[1]韩杨会,罗光华,张月亮,等.杀菌剂处理稻苗后对水稻以及取食褐飞虱种群的影响[J].南京农业大学学报,2017,40(2):251-257.[doi:10.7685/jnau.201605017]
 HAN Yanghui,LUO Guanghua,ZHANG Yueliang,et al.Changes of rice seedlings after fungicides treatment and effects of the brown rice planthopper feeding on the treatment rice seedlings[J].Journal of Nanjing Agricultural University,2017,40(2):251-257.[doi:10.7685/jnau.201605017]
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杀菌剂处理稻苗后对水稻以及取食褐飞虱种群的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年2期
页码:
251-257
栏目:
出版日期:
2017-03-01

文章信息/Info

Title:
Changes of rice seedlings after fungicides treatment and effects of the brown rice planthopper feeding on the treatment rice seedlings
作者:
韩杨会12 罗光华2 张月亮2 王利华2 方继朝12
1. 南京农业大学植物保护学院, 江苏 南京 210095;
2. 江苏省农业科学院植物保护研究所, 江苏 南京 210014
Author(s):
HAN Yanghui12 LUO Guanghua2 ZHANG Yueliang2 WANG Lihua2 FANG Jichao12
1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
褐飞虱杀菌剂生理生化物质生命参数
Keywords:
Nilaparvata lugensfungicidephysiological and biochemical substanceslife parameters
分类号:
S435.112+.3;S482.2
DOI:
10.7685/jnau.201605017
摘要:
[目的]探究3种杀菌剂高剂量处理的稻苗对水稻及取食褐飞虱种群的影响。[方法]通过测定杀菌剂处理后稻苗体内草酸、还原糖以及可溶性蛋白的含量,并构建种群生命表探讨经杀菌剂处理的稻苗对取食褐飞虱种群生命参数的影响。[结果]糯稻经3种杀菌剂高剂量处理后,稻苗体内还原糖含量显著降低,碳氮比(C/N)有降低的趋势,草酸和可溶性蛋白的含量无显著变化。粳稻经3种杀菌剂高剂量处理后,C/N有升高的趋势,但草酸、还原糖和可溶性蛋白含量无显著变化。褐飞虱取食杀菌剂高剂量处理的糯稻后,5龄若虫历期、个体总发育历期以及雌成虫寿命显著延长,种群加倍时间、平均世代历期、种群趋势指数以及相对适合度增大;取食杀菌剂高剂量处理的粳稻后,褐飞虱种群趋势指数和种群适合度降低。此外,取食粳稻稻苗的褐飞虱种群趋势指数大于取食糯稻的褐飞虱。[结论]高剂量杀菌剂处理稻苗后能影响稻苗体内相关物质含量的变化,且不同水稻品种所受到的影响存在差异;褐飞虱取食经高剂量杀菌剂处理的稻苗后其部分种群生命参数会发生变化,而且处理的水稻品种不同对取食的褐飞虱影响也不同。
Abstract:
[Objectives] This study aims to determine the effects on rice plant and the population dynamics of the brown planthopper Nilaparvata lugens(BPH). [Methods] The contents of oxalic acid,reducing sugars and soluble proteins in the rice seedlings were determined. And,the population life table of BPH was constructed. [Results] The results showed that the contents of reducing sugar in glutinous rice seedlings treated with 3 fungicides with high doses was significantly lower than the control;the C/N ratio also decreased while had no significantly difference;the contents of oxalic acid and soluble protein also had no significantly changes. On the contrary,the C/N ratio in japonica rice seedlings treated with fungicides increased to some extent. While,the contents of oxalic acid,reducing sugar and soluble protein had no significantly changes. When BPH feeding was applied on the treated glutinous rice seedlings,the development period of the 5 instar nymphs,the full life development duration and the life span of female were all extended,and the population doubling time,the average generation time,the index of population trend and the relative fitness were all increased. When BPH feeding was applied on the treated japonica rice seedlings,the index of population trend and the relative fitness were decreased. It is noteworthy that the index of population trend feeding on japonica rice seedlings was always higher than that feeding on glutinous rice seedlings. [Conclusions] High dose of three fungicides treatment could not only alter the plant physiological and biochemical indexes but also had different effects on the contents of related substances in different varieties of rice seedlings,which could indirectly affect the developmental dynamics of BPH population.

参考文献/References:

[1] Huang H J,Bao Y Y,Lao S H,et al. Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector,the brown planthopper to the rice plant[J]. Scientific Reports,2015,5. DOI:10.1038/srep11413.
[2] Du B,Wei Z,Wang Z Q,et al. Phloem-exudate proteome analysis of response to insect brown plant-hopper in rice[J]. Journal of Plant Physiology,2015,183:13-22.
[3] 包云轩,蒋蓉,谢晓金,等. 近30年气候异常对江苏省褐飞虱灾变性迁入的影响[J]. 生态学报,2014,34(23):7078-7092. Bao Y X,Jiang R,Xie X J,et al. Impact of climate anomalies on catastrophic immigrations of Nilaparvata lugens(St?l)in Jiangsu Province[J]. Acta Ecologica Sinica,2014,34(23):7078-7092(in Chinese with English abstract).
[4] 程家安,祝增荣. 2005年长江流域稻区褐飞虱暴发成灾原因分析[J]. 植物保护,2006,32(4):1-4. Cheng J A,Zhu Z R. Analysis on the key factors causing the outbreak of brown planthopper in Yangtze Area,China in 2005[J]. Plant Protection,2006,32(4):1-4(in Chinese with English abstract).
[5] 陈建明,俞晓平,吕仲贤,等. 稻田常用杀菌剂对褐飞虱若虫的杀伤作用[J]. 中国水稻科学,1998,12(3):155-158. Chen J M,Yu X P,Lü Z X,et al. Insecticidal effects of paddy field fungicides on nymphs of rice brown planthopper[J]. Chinese Journal of Rice Science,1998,12(3):155-158(in Chinese with English abstract).
[6] 陈建明,俞晓平,吕仲贤,等. 除草剂和杀菌剂对褐飞虱及其天敌的影响[J]. 植物保护学报,1999,26(2):162-166. Chen J M,Yu X P,Lü Z X,et al. Effects of herbicides and fungicides on the brown planthopper,Nilaparvata lugens and its predatory enemies[J]. Acta Phytophylacica Sinica,1999,26(2):162-166(in Chinese with English abstract).
[7] Zhang Y X,Zhu Z F,Lu X L,et al. Effects of two pesticides,TZP and JGM,on reproduction of three planthopper species,Nilaparvata lugens St?l,Sogatella furcifera Horvath,and Laodelphax striatella Fallén[J]. Pesticide Biochemistry and Physiology,2014,115:53-57.
[8] Jiang L B,Zhao K F,Wang D J,et al. Effects of different treatment methods of the fungicide jinggangmycin on reproduction and vitellogenin gene(Nlvg)expression in the brown planthopper Nilaparvata lugens St?l(Hemiptera:Delphacidae)[J]. Pesticide Biochemistry and Physiology,2012,102:51-55.
[9] Zhu Z F,Cheng J,Lu X L,et al. Comparisons of topical and spray applications of two pesticides,triazophos and jinggangmycin,on the protein content in the ovaries and fat bodies of the brown planthopper Nilaparvata lugens St?l(Hemiptera:Delphacidae)[J]. Pesticide Biochemistry and Physiology,2014,114:97-101.
[10] 刘井兰,吴进才,袁树忠,等. 经除草剂处理的水稻对褐飞虱体内几种酶及水稻受褐飞虱为害程度的影响[J]. 中国水稻科学,2001,15(4):303-308. Liu J L,Wu J C,Yuan S Z,et al. Effects of the rice treated with herbicides on several enzymes of brown planthopper,and on incidence of rice injury by brown planthopper[J]. Chinese Journal of Rice Science,2001,15(4):303-308(in Chinese with English abstract).
[11] Wu J C,Xu J X,Yuan S Z,et al. Pesticide-induced susceptibility of rice to brown planthopper Nilaparvata lugens[J]. Entomologia Experimentalis et Applicata,2001,100:119-126.
[12] 程耀,赵克非,石朝鹏,等. 农药对水稻体内草酸含量及草酸氧化酶活性的影响[J]. 江苏农业科学,2011,39(5):128-130. Cheng Y,Zhao K F,Shi Z P,et al. Effects of pesticides on oxalate content and oxalate oxidase activity in rice[J]. Jiangsu Agricultural Sciences,2011,39(5):128-130(in Chinese).
[13] 曾宇翔,李西明,马良勇,等. 水稻纹枯病抗性基因定位及抗性资源发掘的研究进展[J]. 中国水稻科学,2010,24(5):544-550. Zeng Y X,Li X M,Ma L Y,et al. Research progress on mapping of gene conferring resistance to sheath blight and exploitation of resistance resources in rice[J]. Chinese Journal of Rice Science,2010,24(5):544-550(in Chinese with English abstract).
[14] 陈再廖,朱浩,莫小平,等. 己唑醇等三唑类药剂防治水稻纹枯病的效果[J]. 浙江农业科学,2010(3):577-579. Chen Z L,Zhu H,Mo X P,et al. Effects of triazoles pesticides(hexaconazole)on controlling rice sheath blight[J]. Journal of Zhejiang Agricultural Sciences,2010(3):577-579(in Chinese).
[15] 傅福全,陈久利,范仰东. 24%噻呋酰胺(满穗)悬浮剂防治水稻纹枯病效果研究[J]. 现代农业科技,2013(1):124-125. Fu F Q,Chen J L,Fan Y D. The research on control effect of 24% thifluzamide(Man Sui)suspension on rice sheath blight[J]. Modern Agricultural Science and Technology,2013(1):124-125(in Chinese).
[16] 陈瑾,吴如健,胡菡青,等. 6种嘧菌酯复配剂对水稻纹枯病的防治效果[J]. 中国农学通报,2013,29(18):162-167. Chen J,Wu R J,Hu H Q,et al. Control effect of 6 types of azoxystrobin mixture on rice sheath blight[J]. Chinese Agricultural Science Bulletin,2013,29(18):162-167(in Chinese with English abstract).
[17] 胡秀荣. 水稻纹枯病菌对井冈霉素的抗性监测及其风险评估[D]. 福州:福建农林大学,2006. Hu X R. The resistance monitoring of Rhizoctonia solani to jinggangmycin and its resistance risk assessment[D]. Fuzhou:Fujian Agriculture and Forestry University,2006(in Chinese with English abstract).
[18] 张继民,钟成义,骆春华. 草酸的分光光度法测定[J]. 安徽机电学院学报,1997,12(4):31-35. Zhang J M,Zhong C Y,Luo C H. Spectrophotometric analysis of oxalic acid[J]. Journal of Anhui Institute of Mechanical and Electrical Engineering,1997,12(4):31-35(in Chinese with English abstract).
[19] 石朝鹏. 农药对转Bt基因水稻生理生化及褐飞虱种群增长的影响[D]. 扬州:扬州大学,2013. Shi Z P. Effects of pesticide applications on the biochemical properties of transgenic Bt rice and the life history parameters of Nilaparvata lugens St?l(Homptera:Delphacidae)[D]. Yangzhou:Yangzhou University,2013(in Chinese with English abstract).
[20] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72:248-254.
[21] 刘泽文,韩召军,王荫长. 褐飞虱抗有机磷品系的交互抗性及适合度研究[J]. 南京农业大学学报,2001,24(4):37-40. DOI:10.7685/j.issn.1000-2030.2001.04.008. Liu Z W,Han Z J,Wang Y C. Cross resistance and relative biological fitness of methamidophos and malathion resistant strains of Nilaparvata lugens(St?l)[J]. Journal of Nanjing Agricultural University,2001,24(4):37-40(in Chinese with English abstract).
[22] 王利华,张月亮,郭慧芳,等. 毒死蜱对灰飞虱抗性和敏感种群的亚致死效应比较[J]. 中国水稻科学,2011,25(5):529-534. Wang L H,Zhang Y L,Guo H F,et al. Comparison of sublethal effects of chlorpyrifos on resistant and susceptible populations of Laodelphax striatellus[J]. Chinese Journal of Rice Science,2011,25(5):529-534(in Chinese with English abstract).
[23] 冉耀贤. 水稻除草剂药害的诊断与补救[J]. 新疆农垦科技,2006(5):34-35. Ran Y X. Diagnosis and salvage of rice herbicide drug induced sufferings symptom[J]. Xinjiang Farm Research of Science and Technology,2006(5):34-35(in Chinese).
[24] 吴进才,许俊峰,冯绪猛,等. 稻田常用农药对水稻3个品种生理生化的影响[J]. 中国农业科学,2003,36(5):536-541. Wu J C,Xu J F,Feng X M,et al. Impacts of pesticides on physiology and biochemistry of rice[J]. Scientia Agricultura Sinica,2003,36(5):536-541(in Chinese with English abstract).
[25] Yoshihara T,Sogawa K,Pathak M D,et al. Oxalic acid as a sucking inhibitor of the brown planthopper in rice(Delphacidae,Homoptera)[J]. Entomologia Experimentalis et Applicata,1980,27:149-155.
[26] Shi Z P,Shang-Gen D U,Yang G Q,et al. Effects of pesticide applications on the biochemical properties of transgenic cry 2A rice and the life history parameters of Nilaparvata lugens St?l(Homptera:Delphacidae)[J]. Journal of Integrative Agriculture,2013,12(9):1606-1613.
[27] 谢道燕,杨振国,田梅金,等. 7种杀菌剂对桑树及家蚕的安全性评价[J]. 中国农学通报,2014,30(22):310-315. Xie D Y,Yang Z G,Tian M J,et al. Safety evaluation of seven fungicides to Morus alba and Bombyx mori[J]. Chinese Agricultural Science Bulletin,2014,30(22):310-315(in Chinese with English abstract).
[28] 黄建华. 不同杀菌剂对柑桔红蜘蛛生物学特性的影响[J]. 华东昆虫学报,1993,2(2):55-59. Huang J H. Effects of fungicides on the biology of the citrus red mite,Panonychus citri(Mcgregor)[J]. Entomological Journal of East China,1993,2(2):55-59(in Chinese with English abstract).
[29] Martinson T,Williams L,English-Loeb G. Compatibility of chemical disease and insect management practices used in New York Vineyards with biological control by Anagrus spp.(Hymenoptera:Mymaridae),parasitoids of Erythroneura leafhoppers[J]. Biological Control,2001,22:227-234.
[30] Carmo E L,Bueno A F,Bueno R C O F. Pesticide selectivity for the insect egg parasitoid Telenomus remus[J]. BioControl,2010,55:455-464.
[31] 程家安,孙祥良. 水稻品种对褐飞虱种群增长的影响[J]. 植物保护学报,1992,19(2):145-151. Cheng J A,Sun X L. The effects of rice varieties on population dynamics of brown planthopper[J]. Acta Phytophylacica Sinica,1992,19(2):145-151(in Chinese with English abstract).
[32] 丁识伯,曾铮,闫凤鸣,等. 褐飞虱在九个水稻品种上的生长发育和生命表参数[J]. 植物保护学报,2012,39(4):334-340. Ding S B,Zeng Z,Yan F M,et al. The development and life table parameters of Nilaparvata lugens(St?l)feeding on nine rice varieties[J]. Acta Phytophylacica Sinica,2012,39(4):334-340(in Chinese with English abstract).

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备注/Memo

备注/Memo:
收稿日期:2016-05-12。
基金项目:江苏省农业科技自主创新资金项目(CX(15)1055);国家自然科学基金项目(31301660);江苏省自然科学基金项目(BK20130724)
作者简介:韩杨会,硕士研究生。
通信作者:方继朝,研究员,主要从事农作物虫害及防治新技术研究,E-mail:fangjc@126.com。
更新日期/Last Update: 1900-01-01