[1]张曼,戴蓉,张顺凯,等.H2O2浸种对油菜种子低温萌发的缓解效应[J].南京农业大学学报,2017,40(6):963-970.[doi:10.7685/jnau.201702029]
 ZHANG Man,DAI Rong,ZHANG Shunkai,et al.Alleviation effects of seed soaking with H2O2on seed germination in rape under low temperature stress[J].Journal of Nanjing Agricultural University,2017,40(6):963-970.[doi:10.7685/jnau.201702029]
点击复制

H2O2浸种对油菜种子低温萌发的缓解效应()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年6期
页码:
963-970
栏目:
出版日期:
2017-11-10

文章信息/Info

Title:
Alleviation effects of seed soaking with H2O2on seed germination in rape under low temperature stress
作者:
张曼 戴蓉 张顺凯 江海东
南京农业大学农业部南方作物生理生态重点开放实验室/江苏省现代作物生产协同创新中心, 江苏 南京 210095
Author(s):
ZHANG Man DAI Rong ZHANG Shunkai JIANG Haidong
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China
关键词:
甘蓝型油菜浸种过氧化氢低温萌发
Keywords:
Brassica napusseed soakingH2O2low temperaturegermination
分类号:
S565.4
DOI:
10.7685/jnau.201702029
摘要:
[目的] 低温会延长晚直播油菜萌发时间,缩短冬前生长期,降低油菜越冬期抗寒性,因此采取有效措施促进油菜低温萌发具有重要意义。[方法] 以甘蓝型油菜品种‘中双11号’(敏感型)和‘南农油4号’(耐寒型)为材料,采用种子萌发试验,设置(22±1)℃正常对照(CK)、(10±1)℃低温处理(LT)和(10±1)℃过氧化氢(H2O2)浸种3个处理,研究了H2O2浸种处理对油菜种子萌发过程中低温胁迫的缓解效应。[结果] 低温胁迫显著降低2个品种油菜种子发芽势、发芽率及发芽指数,延长平均发芽时间(P<0.05)。H2O2浸种处理可提高种子内的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及抗坏血酸(AsA)和谷胱甘肽(GSH)含量,有效抑制活性氧(ROS)产生,降低细胞膜质过氧化程度,并且促进抗寒基因Cu/Zn-SODBnICE1表达量的增加。0.05% H2O2浸种处理后,2个品种种子的发芽势、发芽率以及发芽指数显著增加,平均发芽时间显著减少,表现出较高的萌发能力。[结论] H2O2浸种处理可促进油菜种子抗寒基因的表达,提高油菜种子低温萌发过程中的抗氧化性能,增强油菜种子耐低温萌发能力。
Abstract:
[Objectives] Low temperature can delay seed germination and shorten growing days before winter so as to decrease the cold tolerance in winter. It’s of great effects to take suitable practice to promote seed germination in rape.[Methods] Germination experiments with ‘Zhongshuang 11’ (cold-sensitive)and ‘Nannongyou 4’ (cold-tolerant)were conducted to study the effects of seed soaking with H2O2 on seed germination under low temperature stress. In this experiment, three treatments were designed:CK, (22±1)℃;DT, (10±1)℃;H2O2, (10±1)℃ with H2O2 treatment.[Results] Under low temperature stress, the germination potential, germination rate and germination index of two rape varieties decreased significantly, and the mean germination time delayed (P<0.05). H2O2 pre-treatment significantly promoted the cold resistance gene expression of both Cu/Zn-SOD and BnICE1, and increased the activities of superoxide dismutase (SOD), peroxidase (POD)and catalase (CAT), and the contents of ascorbic acid (AsA)and glutathione (GSH), so as to suppress reactive oxygen species (ROS)generation and cell membrane lipid peroxidation. The seed germination potential, germination rate and index of two varieties increased significantly, while the mean germination time reduced significantly under low temperature stress with 0.05% H2O2, suggesting that the seeds with H2O2 soaking had a higher germination ability.[Conclusions] Soaking with H2O2 could enhance the germination ability under low temperature of rape seeds by increasing antioxidant ability and promoting expression of cold tolerant genes.

参考文献/References:

[1] Rajjou L,Duval M,Gallardo K,et al. Seed germination and vigor[J]. Annual Review of Plant Biology,2012,63:507-533.
[2] 宋兆伟,郝丽珍,黄振英,等. 光照和温度对沙芥和斧翅沙芥植物种子萌发的影响[J]. 生态学报,2010,30(10):2562-2568. Song Z W,Hao L Z,Huang Z Y,et al. Effects of light and temperature on the germination of Pugionium cornutum(L.)Gaertn. and Pugionium dolabratum Maxim. seeds[J]. Acta Ecologica Sinica,2010,30(10):2562-2568(in Chinese with English abstract).
[3] 盛海燕,葛滢,常杰,等. 环境因素对伞形科两种植物种子萌发的影响[J]. 生态学报,2004,24(2):221-226. Sheng H Y,Ge Y,Chang J,et al. Influence of environmental factors on seed germination of two species in Umbellaceae[J]. Acta Ecologica Sinica,2004,24(2):221-226(in Chinese with English abstract).
[4] Wang W Q,Møller I M,Song S Q. Proteomic analysis of embryonic axis of Pisum sativum seeds during germination and identification of proteins associated with loss of desiccation tolerance[J]. Journal of Proteomics,2012,77:68-86.
[5] Jiang J L,Su M,Wang LY,et al. Exogenous hydrogen peroxide reversibly inhibits root gravitropism and induces horizontal curvature of primary root during grass pea germination[J]. Plant Physiology and Biochemistry,2012,53:84-93.
[6] Su L Q,Lan Q Y,Pritchard H W,et al. Reactive oxygen species induced by cold stratification promote germination of Hedysarum scoparium seeds[J]. Plant Physiology and Biochemistry,2016,109:406-415.
[7] 张立军,宋广周,白霜,等. H2O2对不同大豆品种低温萌发及主要抗氧化酶活性的影响[J]. 大豆科学,2008,27(1):97-100,105. Zhang L J,Song G Z,Bai S,et al. Effect of exogenous H2O2 on germination rate and activities of main antioxidase in soybean(Glycine max L.)seedlings under low temperature[J]. Soybean Science,2008,27(1):97-100,105(in Chinese with English abstract).
[8] 蒋景龙. 外源H2O2对低温胁迫下柑橘叶片抗寒性的影响[J]. 西北植物学报,2016,36(3):499-505. Jiang J L. Effect of exogenous H2O2 treatment on cold resistance of Citrus reticulata leaves under low temperature stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2016,36(3):499-505(in Chinese with English abstract).
[9] 韩娜,冯汉青,李燕鸿,等. 外源H2O2对水稻幼苗抗寒性的调节作用[J]. 西北植物学报,2009,29(6):1214-1219. Han N,Feng H Q,Li Y H,et al. Regulation of exogenous H2O2 on rice seedlings chilling tolerance[J]. Acta Botanica Boreali-Occidentalia Sinica,2009,29(6):1214-1219(in Chinese with English abstract).
[10] Yu C W,Murphy T M,Sung W W,et al. H2O2treatment induces glutathione accumulation and chilling tolerance in mung bean[J]. Functional Plant Biology,2002,29:1081-1087.
[11] 万林,张曼,钟飞燕,等. H2O2浸种处理对晚直播油菜苗期耐寒性的影响[J]. 中国油料作物学报,2015,37(6):811-819. Wan L,Zhang M,Zhong F Y,et al. Effects of seed soaking with hydrogen peroxide on seedling cold tolerance of late direct-sowing rape[J]. Chinese Journal of Oil Crop Sciences,2015,37(6):811-819(in Chinese with English abstract).
[12] Wang Y R,Yu L,Nan Z B,et al. Vigor tests used to rank seed lot quality and predict field emergence in four forage species[J]. Crop Science,2004,44:535-541.
[13] 刘克彪,姜生秀. 干旱和钠盐胁迫对罗布麻种子萌发的影响[J]. 草业学报,2016,25(5):214-221. Liu K B,Jiang S X. Responses of Apoceynum venetum seed germination to drought and salt stress[J]. Acta Prataculturae Sinica,2016,25(5):214-221(in Chinese with English abstract).
[14] Hodges D M,de Long J M,Forney C F,et al. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J]. Planta,1999,207:604-611.
[15] Tan W,Liu J,Dai T,et al. Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging[J]. Photosynthetica,2008,46(1):21-27.
[16] Chance B,Maehly A C. Assay of catalases and peroxidases[J]. Methods in Enzymology,1955,2(55):764-775.
[17] Khanna-Chopra R,Selote D S. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than-susceptible wheat cultivar under field conditions[J]. Environmental and Experimental Botany,2007,60:276-283.
[18] Paradiso A,Berardino R,de Pinto M C,et al. Increase in ascorbate-glutathione metabolism as local and precocious systemic responses induced by cadmium in durum wheat plants[J]. Plant Cell Physiol,2008,49(3):362-374.
[19] 丁勇,刘英,杨鸯鸯,等. 油菜种子高质量总RNA提取的一种有效方法[J]. 华中农业大学学报,2006,25(5):465-468. Ding Y,Liu Y,Yang Y Y,et al. An efficient method for extraction of high quality RNA from rapeseeds[J]. Journal of Huazhong Agricultural University,2006,25(5):465-468(in Chinese with English abstract).
[20] 孙海波,王智慧,刘学群,等. 一种适用于油菜、大豆、花生、芝麻四种油料作物种子的RNA提取方法[J]. 中国油料作物学报,2012,34(4):353-358. Sun H B,Wang Z H,Liu X Q,et al. Modified RNA extraction method for seeds of rapeseed,soybean,peanut and sesame[J]. Chinese Journal of Oil Crop Sciences,2012,34(4):353-358(in Chinese with English abstract).
[21] 马骊,孙万仓,刘自刚,等. 低温胁迫下白菜型与甘蓝型冬油菜抗寒基因表达差异[J]. 中国油料作物学报,2016,38(2):135-141. Ma L,Sun W C,Liu Z G,et al. Expression of cold resistance genes from winter rapeseed of Brassica rapa and B.napus under chilling stress[J]. Chinese Journal of Oil Crop Sciences,2016,38(2):135-141(in Chinese with English abstract).
[22] 丁琼,王华,贾桂霞,等. 沙冬青种子萌发及幼苗生长特性[J]. 植物生态学报,2006,30(4):633-639. Ding Q,Wang H,Jia G X,et al. Seed germination and seedling performance of Ammopiptanthus mongolicus[J]. Journal of Plant Ecology,2006,30(4):633-639(in Chinese with English abstract).
[23] 王俊力,王岩,赵天宏,等. 臭氧胁迫对大豆叶片抗坏血酸-谷胱甘肽循环的影响[J]. 生态学报,2011,31(8):2068-2075. Wang J L,Wang Y,Zhao T H,et al. Effects of ozone on AsA-GSH cycle in soybean leave[J]. Acta Ecologica Sinica,2011,31(8):2068-2075(in Chinese with English abstract).
[24] Jubany-Marí T,Munné-Bosch S,Alegre L,et al. Redox regulation of water stress responses in field-grown plants. Role of hydrogen peroxide and ascorbate[J]. Plant Physiology and Biochemistry,2010,48:351-358.
[25] Mou Z L,Fan W H,Dong X N. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell,2003,113:935-944.
[26] 周艳虹,喻景权,钱琼秋,等. 低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响[J]. 应用生态学报,2003,14(6):921-924. Zhou Y H,Yu J Q,Qian Q Q,et al. Effects of chilling and low light on cucumber seedlings growth and their antioxidative enzyme activities[J]. Chinese Journal of Applied Ecology,2003,14(6):921-924(in Chinese with English abstract).
[27] Hu Y L,Ge Y,Zhang C H,et al. Cadmium toxicity and translocation in rice seedlings are reduced by hydrogen peroxide pretreatment[J]. Plant Growth Regulation,2009,59:51-61.
[28] 姚晓华. 植物抗寒相关功能与调控基因研究进展[J]. 中国农学通报,2013,29(27):141-147. Yao X H. Research advance in the cold-resistance function genes and regulatory genes in plants[J]. Chinese Agricultural Science Bulletin,2013,29(27):141-147(in Chinese with English abstract).
[29] Hodges D M,Andrews C J,Johnson D A,et al. Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines[J]. Journal of Experimental Botany,1997,48(310):1105-1113.
[30] 张腾国,常燕,王娟,等. 油菜BnICE1的克隆及表达分析[J]. 中国农业科学,2013,46(1):205-214. Zhang T G,Chang Y,Wang J,et al. Cloning and expression analysis of a BnICE1 from Brassica napus L.[J]. Scientia Agricultura Sinica,2013,46(1):205-214(in Chinese with English abstract).
[31] Dat J,Vandenabeele S,Vranová E,et al. Dual action of the active oxygen species during plant stress responses[J]. Cell Mol Life Sci,2000,57:779-795.
[32] Orozco-Cárdenas M L,Narváez-Vásquez J,Ryan C A. Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding,systemin,and methyl jasmonate[J]. Plant Cell,2001,13:179-191.

相似文献/References:

[1]董海滨,管荣展.双低油菜华双3号幼苗全长cDNA文库的构建[J].南京农业大学学报,2005,28(3):123.[doi:10.7685/j.issn.1000-2030.2005.03.027]
[2]刘国红,梁明祥,周诗雨,等.甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究[J].南京农业大学学报,2012,35(3):42.[doi:10.7685/j.issn.1000-2030.2012.03.008]
 LIU Guo-hong,LIANG Ming-xiang,ZHOU Shi-yu,et al.The comparative study on different explant regeneration systems in canola‘Nanyanyou 1’(Brassica napus L.)[J].Journal of Nanjing Agricultural University,2012,35(6):42.[doi:10.7685/j.issn.1000-2030.2012.03.008]

备注/Memo

备注/Memo:
收稿日期:2017-02-23。
基金项目:国家公益性行业(农业)科研专项(201403039);国家科技支撑计划项目(2012BAD20B05-03)
作者简介:张曼,硕士研究生。
通信作者:江海东,副教授,主要从事作物生理生态研究,E-mail:hdjiang@njau.edu.cn。
更新日期/Last Update: 1900-01-01