[1]卢毅,李保平,孟玲.氮肥和寄主龄期对斑痣悬茧蜂生长发育特征的影响[J].南京农业大学学报,2016,39(6):960-964.[doi:10.7685/jnau.201601024]
 LU Yi,LI Baoping,MENG Ling.Effects of nitrogen fertilization and host instar on developmental performance of the parasitoid Meteorus pulchricornis Wesmael(Hymenoptera: Braconidae)[J].Journal of Nanjing Agricultural University,2016,39(6):960-964.[doi:10.7685/jnau.201601024]
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氮肥和寄主龄期对斑痣悬茧蜂生长发育特征的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
39卷
期数:
2016年6期
页码:
960-964
栏目:
出版日期:
2016-11-10

文章信息/Info

Title:
Effects of nitrogen fertilization and host instar on developmental performance of the parasitoid Meteorus pulchricornis Wesmael(Hymenoptera: Braconidae)
作者:
卢毅 李保平 孟玲
南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095
Author(s):
LU Yi LI Baoping MENG Ling
College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
关键词:
施肥寄主龄期寄生蜂生活史特征生物防治
Keywords:
fertilizer applicationhost instarparasitoid waspslife history traitsbiological control
分类号:
Q968.1;S476.3
DOI:
10.7685/jnau.201601024
摘要:
[目的]为揭示寄生蜂发育表现是否与土壤施氮量有关,以及该关系是否依赖于寄生时的寄主龄期,对斑痣悬茧蜂(Meteorus pulchricornis)在该因素处理下的生长发育特征进行了研究。[方法]用不同施氮水平(20、50、100、200、300 mg·L-1)的盆栽大豆饲喂斜纹夜蛾(Spodoptera litura)幼虫,分别以2龄和4龄幼虫为寄主供斑痣悬茧蜂寄生,观察子代蜂发育指标。[结果]施氮量、寄主龄期及其互作均显著影响子代蜂存活、历期、成虫体型大小和寿命,但影响的程度和格局不同:施氮量对子代蜂存活和发育历期的影响明显大于寄主龄期,而对子代蜂成虫体型大小和寿命的影响明显小于寄主龄期。子代蜂发育参数与施氮量之间存在非线性关系,但该关系取决于寄生时的寄主龄期:子代蜂存活与施氮量在寄生4龄幼虫时表现出非线性关系,但在寄生2龄幼虫时无显著关系;子代蜂发育历期与施氮量之间存在二次函数曲线关系,但在寄生2龄幼虫时中等水平施氮量的发育历期较长,而寄生4龄幼虫时在较高水平施氮量处理下的发育历期较长;子代蜂成虫体型大小与施氮量之间在寄生2龄幼虫时,表现出正相关直线关系;但寄生4龄幼虫时没有变化;子代成虫寿命在寄生2龄幼虫时与施氮量之间表现出三次函数曲线关系,但在寄生4龄幼虫时没有变化。[结论]土壤施氮可通过寄主龄期大小而影响寄生蜂子代发育表现,而且该影响与施氮量多存在非线性关系。
Abstract:
[Objectives] This study examined the combined effect of nitrogen fertilizer application to soils with host instars at parasitism on developmental performances of the parasitoid Meteorus pulchricornis attacking Spodoptera litura larval hosts feeding on soybean plants on the treated soils. [Methods] Nitrogen fertilizer was assigned to potted soybean seedlings with five-level treatments,the Hoagland’s solution containing nitrogen of 20,50,100,200 and 300 mg·L-1,respectively. The leaves excised from these plants were used to feed S.litura larvae from neonate to either 2nd or 4th instar,which were then exposed to M.pulchricornis for parasitism. Developmental performances of offspring parasitoids were observed. [Results] The N application,host instar at parasitism,and their interactions influenced developmental performances of offspring parasitoids,with variable effects in pattern and magnitude. The N treatment had stronger effects on developmental survival and duration,but weaker effects on offspring adult size and longevity,than the host instar did. Parasitoid developmental parameters changed in a non-linear relationship with the levels of N application,but such relationship varied depending on the host instar at parasitism. Offspring developmental survival increased with N application levels in a parabolic curve from parasitizing the 4th instar larvae,but did not change from parasitizing the 2nd instar larvae. Offspring developmental duration changed as a quadratic function of N application levels,in different shapes between attacking the 2nd and 4th instar larvae. Offspring adult body size increased slightly with N application levels in a linear way from attacking the 2nd,but did not change from parasitizing the 4th instar larvae. Offspring adult longevity changed in as a cubic function of N fertilizer levels from attacking the 2nd instar larvae,but did not change from attacking the 4th instar hosts. [Conclusions] The results of this study suggest that N fertilizer application to soils can influence parasitoid developmental parameters,but such effect occurs often in a non-linear way and vary depending on host stages at oviposition.

参考文献/References:

[1] Schoonhoven L M,van Loon J J A,Dicke M. Insect-Plant Biology[M]. London:Oxford University Press,2005.
[2] Staley J T,Girling R D,Stewart-Jones A,et al. Organic and conventional fertilizer effects on a tritrophic interaction:parasitism,performance and preference of Cotesia vestalis[J]. Journal of Applied Entomology,2011,135:658-665.
[3] Pope T W,Girling R D,Staley J T,et al. Effects of organic and conventional fertilizer treatments on host selection by the aphid parasitoid Diaeretiella rapae[J]. Journal of Applied Entomology,2012,136:445-455.
[4] Garratt M P D,Wright D J,Leather S R. The effects of farming system and fertilizers on pests and natural enemies:a synthesis of current research[J]. Agriculture,Ecosystems and Environment,2011,141:261-270.
[5] Moon D C,Rossi A M,Stilling P. The effects of abiotically induced changes in host plant quality(and morphology)on a salt marsh planthopper and its parasitoid[J]. Ecological Entomology,2000,25:325-331.
[6] Kaneshiro L N,Johnson M W. Tritrophic effects of leaf nitrogen on Liriomyza trifolii and an associated parasitoid Chrysoccharis oschinidis on bean[J]. Biological Control,1996,6:186-192.
[7] 洪晓月,丁锦华. 农业昆虫学[M]. 2版. 北京:中国农业出版社,2007. Hong X Y,Ding J H. Agricultural Entomology[M]. 2nd ed. Beijing:China Agriculture Press,2007(in Chinese).
[8] 何俊华. 浙江蜂类志[M]. 北京:科学出版社,2004. He J H. Hymenoptera Insect Fauna of Zhejing[M]. Beijing:Science Press,2004(in Chinese).
[9] 刘亚慧,李保平. 斑痣悬茧蜂对甜菜夜蛾幼虫龄期的选择及其生长发育的研究[J]. 南京农业大学学报,2006,29(2):66-70. DOI:10.7685/j.issn.1000-2030.2006.02.014. Liu Y H,Li B P. Host-stage selection for Spodoptera exigua larvae and the effect on developmental parameters of solitary endoparasitoid in Meteorus pulchricornis(Hymenoptera:Braconidae)[J]. Journal of Nanjing Agricultural University,2006,29(2):66-70(in Chinese with English abstrct).
[10] Liu Y,Li B. Effects of Helicoverpa armigera(Noctuidae,Lepidoptera)host stages on some developmental parameters of the uniparental endoparasitoid Meteorus pulchricornis(Braconidae,Hymenoptera)[J]. Bulletin of Entomological Research,2008,98:109-114.
[11] 陈雯,李保平,孟玲. 斑痣悬茧蜂对斜纹夜蛾不同虫龄幼虫的选择及后代发育表现[J]. 生态学杂志,2011,30(7):1317-1321. Chen W,Li B P,Meng L. Host selection and offspring developmental performances of Meteorus pulchricornis on Spodoptera litura larval instars[J]. Chinese Journal of Ecology,2011,30(7):1317-1321(in Chinese with English abstract).
[12] 董守坤,刘丽君,张冰,等. 利用15N标记确定氮素营养水平对大豆籽粒氮素构成的影响[J]. 大豆科学,2001,30(1):92-95. Dong S K,Liu L J,Zhang B,et al. Effects of nitrogen nutrition levers on nitrogenous constitution of soybean seeds by15N labeling[J]. Soybean Science,2001,30(1):92-95(in Chinese with English abstract).
[13] 沈晋良,吴益东. 棉龄虫抗药性及其治理[M]. 北京:中国农业出版社,1995:91-94. Shen J L,Wu Y D. Pesticide Resistance and Management of Helicoverpa armigera[M]. Beijing:China Agriculture Press,1995:91-94(in Chinese).
[14] Harrell F E,Jr. Regression Modelling Strategies[M]. 2nd ed. New York:Springer Press,2015.
[15] R Core Team. R:a language and environment for statistical computing[EB/OL]. R Foundation for Statistical Computing,Vienna,Austria.[2014-9-30]. http://www.R-project.org/.
[16] 卢毅,李保平,孟玲. 氮肥对斜纹夜蛾食物利用及生长发育特征的影响[J]. 南京农业大学学报,2014,37(3):72-76. DOI:10.7685/j.issn.1000-2030.2014.03.010. Lu Y,Li B P,Meng L. Effects of nitrogen fertilization on food utilization and developmental parameters in Spodoptera litura Fab.(Lepidoptera:Noctuidae)[J]. Journal of Nanjing Agricultural University,2014,37(3):72-76(in Chinese with English abstract).
[17] Mackauer M,Michaud J P,V lkl W. Host choice by aphidiid parasitoids(Hymenoptera:Aphidiidae):host recognition,host quality,and host value[J]. Canadian Entomologist,1996,128:959-980.
[18] Strand M. Life history variation and developmental constraints in parasitoids[M]//Hochberg M,Ives A R. Parasitoid Population Biologys.New Jersey:Princeton University Press,2000:139-162.
[19] Harvey J A,Strand M R. The developmental strategies of endoparasitoid wasps vary with host feeding ecology[J]. Ecology,2002,83(9):2439-2451.
[20] Beckage N E,Templeton T J,Nielsen B D,et al. Parasitism-induced hemolymph polypeptides in Manduca sexta(L.)larvae parasitized by the braconid wasp Cotesia congregate(Say)[J]. Insect Biochemistry,1987,17:439-455.
[21] Pennacchio F,Vinson S B,Tremblay E. Growth and development of Cardiochiles nigriceps Viereck(Hymenoptera:Braconidae)larvae and their synchronization with some changes of the hemolymph composition of their host,Heliothis virescens(F.)(Lepidoptera:Noctuidae)[J]. Archives of Insect Biochemistry and Physiology,1993,24:65-77.
[22] Pennacchio F,Fanti P,Falabella P,et al. Development and nutrition of the braconid wasp,Aphidius ervi in aposymbiotic host aphids[J]. Archives of Insect Biochemistry and Physiology,1999,40:53-63.
[23] Chen Y,Ruberson J R,Ni X. Influence of host plant nitrogen fertilization on hemolymph protein profiles of herbivore Spodoptera exigua and development of its endoparasitoid Cotesia marginiventris[J]. Biological Control,2014,70:9-16.

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备注/Memo

备注/Memo:
收稿日期:2016-01-13。
基金项目:国家自然科学基金项目(31570389);国家公益性行业(农业)科研专项(201103002)
作者简介:卢毅,硕士研究生。
通信作者:孟玲,教授,主要从事昆虫生态学与害虫生物防治方面的研究,E-mail:ml@njau.edu.cn。
更新日期/Last Update: 1900-01-01