[1]汤小美,林天元,周珊珊,等.梨树根腐病病原菌的分离与鉴定及有效防控药剂筛选[J].南京农业大学学报,2017,40(1):76-83.[doi:10.7685/jnau.201607002]
 TANG Xiaomei,LIN Tianyuan,ZHOU Shanshan,et al.Pathogen identification of root rot in pear plant and fungicide screening for its efficient control[J].Journal of Nanjing Agricultural University,2017,40(1):76-83.[doi:10.7685/jnau.201607002]
点击复制

梨树根腐病病原菌的分离与鉴定及有效防控药剂筛选()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年1期
页码:
76-83
栏目:
出版日期:
2017-01-08

文章信息/Info

Title:
Pathogen identification of root rot in pear plant and fungicide screening for its efficient control
作者:
汤小美1 林天元1 周珊珊1 李国凤1 刘普1 叶振风1 吴中营2 王东升2 朱立武1
1. 安徽农业大学园艺学院, 安徽 合肥 230036;
2. 河南省农业科学院园艺研究所, 河南 郑州 450002
Author(s):
TANG Xiaomei1 LIN Tianyuan1 ZHOU Shanshan1 LI Guofeng1 LIU Pu1 YE Zhenfeng1 WU Zhongying2 WANG Dongsheng2 ZHU Liwu1
1. College of Horticulture, Anhui Agricultural University, Hefei 230036, China;
2. Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
关键词:
梨树根腐病病原菌鉴定杀菌剂筛选
Keywords:
pear plantroot rotpathogenidentificationfungicidescreening
分类号:
S661.2
DOI:
10.7685/jnau.201607002
摘要:
[目的] 为了明确黄河故道地区造成梨树根腐病的病原菌的种类,研究18种常用杀菌剂对该病原菌的抑制效果。[方法] 通过对梨树根腐病发病根系样品病原菌的分离和单孢纯化,根据代表性菌株形态学观察、rDNA-ITS序列分析和致病性试验,进行病原菌的种类鉴定,对菌丝生长和孢子萌发有效抑制的化学药剂进行筛选,并对部分药剂进行田间防效试验。[结果] 从发病根系样品中分离纯化得到4个菌株,经形态学和rDNA-ITS序列分析,鉴定为同一致病菌;结合致病性试验结果,确定该致病菌是腐皮镰刀菌(Nectria haematococca,无性态Fusarium solani)。化学药剂筛选结果表明:72%霜脲·锰锌、1.8%辛菌胺醋酸盐和80%代森锰锌对腐皮镰刀菌的菌丝生长及孢子萌发均有良好的抑制作用;虽然80%碱式硫酸铜、3%噻霉酮、75%百菌清抑制腐皮镰刀菌菌丝生长效果较差,但对其孢子萌发具有很强的抑制作用。毒力测定与田间试验结果显示:腐皮镰刀菌对4种药剂的敏感性存在差异,95%吡唑醚菌酯和80%代森锰锌对菌丝生长的毒力最强,72%霜脲·锰锌的毒力居中,1.8%辛菌胺醋酸盐的毒力最差;72%霜脲·锰锌、1.8%辛菌胺醋酸盐和80%代森锰锌田间实际防效比95%吡唑醚菌酯好。[结论] 黄河故道地区梨树根腐病由腐皮镰刀菌(Nectria haematococca,无性态Fusarium solani)引起;室内测定和田间试验结果显示,72%霜脲·锰锌、80%代森锰锌或1.8%辛菌胺醋酸盐对梨树根腐病均具有较好的防治效果。
Abstract:
[Objectives] This study was designed to identify the pathogen causing root rot of pear plant in the region of the Old Yellow River Valley,and screen out the efficient chemicals from 18 fungicides commonly used. [Methods] The pathogens were isolated from the tissues of root samples of diseased pear plant and purified by the single spore culture method. The pathogens were identified according to the morphological characters,rDNA-ITS analysis,and pathogenicity tests of strains. The 18 fungicides efficient for suppressing the pathogens were evaluated by calculation of the inhibition rate of mycelium growth and spore germination,and a part of the fungicides were selected for the pathogens control in field trials. [Results] Four strains were isolated and purified from the root samples and were identified as the same pathogen by morphological characteristics and rDNA-ITS analysis. Furthermore,in cooperation with the pathogenicity test,the pathogen was determined as Nectria haematococca,and its anamorph was Fusarium solani. The results of chemical screening showed that 72% cymoxanil+mancozeb,1.8% ametoctradin acetate and 80% mancozeb had good inhibition on both mycelium growth and spore germination of Fusarium solani. Although 80% copper sulfate,3% benziothiazolinone,and 75% chlorothalonil had poor inhibition on mycelium growth,they had very strong inhibition on spore germination of Fusarium solani. The virulences of the four fungicides to Fusarium solani mycelium growth tested in laboratory showed that 95% pyraclostrobin and 80% mancozeb had the best inhibition on mycelium growth of Fusarium solani,followed by 72% cymoxanil+mancozeb,and 1.8% ametoctradin acetate was the worst. After these fungicides had been applied in field,72% cymoxanil+mancozeb,1.8% ametoctradin acetate and 80% mancozeb had better control of the disease than 95% pyraclostrobin. [Conclusions] The root rot of pear plant that occurred in the region of the Old Yellow River Valley was caused by the fungus Nectria haematococca,and their anamorphs were Fusarium solani. The laboratory test and field experiment showed that 72% cymoxanil+mancozeb,80% mancozeb or 1.8% ametoctradin acetate were an effective chemical for the control of root rot in pear production.

参考文献/References:

[1] Ma L J,Geiser D M,Proctor R H,et al. Fusarium pathogenomics[J]. Annual Review of Microbiology,2013,67:399-416.
[2] 肖荣风,刘波,林抗美,等. 斑叶露兜树茎腐病病原鉴定及植物体内菌量测定[J]. 园艺学报,2009,36(2):251-256. Xiao R F,Liu B,Lin K M,et al. Identification and distribution inside plant of pathogen causing stem rot of Pandanus veitchii[J]. Acta Horticulturae Sinica,2009,36(2):251-256(in Chinese with English abstract).
[3] Costa S S,Matos K S,Tessmann D J,et al. Fusarium paranaense sp. nov.,a member of the Fusarium solani species complex causes root rot on soybean in Brazil[J]. Fungal Biology,2016,120(1):51-60.
[4] Zhang J X,Xue A G,Zhang H J,et al. Response of soybean cultivars to root rot caused by Fusarium species[J]. Canadian Journal of Plant Science,2010,90(5):767-776.
[5] Zhang C Q,Liu Y H,Xu B C. First report of Fusarium root rot on Chinese hickory(Carya cathayensis)caused by Fusarium oxysporum in China[J]. Plant Disease,2015,99(9):1284-1286.
[6] Balmas V,Corda P,Marcello A,et al. Fusarium nygamai associated with Fusarium foot rot of rice in Sardinia[J]. Plant Disease,2000,84:801-807.
[7] 石良红,赵兰勇,吴迪,等. 山东牡丹黑斑病的病原菌鉴定与ITS序列分析[J]. 园艺学报,2015,42(3):585-590. Shi L H,Zhao L Y,Wu D,et al. The identification and analysis of ITS sequence on tree peony black spot in Shandong[J]. Acta Horticulturae Sinica,2015,42(3):585-590(in Chinese with English abstract).
[8] 吴良庆,朱立武,衡伟,等. 砀山梨炭疽病病原鉴定及其抑菌药剂筛选[J]. 中国农业科学,2010,43(18):3750-3758. Wu L Q,Zhu L W,Heng W,et al. Identification of Dangshan pear anthracnose pathogen and screening fungicides against it[J]. Scientia Agricultura Sinica,2010,43(18):3750-3758(in Chinese with English abstract).
[9] 周玉霞,程栎菁,张美鑫,等. 我国梨腐烂病病原菌的初步鉴定及序列分析[J]. 果树学报,2013,30(1):140-146. Zhou Y X,Cheng Y J,Zhang M X,et al. Sequence analysis and preliminary identification for the pathogen of pear Valsa canker in China[J]. Journal of Fruit Science,2013,30(1):140-146(in Chinese with English abstract).
[10] 周求根,李诚,蒋军喜,等. 贡梨果实黑斑病病原菌鉴定及室内防治药剂筛选[J]. 中国南方果树,2013,42(5):35-38. Zhou Q G,Li C,Jiang J X,et al. Identification of the pathogen of pear black spot and indoor screening of fungicides[J]. South China Fruit,2013,42(5):35-38(in Chinese with English abstract).
[11] 赵金梅,高贵田,古留杰,等. 中华猕猴桃褐斑病病原鉴定及抑菌药剂筛选[J]. 中国农业科学,2013,46(23):4916-4925. Zhao J M,Gao G T,Gu L J,et al. Identification and pharmaceutical screening of brown spot disease on Actinidia chinensis[J]. Scientia Agricultura Sinica,2013,46(23):4916-4925(in Chinese with English abstract).
[12] 徐成楠,王亚南,胡同乐,等. 蓝莓炭疽病病原菌鉴定及致病性测定[J]. 中国农业科学,2014,47(20):3992-3998. Xu C N,Wang Y N,Hu T L,et al. Identification and pathogenicity of pathogen casuing anthracnose on Vaccinium[J]. Scientia Agricultura Sinica,2014,47(20):3992-3998(in Chinese with English abstract).
[13] 黄欣阳,刘志恒,杨红,等. 辣椒叶斑病的病原菌生物学特性研究[J]. 园艺学报,2013,40(2):275-282. Huang X Y,Liu Z H,Yang H,et al. Biological characteristics of pepper leaf spot pathogen[J]. Acta Horticulturae Sinica,2013,40(2):275-282(in Chinese with English abstract).
[14] 余磊,赵建荣,Rarisara I,等. 蓝莓枯枝病病原菌鉴定[J]. 植物病理学报,2013,43(4):421-425. Yu L,Zhao J R,Rarisara I,et al. Identification of the pathogen causing twigs and stem dieback in blueberry[J]. Acta Phytopathologica Sinica,2013,43(4):421-425(in Chinese with English abstract).
[15] 彭兴民,吴疆翀,郑益兴,等. 云南引种印楝实生种群的表型变异[J]. 植物生态学报,2012,36(6):560-571. Peng X M,Wu J C,Zheng Y X,et al. Phenotypic variation in cultivated populations of Azadirachta indica in Yunnan,China[J]. Chinese Journal of Plant Ecology,2012,36(6):560-571(in Chinese with English abstract).
[16] 陈霞,刘东,张艳菊,等. 黄瓜枯萎病病株镰孢菌的分离与鉴定[J]. 东北农业大学学报,2010,41(7):37-44. Chen X,Liu D,Zhang Y J,et al. Isolation and identification of Fusarium from cucumber wilt plants[J]. Journal of Northeast Agricultural University,2010,41(7):37-44(in Chinese with English abstract).
[17] 管磊,郭贝贝,王晓坤,等. 苯醚甲环唑和氟啶胺的两种制剂包衣种子对花生土传真菌病害的防治效果[J]. 中国农业科学,2015,48(11):2176-2186. Guan L,Guo B B,Wang X K,et al. Control efficacies of two preparations of difenoconazole and fluazinam by seed-coating against peanut soil-borne fungal diseases[J]. Scientia Agricultura Sinica,2015,48(11):2176-2186(in Chinese with English abstract).
[18] 李荣峰,徐秉良,梁巧兰,等. 白菜型冬油菜根腐病病原鉴定及室内毒力测定[J]. 甘肃农业大学学报,2012,47(5):98-104. Li R F,Xu B L,Liang Q L,et al. Identification of root pathogen of winter turnip rapeseed and toxicity measurement[J]. Journal of Gansu Agricultural University,2012,47(5):98-104(in Chinese with English abstract).
[19] 潘龙其,张丽,袁庆华,等. 不同杀菌剂对拟枝孢镰刀菌的毒力测定及田间防效[J]. 中国农业大学学报,2016,21(1):87-96. Pan L Q,Zhang L,Yuan Q H,et al. Toxicity measurement and field control of alfalfa root rot Fusarium sporotrichioide[J]. Journal of China Agricultural University,2016,21(1):87-96(in Chinese with English abstract).
[20] 石爱丽,邢占民,郭玉炜,等. 杀菌剂与杀虫剂配合施用的黄芪根腐病田间防效评价[J]. 河北农业科学,2016,20(1):55-59. Shi A L,Xing Z M,Guo Y W,et al. Field control effect of combination of fungicide and insecticide on root rot disease of Astragalusm embranaceus[J]. Journal of Hebei Agricultural Sciences,2016,20(1):55-59(in Chinese with English abstract).
[21] 涂勇. 果树主要根部病害及其防治方法研究进展[J]. 江苏农业科学,2012,40(10):132-134. Tu Y. The research progress in control methods and main diseases of fruits[J]. Journal of Jiangsu Agricultural Sciences,2012,40(10):132-134(in Chinese with English abstract).
[22] 杨智勇. 观赏果树根部病害的发生规律及综合防治[J]. 陕西农业科学,2005(3):85-88. Yang Z Y. The integrated control and occurred discipline of ornamental fruit trees root disease[J]. Journal of Shaanxi Agricultural Sciences,2005(3):85-88(in Chinese with English abstract).
[23] Whiley A W. 油梨疫霉根腐病的杀菌防治及其对植株水分、产量和环颈病的影响[J]. 云南热作科技,1988,11(4):43-46. Whiley A W. The fungicide control effect of Avocadosp hytophthora root rot and its effect on moisture content,yield and cervical disease[J]. Journal of Yunnan Tropical Crops Science and Technology,1988,11(4):43-46(in Chinese with English abstract).
[24] 曹雪梅,李生兵,张惠玲,等. 甘草根腐病病原菌鉴定[J]. 植物病理学报,2014,44(2):213-216. Cao X M,Li S B,Zhang H L,et al. Identification of the pathogens causing root rot in Glycyrrhiza uralensis Fisch[J]. Acta Phytopathologica Sinica,2014,44(2):213-216(in Chinese with English abstract).
[25] Snyder W C,Hansen H N. The species concept in Fusarium with reference to discolor and other section[J]. American Journal of Botany,1945,32:657-666.
[26] Thangavelu R,Jayanthi A. RFLP analysis of rDNA-ITS regions of native non-pathogentic Fusarium oxysporum isolates and their field evalution for the suppression of Fusarium wilt disease of banana[J]. Australasian Plant Pathology,2009,38:13-21.
[27] Li S,Hartman G L. Molecular detection of Fusarium solani f. sp. glycines in soybean roots and soil[J]. Plant Pathology,2003,52:74-83.

相似文献/References:

[1]张乃文,董彩霞,徐阳春.梨树修剪枝和果实从树体移走的养分研究[J].南京农业大学学报,2013,36(4):37.[doi:10.7685/j.issn.1000-2030.2013.04.007]
 ZHANG Naiwen,DONG Caixia,XU Yangchun.Nutrient amounts removed by the pruning branches and the fruit harvest from the pear tree[J].Journal of Nanjing Agricultural University,2013,36(1):37.[doi:10.7685/j.issn.1000-2030.2013.04.007]
[2]马翠云,封雷,陶书田,等.低温胁迫对梨花器官及授粉受精的影响[J].南京农业大学学报,2014,37(1):53.[doi:10.7685/j.issn.1000-2030.2014.01.010]
 MA Cuiyun,FENG Lei,TAO Shutian,et al.Effects of the low temperature on floral organs and pollination and fertilization of pear[J].Journal of Nanjing Agricultural University,2014,37(1):53.[doi:10.7685/j.issn.1000-2030.2014.01.010]
[3]伍从成,姜海波,赵静文,等.连续5年施用生物有机肥对梨树根系形态及分布的影响[J].南京农业大学学报,2017,40(3):473.[doi:10.7685/jnau.201604056]
 WU Congcheng,JIANG Haibo,ZHAO Jingwen,et al.Effect of continuous application of bio-organic fertilizer for five years on the morphology and distribution of pear roots[J].Journal of Nanjing Agricultural University,2017,40(1):473.[doi:10.7685/jnau.201604056]

备注/Memo

备注/Memo:
收稿日期:2016-07-01。
基金项目:国家现代农业(梨)产业技术体系项目(CARS-29-14)
作者简介:汤小美,硕士研究生。
通信作者:朱立武,教授,研究方向为果树种质资源与遗传育种,E-mail:zhuliwu@ahau.edu.cn。
更新日期/Last Update: 1900-01-01