CHENG Rui,ZHANG Huping,CHENG Yinsheng,et al.Bioinformatics analysis of tonoplast monosaccharide transporter(TMT)gene family in fruit trees of Rosaceae[J].Journal of Nanjing Agricultural University,2017,40(4):601-610.[doi:10.7685/jnau.201612028]





Bioinformatics analysis of tonoplast monosaccharide transporter(TMT)gene family in fruit trees of Rosaceae
程瑞 张虎平 程寅胜 王英珍 刘兴 王国明 张绍铃
南京农业大学园艺学院/梨工程技术研究中心, 江苏 南京 210095
CHENG Rui ZHANG Huping CHENG Yinsheng WANG Yingzhen LIU Xing WANG Guoming ZHANG Shaoling
College of Horticulture/Centre of Pear Engineering and Technology Research, Nanjing Agricultural University, Nanjing 210095, China
tonoplast monosaccharide transporter(TMT)Rosaceaesugarexpression
[Objectives]In order to study the functional characterization of tonoplast monosaccharide transporter(TMT)genes in the future,TMT genes were identified in fruit trees of Rosaceae and their structural feature,phylogenetic tree,and expression characteristics in Pyrus bretschneideri were explored. [Methods]Based on the sequenced results of whole genome from Malus domestica,Fragaria×vesca,Prunus mume,Prunus persica,Pyrus bretschneideri,Pyrus communis and Rubus occidentalis,the sequences were analyzed by the method of bioinformation,and the expression characteristic of TMT genes in P. bretschneideri was analysed by RT-qPCR. [Results]The TMT genes number of M. domestica,F. vesca,P. mume,P. persica,P. bretschneideri,P. communis and R. occidentalis was 5,2,4,4,6,4,2,respectively. All of the TMT genes were highly conservative,and the amino acid sequences of all TMT genes were about 700,which contained 11 putative transmembrane α-helices,the theoretical isoelectric point(pI)from 4.79 to 5.93,the grand average of hydropathicity between 0.312 and 0.438,and the protein sequences were located in the vacuole membrane based on the subcellular localization prediction. Phylogenetic tree analysis showed that TMT genes could be classified into two groups. GroupⅠcontained main functional genes of TMT genes family. Evolutionary analysis showed that there were two whole genome duplication events of TMT gene family during the evolution process,and the selective pressure was main factors. The expression analysis in P. bretschneideri showed that the PbTMT1,PbTMT2 and PbTMT3 had higher expression in mature leaves,the expression of PbTMT4 increased with the development of fruit,and PbTMT6 had higher expression in mature leaf but declined sharply during the late stage of fruit development. [Conclusions]TMT gene family in fruit trees of Rosaceae contained a highly conserved domain,and the TMT genes localized in the vacuole membrane not only involved in fruit sugar accumulation but also involved in plant development and stress resistance.


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更新日期/Last Update: 2017-07-07