ZHANG Feixue,YU Zhanghong,LIU Kunyu,et al.Cloning and expression analysis of aldo-keto reductase gene BcAKR4C9 from non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2018,41(2):240-247.[doi:10.7685/jnau.201705022]





Cloning and expression analysis of aldo-keto reductase gene BcAKR4C9 from non-heading Chinese cabbage
张飞雪 虞章红 刘坤宇 文锴 王建军 侯喜林 李英
南京农业大学园艺学院/作物遗传与种质创新国家重点实验室/农业部华东地区园艺作物生物学 与种质创新重点实验室, 江苏 南京 210095
ZHANG Feixue YU Zhanghong LIU Kunyu WEN Kai WANG Jianjun HOU Xilin LI Ying
College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Chi
non-heading Chinese cabbagealdo-keto reductase AKR4C subfamilysequence analysisabiotic stress
[目的]本文旨在探究醛酮还原酶AKR4C亚家族BcAKR4C9基因在不同激素处理及逆境胁迫中的表达模式,从而分析BcAKR4C9基因在不结球白菜作物中的生长代谢和逆境胁迫中可能发挥的作用。[方法]以不结球白菜‘苏州青’为材料,克隆了BcAKR4C9基因的全长,应用生物信息学分析了其氨基酸序列,并通过实时荧光定量PCR技术分析BcAKR4C9基因在不同组织,高温、低温、NaCl胁迫、伤害胁迫,以及水杨酸、茉莉酸甲酯和脱落酸等处理下的基因表达变化。[结果]序列分析表明,BcAKR4C9基因包含1个长度为948 bp的开放式阅读框(ORF),编码315个氨基酸。氨基酸序列多重比对和进化树分析表明,BcAKR4C9与其他植物AKR4C9基因同源性较高,具有高度保守性。荧光定量PCR分析表明,BcAKR4C9在叶片中的表达量高于根中,高温和低温处理下表达上调,且具有相似的表达模式。在0~20 g·L-1NaCl质量浓度范围内基因相对表达量随NaCl质量浓度的升高而升高,伤害处理后能够迅速产生应激响应,并能够被水杨酸、茉莉酸甲酯和脱落酸诱导表达。[结论]不结球白菜BcAKR4C9基因能够被不同激素诱导表达,并且参与到多种逆境胁迫响应。
[Objectives]The purpose of this study was to explore the expression pattern of BcAKR4C9 gene of AKR4C subfamily under different hormone treatments and stress conditions,so as to analyze the possible role of BcAKR4C9 gene in growth development and stress in non-heading Chinese cabbage. [Methods]The whole cDNA sequence BcAKR4C9 gene was cloned from non-heading Chinese cabbage ‘Suzhouqing’,and its amino acid sequence was analyzed by bioinformatics,and real-time fluorescence quantitative PCR technology was used to explore its expression pattern under different tissues,different stress conditions(high temperature,low temperature,NaCl stress,wound stress) and different hormone treatments(salicylic acid,methyl jasmonate and abscisic acid). [Results]The sequence analysis showed that the BcAKR4C9 gene contained an open reading frame(ORF) with a length of 948 bp,encoding 315 amino acids. The amino acid sequence multiple alignment and mapping phylogenetic tree showed that BcAKR4C9 gene was highly homologous to the AKR4C9 genes from other plants and highly conserved. Real-time fluorescence quantitative PCR analysis showed that the expression of BcAKR4C9 gene in the leaves was higher than that in the roots,and it was up-regulated at high temperature and low temperature and had similar expression patterns. Its expression increased with the increase of salt concentration from 0 to 20 g·L-1. The expression of BcAKR4C9 also could be induced by salicylic acid,methyl jasmonate and abscisic acid. [Conclusions]The non-heading Chinese cabbage BcAKR4C9 gene can be induced by different hormones and is involved in a variety of stress responses.


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更新日期/Last Update: 1900-01-01