[1]孙荣华,李融梅,何翔,等.水稻白叶枯病菌毒力基因tal17.5病理学功能的研究[J].南京农业大学学报,2017,40(6):1007-1013.[doi:10.7685/jnau.201703008]
 SUN Ronghua,LI Rongmei,HE Xiang,et al.Functional characterization of tal17.5 gene from Xanthomonas oryzae pv. oryzae strain JXOV[J].Journal of Nanjing Agricultural University,2017,40(6):1007-1013.[doi:10.7685/jnau.201703008]
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水稻白叶枯病菌毒力基因tal17.5病理学功能的研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年6期
页码:
1007-1013
栏目:
出版日期:
2017-11-10

文章信息/Info

Title:
Functional characterization of tal17.5 gene from Xanthomonas oryzae pv. oryzae strain JXOV
作者:
孙荣华 李融梅 何翔 宋从凤
南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095
Author(s):
SUN Ronghua LI Rongmei HE Xiang SONG Congfeng
College of Plant Protection/Key Labroatory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
关键词:
稻黄单胞菌水稻致病变种JXOV菌株tal17.5基因PXO99A菌株毒力因子
Keywords:
Xanthomonas oryzae pv. oryzaeJXOV straintal17.5 genePXO99A strainvirulence
分类号:
S432.1
DOI:
10.7685/jnau.201703008
摘要:
[目的] 类转录激活蛋白(transcription activator-like effectors,TALE)是稻黄单胞菌水稻致病变种(Xanthomonas oryzae pv. oryzae,Xoo)中的一类重要致病因子。挖掘新的毒力tal基因,对揭示水稻白叶枯病菌与寄主植物的互作关系具有重要意义。[方法] 从水稻白叶枯病菌JXOV菌株中克隆了1个中间串联重复区含17.5个重复单元的tal基因,命名为tal17.5。将tal17.5转入Xoo菌株PXO99A中,在寄主水稻上研究了其致病力作用,将tal17.5转入ME2中研究了其诱导相关OsSWEET基因的表达情况;在非寄主烟草上研究了其对烟草防卫反应功能的影响。[结果] tal17.5导入PXO99A之后增强了PXO99A在所测试的22个水稻品种中的11个品种上的致病力,并减弱了PXO99A在烟草上的防卫反应,能够一定程度地诱导OsSWEET11和其他5个OsSWEET基因的表达。[结论] TAL17.5通过抑制植物的防卫反应发挥一定的毒力作用。
Abstract:
[Objectives] Transcription activator-like effectors (TALE)are important factors in Xanthomonas oryzae pv. oryzae (Xoo). This paper aims to explore new virulence tal genes and identify their pathological functions.[Methods] We cloned a tal gene named tal17.5 from Xoo JXOV strain, which encoded a typical TALE with 17.5 tandom repeat units. tal17.5 was transformed into ME2 and PXO99A respectively by electroporation. Leaf inoculation and quantitative real-time polymerase chain reaction in host plant and callose deposition induction in non-host tobacco were used in this study to compare the virulence of the transformant with wildtype.[Results] The results showed that the tal17.5 gene significantly increased the PXO99A virulence in 11 out of 22 tested rice varieties and suppressed the defense response in non-host Nicotiana benthamiana. Besides it enhanced six OsSWEET genes expression level in rice variety ‘IRBB10’.[Conclusions] TAL17.5 may contribute as a virulence factor through suppressing the defense response of host plant rice.

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备注/Memo

备注/Memo:
收稿日期:2017-03-06。
基金项目:国家自然科学基金项目(31471744);国家公益性行业(农业)科研专项(201303015)
作者简介:孙荣华,硕士研究生。
通信作者:宋从凤,教授,主要从事分子植物病理学研究,E-mail:cfsong@njau.edu.cn。
更新日期/Last Update: 1900-01-01