[1]李荣阳,唐小川,徐世永,等.明胶对鸡胚盘细胞的增殖和多功能性的影响[J].南京农业大学学报,2018,41(2):364-369.[doi:10.7685/jnau.201703030]
 LI Rongyang,TANG Xiaochuan,XU Shiyong,et al.Effects of gelatin on proliferation and pluripotency of chicken blastodermal cells[J].Journal of Nanjing Agricultural University,2018,41(2):364-369.[doi:10.7685/jnau.201703030]
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明胶对鸡胚盘细胞的增殖和多功能性的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年2期
页码:
364-369
栏目:
出版日期:
2018-03-27

文章信息/Info

Title:
Effects of gelatin on proliferation and pluripotency of chicken blastodermal cells
作者:
李荣阳1 唐小川2 徐世永3 陈清3 吴望军1 刘红林1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 广西大学动物科学技术学院, 广西 南宁 530000;
3. 金陵科技学院动物科学与技术学院, 江苏 南京 211169
Author(s):
LI Rongyang1 TANG Xiaochuan2 XU Shiyong3 CHEN Qing3 WU Wangjun1 LIU Honglin1
1. College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. College of Animal Sciences and Technology, Guangxi University, Nanning 530000, China;
3. College of Animal Sciences and Technology, Jinling Inst
关键词:
鸡胚盘细胞明胶增殖多功能性
Keywords:
chicken blastodermal cells (cBC)gelatinproliferationpluripotency
分类号:
S831.2
DOI:
10.7685/jnau.201703030
摘要:
[目的]本试验旨在解决鸡胚盘细胞(cBC)不易贴壁的问题及应用E8培养基建立新的鸡胚盘细胞培养体系。[方法]在E8培养基基础上,应用10 g·L-1明胶和人重组玻连蛋白(hVTN)包被培养板,对比其在促进鸡胚盘细胞贴壁速度、促进增殖和维持多功能性方面的差异。[结果]使用10 g·L-1明胶包被培养板后,鸡胚盘细胞12 h内即可迅速贴壁。10 g·L-1明胶处理组中5-乙炔基-2’-脱氧尿嘧啶核苷(EdU)阳性细胞率显著高于对照组(P<0.01),并且能够抑制凋亡基因P21、Fas的mRNA表达(P<0.05),进而促进细胞增殖,并且能够促进多功能基因Nanog、PouV的表达来维持cBC的多功能性。[结论]10 g·L-1明胶可以作为一种新的包被试剂来进行cBC的培养,与人重组玻连蛋白相比,明胶体现了更高的性价比,具有较高的应用价值。
Abstract:
[Objectives]This paper aims to overcome the difficulties of adherence of chicken blastodermal cells(cBC) and to establish a new culture system for chicken blastodermal cells by using E8 medium. [Methods]By using 10 g·L-1 gelatin-coated and human recombinant vitronectin(hVTN) -coated wells,we compared with each other in the adherent rate and maintaining pluripotency of chicken blastodermal cells. [Results]Treated by 10 g·L-1 gelatin on the wells,chicken blastodermal cells could be pasted to the wells within 12 hours. 5-ethynyl-2’-deox-youridine(EdU) positive cells in the 10 g·L-1 gelatin treatment group were significantly higher than those in the control(P<0.01),and could inhibit the mRNA expression of P21 and Fas(P<0.05),and thus promote cell proliferation,and promote the expression of multifunctional genes(Nanog,PouV) to maintain pluripotency of cBC. [Conclusions]10 g·L-1 gelatin can be used as a new embedding reagent for culturing cBC. And compared with the hVTN,treated by 10 g·L-1 gelatin on the wells has higher cost performance and higher application value.

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备注/Memo

备注/Memo:
收稿日期:2017-03-22。
基金项目:“南京创业人才321计划”项目
作者简介:李荣阳,硕士研究生。
通信作者:刘红林,教授,博导,主要从事动物遗传育种与繁殖研究,E-mail:liuhonglin@njau.edu.cn。
更新日期/Last Update: 1900-01-01