[1]顾少涵,潘俏,李子昂,等.黄瓜耐低温突变体材料的筛选与鉴定[J].南京农业大学学报,2017,40(2):219-224.[doi:10.7685/jnau.201606025]
 GU Shaohan,PAN Qiao,LI Ziang,et al.Screening and identification of cold tolerant mutants of cucumber[J].Journal of Nanjing Agricultural University,2017,40(2):219-224.[doi:10.7685/jnau.201606025]
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黄瓜耐低温突变体材料的筛选与鉴定()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年2期
页码:
219-224
栏目:
出版日期:
2017-03-01

文章信息/Info

Title:
Screening and identification of cold tolerant mutants of cucumber
作者:
顾少涵 潘俏 李子昂 陈洁 陈劲枫 娄群峰
南京农业大学作物遗传与种质创新国家重点实验室/园艺学院, 江苏 南京 210095
Author(s):
GU Shaohan PAN Qiao LI Zi’ang CHEN Jie CHEN Jinfeng LOU Qunfeng
State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
黄瓜突变体耐低温性鉴定
Keywords:
cucumbermutantcold toleranceidentification
分类号:
S642.2
DOI:
10.7685/jnau.201606025
摘要:
[目的]本文旨在筛选耐低温的黄瓜突变体,丰富黄瓜耐低温资源材料。[方法]对1 818份‘长春密刺’黄瓜突变体材料进行13℃低温发芽试验,通过比较相对发芽率和胚根生长情况,初步筛选耐低温材料。进一步对筛选出的幼苗进行低温处理(15℃/7℃,昼/夜12 h/12 h,10 d),测定并分析其冷害指数、形态指标、电导率、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。[结果]根据13℃低温发芽试验,将突变体材料分为耐低温型、中间型和冷敏型。其中,耐低温材料共22份,占总材料的1.21%。苗期4项形态指标表明筛选出的耐低温突变体材料在低温胁迫下的整体长势优于对照,并且耐低温材料的冷害指数、电导率、MDA含量和SOD活性也显著低于对照。综合比较后最终鉴定出7份耐低温材料。[结论]通过筛选突变体可以获得耐低温新材料,这些材料的获得对于黄瓜耐低温性状的遗传研究和新品种的培育具有重要的意义和价值。
Abstract:
[Objectives] This article aims to broaden the cold tolerant materials of cucumber. [Methods] The 1 818 ‘Changchunmici’ cucumber mutants materials were preliminarily screened to obtain the candidate cold tolerant lines based on the relative germination rate and radicle growth under 13℃. Further,these lines were treated with 15℃/7℃ in day/night 12 h/12 h for 10 days at seedling stage. Then chilling injury index and morphological indicators were investigated and some physiological index,electrical conductivity,MDA content and SOD activity were analyzed. [Results] According to germination test under 13℃,mutants were divided into tolerant type,middle type and sensitive type. Among them,22 mutants were tolerant type,accounting for 1.21% of the total materials. Under low temperature stress,four morphological indexes of seedling stages indicated that selected cold tolerant mutants grew better than ‘Changchunmici’ and these mutants had a lower value of chilling injury index,electrical conductivity,MDA content and SOD activity than ‘Changchunmici’. Based on the results,7 cucumber lines with tolerance to cold stress were finally identified. [Conclusions] Cold tolerant materials can be obtained from mutants,and these materials will be valuable for the research of chilling tolerance gene and future cucumber breeding.

参考文献/References:

[1] Yu J Q,Zhou Y H,Huang L F,et al. Chill-induced inhibition of photosynthesis:genotypic variation within Cucumis sativus[J]. Plant and Cell Physiology,2002,43(10):1182-1188.
[2] 逯明辉,娄群峰,陈劲枫. 黄瓜的冷害及耐冷性[J]. 植物学通报,2004,21(5):578-586. Lu M H,Lou Q F,Chen J F. A review on chilling injury and cold tolerance in Cucumis sativus L.[J]. Chinese Bulletin of Botany,2004,21(5):578-586(in Chinese with English abstract).
[3] Ruelland E,Vaultier M N,Zachowski A,et al. Cold signalling and cold acclimation in plants[J]. Advances in Botanical Research,2009,49:35-150.
[4] Miller G,Shulaev V,Mittler R. Reactive oxygen signaling and abiotic stress[J]. Physiologia Plantarum,2008,133(3):481-489.
[5] Tewari A K,Tripathy B C. Acclimation of chlorophyll biosynthetic reactions to temperature stress in cucumber(Cucumis sativus L.)[J]. Planta,1999,208(3):431-437.
[6] Mahajan S,Tuteja N. Cold,salinity and drought stresses:an overview[J]. Archives of Biochemistry and Biophysics,2005,444(2):139-158.
[7] Browsea J,Xinb Z. Temperature sensing and cold acclimation[J]. Current Opinion in Plant Biology,2001,4(3):241-246.
[8] 于拴仓,崔鸿文,孟焕文. 黄瓜发芽期耐低温性鉴定方法与指标的研究[J]. 西北农业大学学报,2000,28(3):1-6. Yu S C,Cui H W,Meng H W. Appraisal methods and indexes for low-temperature tolerance in cucumber(Cucumis sativu L.)at seed germination stage[J]. Acta Univ Agric Boreali-Occidentalis,2000,28(3):1-6(in Chinese with English abstract).
[9] 刘雪梅. 黄瓜、辣椒品种早期低温弱光耐受性评价方法研究[D]. 北京:中国农业科学院,2010:9-13. Liu X M.Evaluation method of low temperature and poor light tolerance of cucumber and pepper culitivars at early development stage[D]. Beijing:Chinese Academy of Agricultural Sciences,2010:9-13(in Chinese with English abstract).
[10] 苗永美,宁宇,曹玉杰,等. 黄瓜萌芽期和苗期耐冷性评价[J]. 应用生态学报,2013,24(7):1914-1922. Miao Y M,Ning Y,Cao Y J,et al. Evaluation of cucumber’s chilling tolerance at germination and seedling stages[J]. Chinese Journal of Applied Ecology,2013,24(7):1914-1922(in Chinese with English abstract).
[11] 毛光志,刘彦珍,李建吾. 苗期黄瓜自交系耐低温弱光鉴定研究[J]. 北方园艺,2009(8):85-87. Mao G Z,Liu Y Z,Li J W. Identification of the resistance to low temperature and low light of inbred lines of cucumber at seedling stage[J]. Northern Horticulture,2009(8):85-87(in Chinese with English abstract).
[12] 闫世江. 黄瓜耐低温性的遗传及生理生化特性的研究[D]. 沈阳:沈阳农业大学,2008:23-37. Yan S J. Studies on the inheritance of chilling tolerance and characteristics of physiology-biochemistry in cucumber(Cucumis sativus L.)[D]. Shenyang:Shenyang Agricultural University,2008:23-37(in Chinese with English abstract).
[13] 陈爱葵,韩瑞宏,李东洋,等. 植物叶片相对电导率测定方法比较研究[J]. 广东教育学院学报,2010,30(5):88-91. Chen A K,Han R H,Li D Y,et al. A comparison of two methods for electrical conductivity about plant leaves[J]. Journal of Guangdong Education Institute,2010,30(5):88-91(in Chinese with English abstract).
[14] 王学奎,黄见良. 植物生理生化实验原理与技术[M]. 3版. 北京:高等教育出版社,2015:276-277. Wang X K,Huang J L. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. 3rd ed. Beijing:Higher Education Press,2015:276-277(in Chinese).
[15] 李合生.实验生理生化实验原理和技术[M]. 北京:高等教育出版社,2000:167-169. Li H S. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing:Higher Education Press,2000:167-169(in Chinese).
[16] 王红飞. 黄瓜种质资源低温耐受性的鉴定评价及QTL初步定位研究[D]. 北京:中国农业科学院,2014:12-25. Wang H F. Germplasm identification of tolerance to low temperature and QTL mapping for low temperature adapbility in cucumber[D]. Beijing:Chinese Academy of Agricultural Sciences Dissertation,2014:12-25(in Chinese with English abstract).
[17] 齐晓花,张萍,徐强,等. 黄瓜种子及幼苗期耐冷性鉴定[J]. 中国蔬菜,2011(16):34-38. Qi X H,Zhang P,Xu Q,et al. Evaluation of cold tolerance of cucumber seed and seedling[J]. China Vegetables,2011(16):34-38(in Chinese with English abstract).
[18] 刘建辉,崔鸿文. 电导法鉴定黄瓜抗寒性的研究[J]. 西北农业大学学报,1995,23(4):74-77. Liu J H,Cui H W. Electrical conductivity method for chilling-resistance evaluation in cucumber[J]. Acta Universitatis Agriculturalis Boreali-Occidestalis,1995,23(4):74-77(in Chinese with English abstract).
[19] 毛光志,刘彦珍. 黄瓜杂交组合苗期耐低温弱光鉴定[J]. 湖北农业科学,2009,48(9):2177-2180. Mao G Z,Liu Y Z. Identification of the resistance to low temperature and low light of cross combinations of cucumber in seedling stage[J]. Hubei Agricultural Sciences,2009,48(9):2177-2180(in Chinese with English abstract).
[20] 逯明辉. 黄瓜耐冷生理机制、分子标记及相关基因分离的研究[D]. 南京:南京农业大学,2005:32-38. Lu M H. Studies on the physiological mechanism,molecular markers and related gene isolation of chilling tolerance in cucumber[D]. Nanjing:Nanjing Agricultural University,2005:32-38(in Chinese with English abstract).
[21] 杨国志,齐晓花. 黄瓜种质资源耐低温性鉴定[J]. 浙江农业科学,2015,56(8):1221-1223. Yang G Z,Qi X H. The identification of cold tolerant germplasm resources of cucumber[J]. Journal of Zhejiang Agricultural Sciences,2015,56(8):1221-1223(in Chinese).
[22] Kozik E U,Wehner T C. A single dominant gene Ch for chilling resistance in cucumber seedlings[J]. Journal of the American Society for Horticultural Science,2008,133(2):225-227.
[23] 王昶童. 低温胁迫下嫁接黄瓜耐低温性双向电泳分析[D]. 郑州:郑州大学,2014:18-22. Wang C T. Proteomic analysis of grafted cucumber under cold stress for cold resistance mechanism[D]. Zhengzhou:Zhengzhou University,2014:18-22(in Chinese with English abstract).
[24] 芦露. 黄瓜幼苗对低温胁迫的响应及其耐冷性评价[D]. 哈尔滨:东北农业大学,2014:1-4. Lu L. Evaluation of cold resistance and response of cucumber seedlings to chilling stress[D]. Harbin:Northeast Agricultural University,2014:1-4(in Chinese with English abstract)

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备注/Memo

备注/Memo:
收稿日期:2016-06-19。
基金项目:江苏省农业科技自主创新资金项目[CX(15)1019]
作者简介:顾少涵,本科生。
通信作者:娄群峰,教授,主要从事葫芦科作物遗传育种与种质创新研究,E-mail:qflou@njau.edu.cn。
更新日期/Last Update: 1900-01-01