[1]沈怡斐,鄂垚瑶,阳芳,等.西瓜根系分泌物中氨基酸组分对多黏类芽孢杆菌SQR-21趋化性及根际定殖的影响[J].南京农业大学学报,2017,40(1):101-108.[doi:10.7685/jnau.201603051]
 SHEN Yifei,E Yaoyao,YANG Fang,et al.Effects of amino acids in root exudates of watermelon on the chemotactic reaction and root colonization of Paenibacillus polymyxa SQR-21[J].Journal of Nanjing Agricultural University,2017,40(1):101-108.[doi:10.7685/jnau.201603051]
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西瓜根系分泌物中氨基酸组分对多黏类芽孢杆菌SQR-21趋化性及根际定殖的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年1期
页码:
101-108
栏目:
出版日期:
2017-01-08

文章信息/Info

Title:
Effects of amino acids in root exudates of watermelon on the chemotactic reaction and root colonization of Paenibacillus polymyxa SQR-21
作者:
沈怡斐 鄂垚瑶 阳芳 张楠 黄启为 沈其荣
南京农业大学资源与环境科学学院/国家有机类肥料工程研究中心, 江苏 南京 210095
Author(s):
SHEN Yifei E Yaoyao YANG Fang ZHANG Nan HUANG Qiwei SHEN Qirong
College of Resources and Environmental Sciences/National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China
关键词:
西瓜根系分泌物氨基酸多黏类芽孢杆菌SQR-21趋化定殖
Keywords:
watermelonroot exudatesamino acidsPaenibacillus polymyxa SQR-21chemotaxiscolonization
分类号:
S182
DOI:
10.7685/jnau.201603051
摘要:
[目的] 根系分泌物介导的微生物趋化性是植物促生细菌根际定殖及功能发挥的重要前提,深入理解该过程对指导促生菌的合理施用具有重要意义。[方法] 通过氨基酸分析仪鉴定西瓜根系分泌物中的主要氨基酸组分,并通过滴定分析法(drop assay)和类毛细管法比较促生多黏类芽孢杆菌SQR-21对不同氨基酸组分的趋化性,最终通过原位试验研究外源添加氨基酸对菌株SQR-21在西瓜根际定殖的影响。[结果] 西瓜根系分泌物中包含天冬氨酸、苏氨酸、甘氨酸、半胱氨酸、缬氨酸、甲硫氨酸、异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸、组氨酸、赖氨酸和精氨酸。滴定分析法表明:菌株SQR-21对上述13种氨基酸中除甲硫氨酸和酪氨酸以外的11种氨基酸均存在趋化性,其中对苏氨酸、精氨酸、组氨酸和苯丙氨酸的趋化性较强;类毛细管定量试验进一步表明:菌株SQR-21对不同浓度氨基酸的响应不同,菌株SQR-21对中等浓度的氨基酸(40~80 μmol·L-1)表现出较强的趋化性,其中对精氨酸、组氨酸和苯丙氨酸具有强烈的正趋化作用。综合考虑滴定分析试验和类毛细管试验的结果,设计试验研究原位外源添加精氨酸、组氨酸和苯丙氨酸对菌株SQR-21根际定殖的影响,结果表明60 μmol·L-1的精氨酸、组氨酸和苯丙氨酸均能明显促进菌株SQR-21在西瓜根际的定殖,进一步验证了菌株SQR-21对这3种氨基酸的趋化作用。[结论] 西瓜根系分泌物中的精氨酸、组氨酸和苯丙氨酸可诱导菌株SQR-21对西瓜根系的趋化性并促进其在西瓜根际有效定殖。
Abstract:
[Objectives] Chemotaxis of the plant growth-promoting rhizobacteria(PGPR) is prerequisite for successfully colonizing on the roots and exerting the roles of plant growth promotion and suppression of soil-borne diseases,which is mainly regulated by root exudates. Further understanding of this process is of great significance for guiding the rational application of PGPRs. [Methods] Automatic amino-acid analyzer was applied for identification of the composition of amino acids in watermelon root exudates. Drop assay and capillary tube test were performed to investigate the chemotactic reaction of Paenibacillus polymyxa SQR-21 towards these amino acids. An in situ assay was further performed to study the effects of exogenous amino acids on the colonized population of P. polymyxa SQR-21 recruited by watermelon roots. [Results] Thirteen kinds of amino acids,including aspartic acid,threonine,glycine,cysteine,valine,methionine,isoleucine,leucine,tyrosine,phenylalanine,histidine,lysine and arginine were identified in root exudates of watermelon. Drop assay results indicated that P. polymyxa SQR-21 showed positive chemotactic reactions to 11 kinds of amino acids except methionine and tyrosine,especially to threonine,phenylalanine,arginine,and histidine. Capillary tube test suggested that the chemotaxis responses of strain SQR-21 depend on the concentrations of the amino acids. In detail,strain SQR-21 showed intense chemotactic reaction towards the amino acids within the middle concentration range of 40-80 μmol·L-1,especially arginine,histidine and phenylalanine. On the basis of synthetically considering of drop assay and capillary tube test results,phenylalanine,arginine and histidine were then chosen to study the effects of exogenous amino acids on the colonized population of P. polymyxa SQR-21 recruited by watermelon roots. Results confirmed that adding exogenous arginine,histidine,and phenylalanine of 60 μmol·L-1 in the rhizosphere could promote recruitment to P. polymyxa SQR-21,as revealed by the increased population in the rhizosphere. [Conclusions] This research demonstrated that arginine,histidine and phenylalanine in root exudates of watermelon could induce chemotactic reaction and improve the colonization of P. polymyxa SQR-21 on watermelon roots.

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备注/Memo

备注/Memo:
收稿日期:2016-03-26。
基金项目:国家自然科学基金项目(31272255,31301845);国家公益性行业(农业)科研专项(201503110)
作者简介:沈怡斐,硕士研究生。
通信作者:黄启为,教授,研究方向为新型肥料和有机肥料,E-mail:qwhuang@njau.edu.cn。
更新日期/Last Update: 1900-01-01