮对苏淮猪生长性能及肠道功能的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年5期
页码:
807-813
栏目:
出版日期:
2016-09-16

文章信息/Info

Title:
Effects of rice bran source high fibre diet on growth performance and intestine function of Suhuai pigs
作者:
张叶秋12 郝帅帅12 高硕12 吴艳13 李强3 李平华12 黄瑞华12
1. 南京农业大学养猪研究所, 江苏 南京 210095;
2. 南京农业大学淮安研究院, 江苏 淮安 223005;
3. 淮安市淮阴种猪场, 江苏 淮安 223322
Author(s):
ZHANG Yeqiu12 HAO Shuaishuai12 GAO Shuo12 WU Yan13 LI Qiang3 LI Pinghua12 HUANG Ruihua12
1. Institute of Swine Science, Nanjing Agricultural University, Nanjing 210095, China;
2. Huai’an Academy of Nanjing Agricultural University, Huai’an 223005, China;
3. Huaiyin Pig Breeding Farm of Huai’an City, Huai’an 223322, China
关键词:
米糠纤维苏淮猪生长性能肠道发育小肠形态肠道微生物
Keywords:
rice brandietary fibreSuhuai pigsgrowth performanceintestinal developmentintestinal morphologygut microbiota
分类号:
S828
DOI:
10.7685/jnau.201601011
摘要:
[目的] 本试验旨在研究米糠替代部分玉米形成的高纤维日粮对我国培育新品种苏淮猪生长性能、小肠形态、肠道发育及微生物区系的影响。[方法] 试验选取160日龄左右、体质量相近的纯种苏淮猪24头,随机分为2组,分别饲喂粗纤维含量为4.7%的玉米-豆粕型基础日粮和粗纤维含量为7.5%的米糠替代部分玉米的高纤维日粮,试验期75 d。分别于试验第0、14、28和75天采集粪样,用于测定羧甲基纤维素酶活性和微生物数量。试验结束屠宰全部试验猪,测量胃肠道相对质量和相对长度,并采集小肠组织样,用于形态学观察。[结果] 与对照组相比,米糠组平均上午采食量和日均采食量极显著下降(P<0.01),平均日增重与料重比无显著变化(P>0.05)。试验第14天,米糠组羧甲基纤维素酶活性极显著升高(P<0.01),黄色瘤胃球菌数量增加(P<0.05);乳酸杆菌和双歧杆菌数量在试验第28天均显著增加(P<0.05);柔嫩梭菌数量在试验结束时显著升高(P<0.05),而白色瘤胃球菌在整个试验期均无显著变化(P>0.05)。饲喂米糠增加了大肠占整个肠道质量的比例(P<0.01),同时促进了十二指肠和空肠绒毛生长(P<0.01),加深了空肠隐窝深度(P<0.05)。[结论] 添加34.8%米糠的高纤维日粮不影响苏淮猪(50~90 kg)生长,并能促进小肠绒毛生长,优化大肠微生物区系。
Abstract:
[Objectives] The study was conducted to examine the effects of replacing partial corn with rice bran on growth performance,intestinal morphology,intestinal development and gut microbiota of Chinese new breed Suhuai pigs. [Methods] Twenty-four 160 day-old healthy Suhuai pigs with similar body weight were randomly allocated into 2 groups to receive basal corn-soybean diet containing 4.7% fibre and high fibrerice bran diet containing 7.5% fibre for 75 days,respectively. We collected feces on 0,14,28 and 75 days for caboxymethyl cellulose(CMC) and microbiota analysis. At the end of trial,all pigs were slaughtered and relative weight and length of gastrointestinal tract(GIT) were measured,and small intestine tissues were collected for morphologic observation. [Results] Average feed intake in the morning and average daily feed intake significantly decreased when pigs was fed with rice bran diets,and no significant difference was observed with average daily gain and feed to gain ratio(P>0.05). On 14 days of trial,rice bran diets increased CMC activity(P<0.01) and the number of Ruminococcus flavefaciens(P<0.05). The amount of Lactobacillus spp.and Bifidobacterium increased of pigs with rice bran diets on 28 days of trial(P<0.05). Clostridium leptum population of rice bran diet increased on 75 days of trial(P<0.05),and the number of Ruminococcus albus had no significant changes during the whole trial(P>0.05). Partially replacing of corn by rice bran increased large intestine weight ratio(P<0.05),as well as the duodenal and jejunal villus heights(P<0.01),crypt depths of jejunum(P<0.05). [Conclusions] Supplementation with 34.8% rice bran had no influence on growth performance of Suhuai pigs(50-90 kg),but improved small intestine villus heights,and had optimized effects on microbial community of large intestine.

参考文献/References:

[1] Noblet J,Goff G L. Effect of dietary fibre on the energy value of feeds for pigs[J]. Animal Feed Science and Technology,2001,90(1):35-52.
[2] Simpson H L,Campbell B J. Review article:dietary fibre-microbiotainteractions[J]. Alimentary Pharmacology and Therapeutics,2015,42(2):158-179.
[3] Henderson A J,Ollila C A,Kumar A,et al. Chemopreventive properties of dietary rice bran:current status and future prospects[J]. Advances in Nutrition,2012,3(5):643-653.
[4] Henderson A J,Kumar A,Barnett B,et al. Consumption of rice bran increases mucosal immunoglobulin A concentrations and numbers of intestinal Lactobacillus spp.[J]. Journal of Medicinal Food,2012,15(5):469-475.
[5] Herfel T,Jacobi S,Lin X,et al. Stabilized rice bran improves weaning pig performance via a prebiotic mechanism[J]. Journal of Animal Science,2013,91(2):907-913.
[6] Fevrier C,Bourdon D,Aumaitre A. Effects of level of dietary fibre from wheat bran on digestibility of nutrients,digestive enzymes and performance in the european Large White and Chinese Meishan pig[J]. Journal of Animal Physiology and Animal Nutrition,1992,68(2):60-72.
[7] Kemp B,Hartog L A,Klok J J,et al. The digestibility of nutrients,energy and nitrogen in the Meishan and Dutch Landrace pig[J]. Journal of Animal Physiology and Animal Nutrition,1991,65(1/2/3/4/5):263-266.
[8] 金巍. 草食动物厌氧真菌及其共存甲烷菌的分离鉴定和体外发酵特性的初步研究[D]. 南京:南京农业大学,2009. Jin W. Isolation and identification of anaerobic function and their associated methanogens from herbivorous animals and their characteristics of in vitro fermentation[D]. Nanjing:Nanjing Agricultural University,2009(in Chinese with English abstract).
[9] Yang S L,Bu D P,Wang J Q,et al. Soybean oil and linseed oil supplementation affect profiles of ruminal microorganisms in dairy cows[J]. Animal,2009,3(11):1562-1569.
[10] Tajima K,Aminov R,Nagamine T,et al. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR[J]. Applied and Environmental Microbiology,2001,67(6):2766-2774.
[11] Furet J P,Firmesse O,GourmelonM,et al. Comparative assessment of human and farm animal faecalmicrobiota using real-time quantitative PCR[J]. Fems Microbiology Ecology,2009,68(3):351-362.
[12] Castillo M,Martín-Orúe S M,Manzanilla E G,et al. Quantification of total bacteria,enterobacteria and lactobacilli populations in pig digesta by real-time PCR[J]. Veterinary Microbiology,2006,114(1):165-170.
[13] Len N,Hong T,Ogle B,et al. Comparison of total tract digestibility,development of visceral organs and digestive tract of Mongcai and Yorkshire×Landrace piglets fed diets with different fibre sources[J]. Journal of Animal Physiology and Animal Nutrition,2009,93(2):181-191.
[14] Herfel T,Jacobi S,Lin X,et al. Stabilized rice bran improves weaning pig performance via a prebiotic mechanism[J]. Journal of Animal Science,2013,91(2):907-913.
[15] 李晶,周勤飞,何胜强,等. 米糠和抗氧化剂对肥育猪生产性能和胴体品质的影响[J]. 饲料工业,2009,30(24):45-47. Li J,Zhou Q F,He S Q,et al. The effect of dietary rice bran and antioxidant on performance and carcass traits in fattening pigs[J]. Journal of Animal Physiology and Animal Nutrition Feed Industry,2009,30(24):45-47(in Chinese).
[16] Wenk C. The role of dietary fibre in the digestive physiology of the pig[J]. Animal Feed Science and Technology,2001,90(1):21-33.
[17] Noblet J,Fortune H,Shi X S,et al. Prediction of net energy value of feeds for growing pigs[J]. Journal of Animal Science,1994,72(2):344-354.
[18] Freire J,Guerreiro A,Cunha L,et al. Effect of dietary fibre source on total tract digestibility,caecum volatile fatty acids and digestive transit time in the weaned piglet[J]. Animal Feed Science and Technology,2000,87(1):71-83.
[19] Jørgensen H,Zhao X Q,Eggum B O. The influence of dietary fibre and environmental temoperature on the development of the gastrointestinal tract,digestibility,degree of fermentation in the hind-gut and energy metabolism in pigs[J]. British Journal of Nutrition,1996,75(3):365-378.
[20] Ngoc T T,Hong T T,Len N T,et al. Effect of fibre level and fibre source on gut morphology and micro-environment in local(MongCai) and exotic(Landrace×Yorkshire)pigs[J]. Asian-Australasian Journal of Animal Sciences,2012,25(12):1726-1733.
[21] Chen H,Mao X,Che L,et al. Impact of fiber types on gut microbiota,gut environment and gut function in fattening pigs[J]. Animal Feed Science and Technology,2014,195:101-111.
[22] Jin L,Reynolds L P,Redmer D A,et al. Effects of dietary fiber on intestinal growth,cell proliferation,and morphology in growing pigs[J]. Journal of Animal Science,1994,72(9):2270-2278.
[23] Goodlad R A,Levi S,Lee C Y,et al. Morphometry and cell proliferation in endoscopic biopsies:evaluation of a technique[J]. Gastroenterology,1991,101(5):1235-1241.
[24] Rojo R,Mendoza G,González S,et al. Effects of exogenous amylases from Bacillus licheniformis and Aspergillus niger on ruminal starch digestion and lamb performance[J]. Animal Feed Science and Technology,2005,123:655-665.
[25] Varel V,Yen J T. Microbial perspective on fiber utilization by swine[J]. Journal of Animal Science,1997,75(10):2715-2722.
[26] Weber T E,Ziemer C J,Kerr B J. Effects of adding fibrous feedstuffs to the diet of young pigs on growth performance,intestinal cytokines,and circulating acute-phase proteins[J]. Journal of Animal Science,2008,86(4):871-881.
[27] Whitney M H,Shurson G C,Johnston L J,et al. Growth performance and carcass characteristics of grower-finisher pigs fed high-quality corn distillers dried grain with solubles originating from a modern Midwestern ethanol plant[J]. Journal of Animal Science,2006,84(12):3356-3363.
[28] Liu H,Ivarsson E,Dicksved J,et al. Inclusion of chicory(Cichorium intybus L.)in pigs’ diets affects the intestinal microenvironment and the gut microbiota[J]. Applied and Environmental Microbiology,2012,78(12):4102-4109.
[29] 冯平. 肥育猪对不同类型纤维消化适应性及其后肠发酵与微生物菌群变化研究[D]. 北京:中国农业科学院,2013. Feng P. Adaptation to different types of fiber in growing-finishing swine and changes of hindgut fermentation and microbiota[D]. Beijing:Chinese Academy of Agricultural Sciences,2013(in Chinese with English abstract).
[30] Pryde S E,Duncan S H,Hold G L,et al. The microbiology of butyrate formation in the human colon[J]. Femsmicrobiology Letters,2002,217(2):133-139.
[31] Chen H,Mao X,He J,et al. Dietary fibre affects intestinal mucosal barrier function and regulates intestinal bacteria in weaning piglets[J]. British Journal of Nutrition,2013,110(10):1837-1848.

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备注/Memo

备注/Memo:
收稿日期:2016-01-10
基金项目:国家科技支撑计划项目(2015BAD03B02-4);江苏省农业科技自主创新项目(CX(15)1006);中央高校基本业务费(KYCYL201502);淮安市科技支撑计划(农业)项目(SN13094)
作者简介:张叶秋,硕士研究生
通信作者:黄瑞华,教授,研究方向为猪健康生产与遗传育种,E-mail:hrh2002@163.com。
更新日期/Last Update: 1900-01-01