FU Yuanyuan,CHEN Kunlin,WANG Zhenyun,et al.Lipolysis mechanism of Chemerin in bovine intramuscular mature adipocytes[J].Journal of Nanjing Agricultural University,2018,41(1):120-125.[doi:10.7685/jnau.201703100]





Lipolysis mechanism of Chemerin in bovine intramuscular mature adipocytes
付园园1 陈坤琳1 王振云1 周光宏2 李惠侠1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 南京农业大学肉品加工与质量控制教育部重点实验室, 江苏 南京 210095
FU Yuanyuan1 CHEN Kunlin1 WANG Zhenyun1 ZHOU Guanghong2 LI Huixia1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Key Laboratory of Meat Products Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Chemerinintramuscular mature adipocyteslipolysis mechanismbovine
[目的]本试验旨在分析脂肪自分泌因子Chemerin在牛肌内成熟脂肪细胞脂解中的作用及调控机制。[方法]体外培养牛肌内成熟脂肪细胞,采用油红O染色与抽提法检测成熟脂肪细胞脂解过程中脂肪含量变化,通过比色法测定细胞中甘油及游离脂肪酸的释放量;采用RT-qPCR及Western blot技术分析脂解关键基因PPARα、LPLHSL mRNA及蛋白的表达情况。[结果]在牛肌内成熟脂肪细胞脂解过程中,细胞中Chemerin及其受体CMKLR1 mRNA表达量显著降低(P<0.05);Chemerin处理牛肌内成熟脂肪细胞后,细胞内脂肪含量减少,成熟脂肪细胞快速脂解为去分化的前体脂肪细胞,细胞中甘油和游离脂肪酸的释放量增加。脂解关键基因PPARα、LPLHSL mRNA及蛋白表达水平与对照组相比极显著升高(P<0.01)。[结论]Chemerin通过上调脂肪细胞脂解关键基因表达,促进了牛肌内成熟脂肪细胞中脂肪的分解,进而可降低肌内脂肪含量。
[Objectives]The aim of this study is to investigate effects and molecular mechamisms of Chemerin in the lipolysis of bovine intramuscular mature adipocytes. [Methods]Bovine intramuscular mature adipocytes were cultured and treated by Chemerin in vitro,and fat content and cell morphological change of the mature adipocyte in the process of lipolysis were measured by oil red O staining. The release of glycerol and free fatty acids was measured by colorimetry,and mRNA and protein expression of key lipolytic genes such as peroxisome proliferator-activated receptor α(PPARα),lipoprotein lipase(LPL) and hormone-sensitive lipase(HSL) were measured by real-time quantitative PCR(RT-qPCR) and Western blot. [Results]mRNA expression of Chemerin and CMKLR1 were significantly decreased in the process of lipolysis(P<0.05). Moreover,in the Chemerin-treatment group,there are less lipid droplets in cells,and the fat in mature adipocytes was accelerated to decompose into glycerol and fatty acid. Mature adipocytes de-differentiate into fibroblast-like pre-adipocytes faster than in control cells. In addition,mRNA and protein expression of PPARα,LPL and HSL were significantly up-regulated by Chemerin during mature adipocytes steatolysis compared to control group(P<0.01). [Conclusions]Through up-regulation the expression of key lipolysis genes,Chemerin promoted the decomposition of fat in bovine intramuscular mature adipocytes,which can reduce the content of intramuscular fat.


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更新日期/Last Update: 1900-01-01