[1]沈子杰,范德佳,何俊,等.籼稻品种‘IR13427-45-2-1-2-2-2’抗白背飞虱QTL定位[J].南京农业大学学报,2017,40(6):957-962.[doi:10.7685/jnau.201704027]
 SHEN Zijie,FAN Dejia,HE Jun,et al.Mapping of the quantitative trait locus conferring white-backed planthopper resistance in indica cultivar ‘IR13427-45-2-1-2-2-2’[J].Journal of Nanjing Agricultural University,2017,40(6):957-962.[doi:10.7685/jnau.201704027]
点击复制

籼稻品种‘IR13427-45-2-1-2-2-2’抗白背飞虱QTL定位()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年6期
页码:
957-962
栏目:
出版日期:
2017-11-10

文章信息/Info

Title:
Mapping of the quantitative trait locus conferring white-backed planthopper resistance in indica cultivar ‘IR13427-45-2-1-2-2-2’
作者:
沈子杰1 范德佳1 何俊1 黄捷1 江玲1 程遐年1 刘裕强1 万建民12
1. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏 南京 210095;
2. 中国农业科学院作物科学研究所, 北京 100081
Author(s):
SHEN Zijie1 FAN Dejia1 HE Jun1 HUANG Jie1 JIANG Ling1 CHENG Xianian1 LIU Yuqiang1 WAN Jianmin12
1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
水稻白背飞虱抗虫基因定位
Keywords:
Oryza sativa L.white-backed plant hopperinsect resistancequantitative trait loci
分类号:
S511.032
DOI:
10.7685/jnau.201704027
摘要:
[目的] 白背飞虱及其传播的南方黑条矮缩病危害日益加剧,严重危及水稻生产。本研究旨在发掘水稻抗白背飞虱基因,为抗白背飞虱品种培育提供有用的基因资源。[方法] 利用高抗白背飞虱水稻品种‘IR13427-45-2-1-2-2-2’(简称‘IR13427’)与感虫品种‘宁粳3号’构建1个包含92个家系的F2:3群体,进行白背飞虱抗性鉴定,选取极端个体进行连锁分析,构建有连锁迹象的染色体连锁图谱,运用MAPMAKER/EXP Version 3.0软件进行QTL (quantitative trait locus)检测。[结果] 利用10个极感和12个极抗个体,在第5、6和9染色体上发现连锁迹象,构建了这3条染色体的连锁图谱,进行QTL检测,结果在第5和第6染色体检测到2个QTL,分别命名为qWBPH5qWBPH6,贡献率分别为11.90%和18.30%,LOD值为2.42和3.28。[结论] 籼稻品种‘IR13427’对白背飞虱的抗性由数量性状位点控制,本研究检测到2个抗白背飞虱QTL,为水稻抗白背飞虱基因的克隆及品种培育奠定基础。
Abstract:
[Objectives] The increasing damage caused by the white-backed plant hopper (WBPH)and its transmitting southern rice black-streaked dwarf virus (SRBSDV)seriously threatens the rice (Oryza sativa L.)production. The aim of present study is to identify WBPH-resistance genes for developing resistance cultivars.[Methods] We constructed an F2:3 population containing 92 lines derived from a cross between highly resistant indica variety, ‘IR13427-45-2-1-2-2-2’, and susceptible japonica variety, ‘Ningjing 3’, and then evaluated it for WBPH resistance. The candidate chromosomes harboring WBPH resistance QTL (quantitative trait locus)were screened by extreme resistant and susceptible lines, then the molecular linkage map was constructed to conduct QTL analysis with MAPMAKER/EXP Version 3.0.[Results] Chromosome 5, 6 and 9 were identified as candidate chromosomes with WBPH resistance QTL using 10 high susceptible and 12 resistant lines, respectively. Then the molecular linkage maps of these three chromosomes were constructed. Two QTL, qWBPH5 and qWBPH6, conferring WBPH resistance were detected. qWBPH5 was located on chromosome 5 with a LOD score of 2.42, explaining 11.90% of the phenotypic variance. qWBPH6 was mapped on chromosome 6 with a LOD score of 3.28, explaining 18.30% of the phenotypic variance.[Conclusions] The WBPH resistance of the indica variety, ‘IR13427-45-2-1-2-2-2’, may be contributed by quantitative trait loci. The newly identified two QTL conferring WBPH resistance in this study provides the foundation for cloning of rice WBPH-resistance genes and developing resistant cultivars to reduce the damage caused by WBPH and SRBSDV.

参考文献/References:

[1] 徐安隆,陈曙,贺文爱,等. 水稻抗褐飞虱与白背飞虱研究进展[J]. 南方农业学报,2015,46(9):1628-1635. Xu A L,Chen S,He W A,et al. Progress on rice resistance to brown planthopper and white-backed planthopper[J]. Journal of Southern Agriculture,2015,46(9):1628-1635(in Chinese with English abstract).
[2] 邓伟,胡兰香,陈红萍,等. 水稻抗白背飞虱研究进展[J]. 江西农业学报,2012,24(1):91-97,105. Deng W,Hu L X,Chen H P,et al. Research progress in resistance of rice to Sogatella furcifera[J]. Acta Agriculturae Jiangxi,2012,24(1):91-97,105(in Chinese with English abstract).
[3] Yamasaki M,Yoshimura A,Yasui H. Genetic basis of ovicidal response to white-backed planthopper(Sogatella furcifera Horváth)in rice(Oryza sativa L.)[J]. Molecular Breeding,2003,12(2):133-143.
[4] 周国辉,张曙光,邹寿发,等. 水稻新病害南方水稻黑条矮缩病发生特点及危害趋势分析[J]. 植物保护,2010,36(2):144-146. Zhou G H,Zhang S G,Zou S F,et al. Occurrence and damage analysis of a new rice dwarf disease caused by Southern rice black-streaked dwarf virus[J]. Plant Protection,2010,36(2):144-146(in Chinese with English abstract).
[5] Zhang H M,Yang J,Chen J P,et al. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus[J]. Archives of Virology,2008,153(10):1893-1898.
[6] 郭荣,周国辉,张曙光. 水稻南方黑条矮缩病发生规律及防控对策初探[J]. 中国植保导刊,2010,30(8):17-20. Guo R,Zhou G H,Zhang S G. Study on the occurrence and control of Southern rice black-streaked dwarf virus disease[J]. China Plant Protection,2010,30(8):17-20(in Chinese).
[7] Sidhu G S,Khush G S,Medrano F G. A dominant gene in rice for resistance to white-backed planthopper and its relationship to other plant characteristics[J]. Euphytica,1979,28(2):227-232.
[8] 黄得润,龚俊义. 水稻抗褐飞虱与白背飞虱基因定位研究进展[J]. 科技通报,2009,25(4):412-418. Huang D R,Gong J Y. Advances in molecular mapping of resistance genes to brown planthopper and whitebacked planthopper in rice[J]. Bulletin of Science and Technology,2009,25(4):412-418.
[9] 刘志岩,刘光杰,寒川一成,等. 水稻抗白背飞虱基因Wbph2的初步定位[J]. 中国水稻科学,2002,16(4):311-314. Liu Z Y,Liu G J,Han C Y C,et al. Mapping the gene Wbph2 in ARC10239 resistant to the whitebacked planthopper,Sogatella furcifera in rice[J]. Chinese Journal of Rice Science,2002,16(4):311-314(in Chinese with English abstract).
[10] Wu C F,Khush G S. A new dominant gene for resistance to whitebacked planthopper in rice[J]. Crop Science,1985,25(3):505-509.
[11] 李西明,闵绍楷,熊振民,等. 水稻品种对白背飞虱的抗源筛选及其抗性遗传分析[J]. 遗传学报,1987,14(6):413-418. Li X M,Min S K,Xiong Z M,et al. Screening of rice germplasms resistant to white-backed planthopper and their genetic analysis[J]. Acta Genetica Sinica,1987,14(6):413-418(in Chinese).
[12] Tan G X,Weng Q M,Ren X,et al. Two white-backed planthopper resistance genes in rice share the same loci with those for brown planthopper resistance[J]. Heredity,2004,92(3):212-217.
[13] Buna W,Zhen H,Li H S,et al. Mapping of two new brown planthopper resistance genes from wild rice[J]. Chinese Science Bulletin,2001,46(13):1092-1095.
[14] Huang Z,He G,Shu L,et al. Identification and mapping of two brown planthopper resistance genes in rice[J]. Theoretical and Applied Genetics,2001,102(6/7):929-934.
[15] Yang Y L,Xu J,Leng Y J,et al. Quantitative trait loci identification,fine mapping and gene expression profiling for ovicidal response to whitebacked planthopper(Sogatella furcifera Horvath)in rice(Oryza sativa L.)[J]. BMC Plant Biology,2014,14(1):1-16.
[16] 朱麟,古德祥,张古忍. 水稻抗性品种对褐飞虱和白背飞虱羧酸酯酶活力影响的比较研究[J]. 中山大学学报(自然科学版),2003,39(增刊2):67-71. Zhu L,Gu D X,Zhang G R. Comparative study on the effects of rice resistance on the activity of acetic acid and vinegar in brown planthopper and white-backed planthopper[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni,2003,39(Suppl 2):67-71(in Chinese with English abstract).
[17] 朱麟,张古忍,古德祥. 抗性水稻品种对褐飞虱和白背飞虱种群参数的影响[J]. 昆虫知识,2002,39(4):265-268. Zhu L,Zhang G R,Gu D X. Effects of resistance rice varieties on population parameters of brown planthopper and white-backed planthopper[J]. Entomological Knowledge,2002,39(4):265-268(in Chinese with English abstract).
[18] 张富铁,庄延,杨之帆,等. 药用野生稻应答稻飞虱取食过程中基因表达的研究[J]. 武汉植物学研究,2005,23(1):1-6. Zhang F T,Zhuang Y,Yang Z F,et al. Wild rice gene expression in response to brown planthopper(Nilaparvata lugens Stål)feeding and white-backed planthopper(Sogatella furcifera Horváth)feeding[J]. Journal of Wuhan Botanical Research,2005,23(1):1-6(in Chinese with English abstract).
[19] 黄凤宽,黄所生,吴碧球,等. 抗褐飞虱兼抗白背飞虱水稻种质资源发掘[J]. 植物保护,2012,38(4):152-155. Huang F K,Huang S S,Wu B Q,et al. Screening of rice germplasms resistant to brown planthopper(BPH)and white-backed planthopper(WBPH)[J]. Plant Protection,2012,38(4):152-155(in Chinese with English abstract).
[20] 俞晓平,巫国瑞,胡萃. 水稻品种对白背飞虱的抗性及其与稻株营养成分的关系[J]. 中国水稻科学,1989,3(2):56-61. Yu X P,Wu G R,Hu C. The rice varietal resistance to white-backed planthopper(Sogatella furcifera)and the relationship between the nutrients in rice plants and varietal resistance[J]. Chinese Journal of Rice Science,1989,3(2):56-61(in Chinese with English abstract).
[21] 刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J]. 遗传,2003,25(3):317-321. Liu R H,Meng J L. MapDraw:amicrosoft excel macro for drawing genetic linkage maps based on given genetic linkage data[J]. Genetics,2003,25(3):317-321(in Chinese with English abstract).
[22] 刘光杰,付志红,沈君辉,等. 水稻品种对稻飞虱抗性鉴定方法的比较研究[J]. 中国水稻科学,2002,16(1):52-56. Liu G J,Fu Z H,Shen J H,et al. Comparative study on evaluation methods for resistance to rice planthoppers(Homoptera:Delphacidae)in rice[J]. Chinese Journal of Rice Science,2002,16(1):52-56(in Chinese with English abstract).
[23] Tuyen L Q,Liu Y,Jiang L,et al. Identification of quantitative trait loci associated with small brown planthopper(Laodelphax striatellus Fallen)resistance in rice(Oryza sativa L.)[J]. Hereditas,2012,149(1):16-23.
[24] Sun L,Liu Y,Jiang L,et al. Identification of quantitative trait loci associated with resistance to brown planthopper in the indica rice cultivar Col.5 Thailand[J]. Hereditas,2007,144(2):48-52.
[25] Kawaguchi M,Murata K,Ishii T,et al. Assignment of a brown planthopper(Nilaparvata lugens Stål)resistance gene bph4 to the rice chromosome 6[J]. Breeding Science,2001,51(1):13-18.
[26] Wang Y,Cao L,Zhang Y,et al. Map-based cloning and characterization of BPH29,a B3 domain-containing recessive gene conferring brown planthopper resistance in rice[J]. Journal of Experimental Botany,2015,66(19):6035-6045.
[27] Ren J,Gao F,Wu X,et al. Bph32,a novel gene encoding an unknown SCR domain-containing protein,confers resistance against the brown planthopper in rice[J]. Scientific Reports,2016,6:37645.

相似文献/References:

[1]张辰明,徐烨红,赵海娟,等.不同氮形态对水稻苗期氮素吸收和根系生长的影响[J].南京农业大学学报,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(6):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
[2]郝文雅,沈其荣,冉炜,等.西瓜和水稻根系分泌物中糖和氨基酸对西瓜枯萎病病原菌生长的影响[J].南京农业大学学报,2011,34(3):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
 HAO Wen-ya,SHEN Qi-rong,RAN Wei,et al.The effects of sugars and amino acids in watermelon and rice root exudates on the growth of Fusarium oxysporum f.sp. niveum[J].Journal of Nanjing Agricultural University,2011,34(6):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
[3]徐小飒,刘喜,赵志刚,等.培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测[J].南京农业大学学报,2011,34(1):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
 XU Xiao-sa,LIU Xi,ZHAO Zhi-gang,et al.Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight[J].Journal of Nanjing Agricultural University,2011,34(6):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
[4]魏广彬,徐海港,丁艳峰,等.水稻设计栽培系统的研制与实现[J].南京农业大学学报,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
 WEI Guang-bin,XU Hai-gang,DING Yan-feng,et al.Development and realization of the rice design cultivation system[J].Journal of Nanjing Agricultural University,2011,34(6):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
[5]李刚华,王惠芝,王绍华,等.穗肥对水稻穗分化期碳氮代谢及颖花数的影响[J].南京农业大学学报,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
 LI Gang-hua,WANG Hui-zhi,WANG Shao-hua,et al.Effect of nitrogen applied at rice panicle initiation stage on carbon and nitrogen metabolism and spikelets per panicle[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
[6]王碧茜,范晓荣,徐国华,等.不同氮效率水稻品种旗叶的衰老特征[J].南京农业大学学报,2010,33(2):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
 WANG Bi-qian,FAN Xiao-rong,XU Guo-hua,et al.Characteristics of flag leaf senescence among three rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(6):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
[7]赵成国,徐海港,李刚华,等.超高产单季粳稻抽穗期群体构成研究[J].南京农业大学学报,2011,34(2):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
 ZHAO Cheng-guo,XU Hai-gang,LI Gang-hua,et al.Studies on population composition of super-high-yielding single-cropping japonica rice in heading stage[J].Journal of Nanjing Agricultural University,2011,34(6):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
[8]陈志德,仲维功,王军,等.水稻苗期Cd2+胁迫的QTL定位研究[J].南京农业大学学报,2010,33(3):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
 CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
[9]叶利庭,樊剑波,徐晔红,等.不同氮效率水稻的生长特性[J].南京农业大学学报,2010,33(3):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
 YE Li-ting,FAN Jian-bo,XU Ye-hong,et al.Characteristics of growth in rice genotypes with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(6):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
[10]晋玉宽,杨世湖,余丽,等.不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系[J].南京农业大学学报,2010,33(4):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(6):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]

备注/Memo

备注/Memo:
收稿日期:2017-04-17。
基金项目:国家重点研发计划项目(2016YFD0100603-9);国家自然科学基金项目(31522039,31471470);江苏省自然科学基金项目(BK20150026)
作者简介:沈子杰,硕士研究生。
通信作者:刘裕强,教授,博导,研究方向为水稻遗传育种,E-mail:yql@njau.edu.cn。
更新日期/Last Update: 1900-01-01