WEI Bing,CHEN Wenjuan,ZHU Lin,et al.Immunity enhancement of swine FMDV type-O synthetic peptide vaccine by Th epitope peptides and Poly(I:C)[J].Journal of Nanjing Agricultural University,2017,40(1):163-168.[doi:10.7685/jnau.201601048]





Immunity enhancement of swine FMDV type-O synthetic peptide vaccine by Th epitope peptides and Poly(I:C)
魏冰1 陈文娟1 朱琳1 李建军1 吕传忠2 任东兴2 陈庚2 付旭彬2
1. 天津农学院动物科学与动物医学学院, 天津 300384;
2. 天津瑞普生物技术股份有限公司, 天津 300308
WEI Bing1 CHEN Wenjuan1 ZHU Lin1 LI Jianjun1 LÜ Chuanzhong2 REN Dongxing2 CHEN Geng2 FU Xubin2
1. Animal Science and Animal Medicine College, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Ringpu Biological Technology Co., Ltd., Tianjin 300308, China
foot-and-mouth disease virus(FMDV)Th epitope peptidesPoly(I:C)immunopotentiatorsynthetic peptide vaccine
[目的] 本文旨在研究Th表位肽和聚肌胞苷酸[Poly(I:C)]2种免疫增强剂对猪O型口蹄疫合成肽抗原细胞免疫和体液免疫的增强作用。[方法] 将上述2种免疫增强剂分别与O型口蹄疫病毒(FMDV)合成肽抗原混合乳化,分组免疫Balb/c小鼠,分别在免疫后7、14、21和28 d检测小鼠血清中的VP1抗体、IL-4和IFN-γ水平,以及脾脏中T淋巴细胞转化率及脾脏组织形态变化。[结果] 免疫后14 d Th表位肽疫苗组和Poly(I:C)疫苗组血清VP1抗体水平显著高于普通疫苗组(P<0.05),免疫后28 d Th表位肽疫苗组显著高于Poly(I:C)疫苗组和普通疫苗组(P<0.05)。细胞因子检测结果显示:免疫后14、21 d Th表位肽与Poly(I:C)疫苗组IL-4含量显著高于普通疫苗组(P<0.05),免疫后14 d Th表位肽疫苗组IFN-γ含量明显高于其他组(P<0.05),免疫后21 d Th表位肽与Poly(I:C)疫苗组IFN-γ含量显著高于普通疫苗组(P<0.05)。Th表位肽与Poly(I:C)均可使试验小鼠脾脏白髓区增大,着色加深,动脉周围淋巴鞘胀大,Poly(I:C)疫苗组淋巴细胞增殖系数显著高于其他各试验组(P<0.05)。[结论] Th表位肽和Poly(I:C)对猪O型口蹄疫合成肽抗原均有显著免疫增强作用,而Th表位肽可延长体液免疫持续时间。
[Objectives] This paper was aimed to study the immunity enhancement of swine foot-and-mouth disease virus(FMDV)type O synthetic peptide vaccine by Th epitope peptides and poly inosine-cytidine[Poly(I:C)]. [Methods] Mix these two immunopotentiators with the synthetic peptides of swine FMDV type O and emulsify it separately. Then,inject the Balb/c mice subcutaneously. Explore the change of VP1 antibody,IL-4 and IFN-γ cytokines,T lymphocyte proliferation and morphological observation in spleen at the day of 7,14,21 and 28 after immunization. [Results] The indirect ELISA shows that the levels of VP1 antibody of Th epitope peptides group and Poly(I:C) vaccine group are significantly higher than vaccine group at 14 day after immunization(P<0.05). The levels of VP1 antibody of and Poly(I:C) vaccine group are significantly higher than vaccine group at 14 day after immunization(P<0.05). The Th epitope peptides group expresses significantly higher antibody level than other groups at 28 day. The cytokine analysis shows that Th epitope peptides and Poly(I:C) can significantly increase serum level of IL-4 than the other groups at 14,21 day(P<0.05). Th epitope peptides group expresses higher significantly serum level of IFN-γ than the other groups at 14 day(P<0.05),and the serum levels of IFN-γ of Th epitope peptides group and Poly(I:C) vaccine group are higher than vaccine group. Th epitope peptides and Poly(I:C) can both enlarge the white pulp area of experimental animals’ spleen,deepen the dyeing and swell the peripheral arterial lymphatic sheath. Poly(I:C) vaccine increases significantly lymphocyte proliferation coefficient(P<0.05). [Conclusions] Th epitope peptides and Poly(I:C) can both enhance the immune response of the vaccine,while the Th epitope peptides have a stronger ability to extend the time of humoral immune response.


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 WANG Hao,QIAO Xuwen,CHEN Jin,et al.Concentration and purification of type O foot and mouth disease virus inactivated antigen with Gram-positive bacterial enhancer matrix[J].Journal of Nanjing Agricultural University,2018,41(1):147.[doi:10.7685/jnau.201701100]


更新日期/Last Update: 1900-01-01