JIANG Guangzhen,ZHANG Yongjing,ZHANG Dingdong,et al.Silicate adsorbent on absorption of Pb2+ and effects of dietary supplement of silicate adsorbent on FFRC strain common carp[J].Journal of Nanjing Agricultural University,2016,39(6):996-1002.[doi:10.7685/jnau.201604039]





Silicate adsorbent on absorption of Pb2+ and effects of dietary supplement of silicate adsorbent on FFRC strain common carp
蒋广震 张永静 张定东 李向飞 刘文斌
南京农业大学动物科技学院/江苏省水产动物营养重点实验室, 江苏 南京 210095
JIANG Guangzhen ZHANG Yongjing ZHANG Dingdong LI Xiangfei LIU Wenbin
College of Animal Science and Technology/Key Laboratory of Aquatic Nutrition of Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China
silicatesPb2+in vivo and in vitro experimentFFRC strain common carpgrowth performancedeposition of Pb2+ on tissues
[目的]通过体内(外)试验,探究硅酸盐吸附剂对饲料铅吸附效果及对福瑞鲤生长、组织铅沉积的影响。[方法]体外试验分别以吸附时间(20、40、60、80、100和120 min)、溶液pH值(2、3、4、5、6、7)和Pb2+起始质量浓度(20、50、100、200 mg·L-1)为变量,以三水合乙酸铅为铅源,考察3种吸附剂(蒙脱石、沸石、凹凸棒土)对其吸附效果。体内试验配制5组试验饲料,分别为:负对照组(基础饲料不含铅)、正对照组(基础饲料添加100 mg·kg-1铅)、蒙脱石组(100 mg·kg-1铅+5 g·kg-1蒙脱石)、沸石组(100 mg·kg-1铅+30 g·kg-1沸石)、棒土组(100 mg·kg-1铅+30 g·kg-1凹凸棒土)。养殖试验选取345尾福瑞鲤,初始体质量约(22.95±0.15)g,随机分为5组,每组3个重复,饲养56 d。[结果]体外试验表明:3种吸附剂的吸附率随吸附时间、溶液pH值的增加均呈升高趋势,随Pb2+起始质量浓度的增加呈先升高后降低的趋势,在吸附时间为40~120 min、pH值为2.0~7.0及Pb2+起始质量浓度为50~200 mg·L-1条件下,蒙脱石的吸附率均高于沸石及凹凸棒土。体内试验结果表明:与对照组相比,3种吸附剂对福瑞鲤的生长性能及组织铅残留均无显著影响(P>0.05);添加吸附剂后,各组织中铅含量均比正对照组低(P>0.05),肝脏组织中铅含量与负对照组相比增加但不显著;蒙脱石组肝脏、鳃中铅含量与负对照组差异不显著。[结论]在本试验条件下,3种硅酸盐对饲料铅均有一定的吸附作用,且蒙脱石的吸附效果优于沸石和凹凸棒土。
[Objectives] This study aimed to evaluate silicate adsorbents on absorption of Pb2+ and the effects of dietary supplement of different silicate adsorbents on FFRC strain common carp. [Methods] In vitro,different time(20,40,60,80,100 and 120 min),pH value(2,3,4,5,6 and 7) and concentration of Pb2+(20,50,100 and 200 mg·L-1) were designed to evaluate the absorption activity of montmorillonite,zeolite and attapulgiteclay. In vivo,five isonitrogenous and isolipidic diets were formulated to contain different adsorbents,such as negative control group(plumbum-free),control group(100 mg·kg-1 Pb2+),montmorillonite group(100 mg·kg-1 Pb2+ and 5 g·kg-1montmorillonite),zeolite group(100 mg·kg-1 Pb2+and 30 g·kg-1 zeolite) and attapulgiteclay group(100 mg·kg-1 Pb2+ and 30 g·kg-1 attapulgiteclay). 345 fish(initial weight:22.95 g) were in 15 aquarium tanks and fed 3 times daily for 56 d. [Results] The results showed that adsorption rate of three silicate adsorbents increased by time,concentration of Pb2+ and pH value,being the highest in 120 min,pH7.0 and concentration of Pb2+ being 50 mg·L-1. No significant difference was found in growth performance of dietary with different silicate adsorbents(P>0.05). Deposition of Pb2+ on liver and gills of montmorillonite group was not significantly different from negative control group(P>0.05). [Conclusions] Based on the above results,the dietary of silicate adsorbents could adsorb the dietary Pb2+ to improve the deposition of Pb2+ on tissues of FFRC strain common carp.


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更新日期/Last Update: 1900-01-01