[1]马百全,魏天雨,吴艳,等.苏淮猪BMP4基因3’-UTR区克隆和多态性分析[J].南京农业大学学报,2016,39(5):819-824.[doi:10.7685/jnau.201602002]
 MA Baiquan,WEI Tianyu,WU Yan,et al.Cloning and polymorphism of BMP4 gene 3’-UTR in Suhuai pig[J].Journal of Nanjing Agricultural University,2016,39(5):819-824.[doi:10.7685/jnau.201602002]
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苏淮猪BMP4基因3’-UTR区克隆和多态性分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年5期
页码:
819-824
栏目:
出版日期:
2016-09-16

文章信息/Info

Title:
Cloning and polymorphism of BMP4 gene 3’-UTR in Suhuai pig
作者:
马百全1 魏天雨1 吴艳2 郭苹3 王钧顺2 张建生3 邢军3 刘红林1 李齐发1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 淮阴种猪场, 江苏 淮安 223322;
3. 江苏农林职业技术学院, 江苏 句容 212400
Author(s):
MA Baiquan1 WEI Tianyu1 WU Yan2 GUO Ping3 WANG Junshun2 ZHANG Jiansheng3 XING Jun3 LIU Honglin1 LI Qifa1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Huaiyin Pig Breeding Farm, Huai’an 223322, China;
3. Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China
关键词:
苏淮猪BMP4基因3’-UTRSNP多态性
Keywords:
Suhuai pigBMP4 gene3’-UTRSNPpolymorphism
分类号:
S828.8
DOI:
10.7685/jnau.201602002
摘要:
[目的] 本试验旨在研究梅山猪和苏淮猪BMP4(bone morphogenetic protein 4)基因3’-UTR区序列特征,分析苏淮猪BMP4基因3’-UTR区SNP位点并了解苏淮猪和高繁殖力猪种梅山猪BMP4基因3’-UTR SNP位点多态性。[方法] 采用克隆测序技术获得苏淮猪BMP4基因3’-UTR区序列;利用生物信息学方法分析其序列特征;利用池DNA测序技术筛选SNPs,采用AS-PCR技术分析苏淮猪和梅山猪SNPs位点多态性。[结果] 苏淮猪BMP4基因3’-UTR区序列长度为267 bp,含有一个(AC)9微卫星位点和PolyA结构;在苏淮猪BMP4基因3’-UTR区20 nt处发现一个SNP,AS-PCR检测到3种基因型(GG、GC和CC);在苏淮猪中,GG型和G等位基因为优势基因型和优势等位基因,频率分别为0.815和0.887,而在高繁殖力猪种梅山猪中CC型和C等位基因为优势基因型和优势等位基因,频率分别为为0.667和0.770。[结论] 获得了BMP4基因3’-UTR区序列,鉴定了一个新的SNP位点,发现BMP4基因3’-UTR区20G>C位点多态性可能与猪繁殖性能有关。
Abstract:
[Objectives] The objective of this study is to identify the characterization of 3’-UTR sequence of Suhuai pig bone morphogenetic protein 4(BMP4) gene and the polymorphism of SNPs in 3’-UTR of BMP4 between Suhuai pig and the high reproduction of Meishan pig. [Methods] The 3’-UTR sequence of BMP4 was obtained by the cloning and sequencing and analyzed using bioinformatics methods. The SNPs of BMP4 were screened by sequencing of DNA pool and detected by the AS-PCR method between Suhuai pig and Meishan pig. [Results] The length of 3’-UTR region of Suhuai pig BMP4 gene was 267 bp and contained a microsatellite loci of(AC) 9. Moreover,we found an SNP in the 20 nt in the 3’-UTR of Suhuai pig BMP4 gene and detected three genotypes of GG,GC and CC by AS-PCR. The genotype of GG and G allele were higher in Suhuai pig and their frequency were 0.815 and 0.887,respectively. However,the genotype of CC and C allele were higher in high reproduction of Meishan pig and their frequency were 0.667 and 0.770,respectively. [Conclusions] We isolate the 3’-UTR sequence of Suhuai pig BMP4 gene and identify a new SNP in the 3’-UTR of BMP4. The polymorphism of 20G>C in the 3’-UTR of BMP4 may be associated with reproductive performance.

参考文献/References:

[1] Bier E,de Robertis E M. BMP gradients:a paradigm for morphogen-mediated developmental patterning[J]. Science,2015,348(6242):aaa5838.
[2] Craft A M,Rockel J S,Nartiss Y,et al. Generation of articular chondrocytes from human pluripotent stem cells[J]. Nature Biotechnology,2015,33(6):638-645.
[3] Yang Y,Adachi K,Sheridan M A,et al. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure[J]. Proceedings of the National Academy of Sciences of the United States of America,2015,112(18):E2337-2346.
[4] Lee J H,Bhang D H,Beede A,et al. Lung stem cell differentiation in mice directed by endothelial cells via a BMP4-NFATc1-thrombospondin-1 axis[J]. Cell,2014,156(3):440-455.
[5] Cao Y,Slaney C Y,Bidwell B N,et al. BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity[J]. Cancer Research,2014,74(18):5091-5102.
[6] Hai Y,Sun M,Niu M,et al. BMP4 promotes human Sertoli cell proliferation via Smad1/5 and ID2/3 pathway and its abnormality is associated with azoospermia[J]. Discovery Medicine,2015,19(105):311-325.
[7] Shimasaki S,Zachow R J,Li D,et al. A functional bone morphogenetic protein system in the ovary[J]. Proceedings of the National Academy of Sciences of the United States of America,1999,96(13):7282-7287.
[8] Al-Samerria S,Al-Ali I,McFarlane J R,et al. The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice[J]. Reproduction,2015,149(5):403-411.
[9] Nilsson E E,Skinner M K. Bone morphogenetic protein-4 acts as an ovarian follicle survival factor and promotes primordial follicle development[J]. Biology of Reproduction,2003,69(4):1265-1272.
[10] Bertoldo M J,Duffard N,Bernard J,et al. Effects of bone morphogenetic protein 4(BMP4)supplementation during culture of the sheep ovarian cortex[J]. Animal Reproduction Science,2014,149(3-4):124-134.
[11] 张海玉,张晓丽,纪淑芳,等. 骨形态发生蛋白4/Smad信号通路在小鼠原始卵泡卵母细胞凋亡中的调控作用[J]. 解剖学报,2014,45(3):375-382. Zhang H Y,Zhang X L,Ji S F,et al. Regulation of bone morphologenetic protein 4/Smad signaling pathway on the apoptosis of mouse primordial follicle oocytes[J]. Acta Anatomica Sinica,2014,45(3):375-382(in Chinese with English abstract).
[12] Chang H M,Cheng J C,Fang L,et al. Recombinant BMP4 and BMP7 downregulate pentraxin 3 in human granulosa cells[J]. The Journal of Clinical Endocrinology & Metabolism,2015,100(3):E365-374.
[13] 姚勇,潘增祥,张久峰,等. 二花脸猪NR5A2基因克隆与卵巢组织转录水平分析[J]. 南京农业大学学报,2013,36(3):133-138. DOI:10.7685/j.issn.1000-2030.2013.03.023 Yao Y,Pan Z X,Zhang J F,et al. Cloning and transcriptional level of NR5A2 gene in ovaries of Erhualian pig[J]. Journal of Nanjing Agricultural University,2013,36(3):133-138(in Chinese with English abstract).
[14] Tu Dg?ul M,Paixão T,Barton N H,et al. Dynamics of transcription factor binding site evolution[J]. PLoS Genetics,2015,11(11):e1005639.
[15] Zhou J,Liu J,Pan Z,et al. The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-β type 1 receptor[J]. Molecular and Cellular Endocrinology,2015,409:103-112.
[16] Zhang Y,Yeh L K,Zhang S,et al. Wnt/β-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development[J]. Development,2015,142(19):3383-3393.
[17] Zhuang W,Ge X,Yang S,et al. Upregulation of lncRNA MEG3 promotes osteogenic differentiation of mesenchymal stem cells from multiple myeloma patients by targeting BMP4 transcription[J]. Stem Cells,2015,33(6):1985-1997.
[18] Borgbo T,Macek M,Chrudimska J,et al. Size matters:associations between the androgen receptor CAG repeat length and the intrafollicular hormone milieu[J]. Molecular and Cellular Endocrinology,2016,419:12-17.
[19] 钟昕. 黄牛BMP2、BMP4及BMPR-IB基因的多态性及其与体尺性状的关联分析[D]. 杨凌:西北农林科技大学,2010. Zhong X. The polymorphism of BMP2,BMP4,BMPR-IB genes and their association with body measurement traits in yellow cattle[D]. Yangling:Northwest A & F University,2010(in Chinese with English abstract).
[20] Liu L,Su Q,Li L,et al. The common variant rs4444235 near BMP4 confers genetic susceptibility of colorectal cancer:an updated meta-analysis based on a comprehensive statistical strategy[J]. PLoS ONE,2014,9(6):e100133.
[21] 张弛. 山羊BMP4基因多态性及其与生产性状关联性的研究[D]. 郑州:河南农业大学,2010. Zhang C. Study on polymorphism of BMP4 gene and relevance with growth traits of goats[D]. Zhengzhou:Henan Agricultural University,2010(in Chinese with English abstract).
[22] 杨华,杨永林,冯静,等. BMP4基因作为中国美利奴羊(军垦型)多胎性能候选基因的研究[J]. 家畜生态学报,2010,31(4):10-13. Yang H,Yang Y L,Feng J,et al. Study of BMP4 as candidate genes for prolificacy in Chinese Merino(Junken type)[J]. Acta Ecologiae Animalis Domasici,2010,31(4):10-13(in Chinese with English abstract).
[23] 储明星,周文然,孙少华,等. 小尾寒羊BMP4基因多态性及其与高繁殖力关系[J]. 农业生物技术学报,2008,16(2):237-241. Chu M X,Zhou W Y,Sun S H,et al. Polymorphism of BMP4 gene and its relationship with prolificacy of Small Tail Han sheep[J]. Journal of Agricultural Biotechnology,2008,16(2):237-241(in Chinese with English abstract).
[24] Sharma R,Ahlawat S,Maitra A,et al. Polymorphism of BMP4 gene in Indian goat breeds differing in prolificacy[J]. Gene,2013,532(1):140-145.

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备注/Memo

备注/Memo:
收稿日期:2016-02-02
基金项目:江苏省农业科技自主创新资金(SCX(12)2097)
作者简介:马百全,硕士研究生。
通信作者:李齐发,教授,主要从事动物遗传育种研究,E-mail:liqifa@njau.edu.cn。
更新日期/Last Update: 1900-01-01