[1]吴虎虎,彭麟,季辉,等.鸡饲料中氢溴酸常山酮含量的反相HPLC检测方法的建立[J].南京农业大学学报,2018,41(2):377-381.[doi:10.7685/jnau.201701038]
 WU Huhu,PENG Lin,JI Hui,et al.Development of RP-HPLC method for determination of halofuginone hydrobromide in chicken feeds[J].Journal of Nanjing Agricultural University,2018,41(2):377-381.[doi:10.7685/jnau.201701038]
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鸡饲料中氢溴酸常山酮含量的反相HPLC检测方法的建立()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年2期
页码:
377-381
栏目:
出版日期:
2018-03-27

文章信息/Info

Title:
Development of RP-HPLC method for determination of halofuginone hydrobromide in chicken feeds
作者:
吴虎虎 彭麟 季辉 刘晓晓 滕佩 江善祥
南京农业大学动物医学院, 江苏 南京 210095
Author(s):
WU Huhu PENG Lin JI Hui LIU Xiaoxiao TENG Pei JIANG Shanxiang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
关键词:
氢溴酸常山酮鸡饲料反相高效液相色谱法
Keywords:
halofuginone hydrobromidechickens feedsreversed-phase HPLC
分类号:
S816.75
DOI:
10.7685/jnau.201701038
摘要:
[目的]本文旨在建立检测饲料中氢溴酸常山酮含量的固相萃取-反相高效液相色谱法。[方法]在鸡饲料中添加一定浓度氢溴酸常山酮,经100 g·L-1碳酸钠溶液碱化后用乙酸乙酯提取饲料中氢溴酸常山酮,0.125 mol·L-1乙酸铵缓冲液萃取,HLB柱净化后供HPLC测定。流动相为体积比5:3:12的乙腈、0.25 mol·L-1乙酸铵缓冲液、水溶液(冰乙酸调pH4.3),检测波长243 nm。[结果]该方法对鸡饲料中氢溴酸常山酮的检测限为0.05 μg·g-1,定量限为0.2 μg·g-1。在0.2~15 μg·g-1范围内,线性关系良好,其相关系数大于0.999,在0.2、1.0、5.0和15.0 μg·g-1添加水平下,鸡饲料中氢溴酸常山酮的回收率为87%~113%,日内变异系数为1.1%~17%,日间变异系数为7.1%~12%。[结论]该方法具有良好的线性关系和较高的精密度、回收率,可以简便、灵敏、快速地检测鸡饲料中氢溴酸常山酮含量。
Abstract:
[Objectives]A solid-phase extraction and reversed-phase high performance liquid chromatography(SPE-RP HPLC) method for determination of halofuginone hydrobromide in chicken feeds was developed. [Methods]The chicken feed added to halofuginone hydrobromide was alkalization by 100 g·L-1 sodium carbonate solution and then was successively extracted by ethyl acetate,0.125 mol·L-1 ammonium acetate buffer liquid-liquid extracted,and was cleaned up via HLB column before detection by HPLC. The mobile phase consisted of acetonitrile,0.25 mol·L-1 of ammonium acetate buffer and water(the volume ratio is 5:3:12),adjusted to pH value equal to 4.3 with glacial acetic acid. Halofuginone hydrobromide was monitored by UV detector at 243 nm. [Results]The result showed that,the limit of detection and quantification was 0.05 μg·g-1 and 0.2 μg·g-1,respectively. Among the concentration range from 0.2 to 15 μg·g-1,the correlation coefficient is greater than 0.999,a good linear relation. At 0.2,1.0,5.0,15.0 μg·g-1 levels,the rate recoveries of 87%-113% were obtained,and within-day and between-day cofficents of variation were 1.1%-17% and 7.1%-12%,respectively. [Conclusions]This method can meet the requirements of sensitivity,precision and accuracy,which can be applied for quick and efficient determination of halofuginone hydrobromide in chicken feeds.

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备注/Memo

备注/Memo:
收稿日期:2017-01-20。
作者简介:吴虎虎,硕士研究生。
通信作者:江善祥,教授,主要从事新兽药研发、药物代谢、环境生态毒理学研究,E-mail:jiangshanxiang@163.com。
更新日期/Last Update: 1900-01-01