ZHANG Fengge,HUO Yunqian,SUN Yi,et al.Effect of consecutive biofertilizer application on aboveground biomass and management of soil microflora in grassland[J].Journal of Nanjing Agricultural University,2018,41(2):382-388.[doi:10.7685/jnau.201708004]





Effect of consecutive biofertilizer application on aboveground biomass and management of soil microflora in grassland
张风革1 霍云倩1 孙艺2 肖燕1 杨高文1 张英俊13
1. 南京农业大学草业学院, 江苏 南京 210095;
2. 张家港市耕地质量保护站, 江苏 苏州 215600;
3. 中国农业大学动物科学技术学院, 北京 100193
ZHANG Fengge1 HUO Yunqian1 SUN Yi2 XIAO Yan1 YANG Gaowen1 ZHANG Yingjun13
1. College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China;
2. Zhangjiagang Station of Agro-Environmental Monitoring and Protection, Suzhou 215600, China;
3. College of Animal Science and Technology, China Agricultu
biofertilizergrassland biomasssoil propertieshigh-throughput sequencingsoil microfloraPearson correlation coefficients
[目的]本文旨在明确施用生物有机肥对草地生产力的提升效果,从地下部角度分析并阐明草地生产力提高的微生物生态学机制,为构建高产草地土壤的微生物区系提供理论支撑。[方法]在呼伦贝尔地区采用原位定位试验(连续2年施肥)比较了不同施肥方式(不施肥、普通有机肥和生物有机肥)对草地生物量的影响,并采用高通量测序的方法测定了土壤微生物区系的变化。[结果]与不施肥(CK)或者普通有机肥(OF,9 000 kg·hm-2)相比,施用生物有机肥(BOF,9 000 kg·hm-2)可以显著提高草地地上部生物量。非量度多尺度(NMDS)分析表明不同施肥处理间(CK、OF和BOF)土壤细菌群落结构差异不明显,而真菌群落结构差异显著。施用生物有机肥显著增加了Archaeorhizomyces和木霉属(Trichoderma)的相对丰度,而显著降低了Ophiosphaerella的相对丰度。同时,土壤理化性质和微生物属的相关关系表明,土壤有效磷含量与Archaeorhizomyces和木霉属的相对丰度呈显著正相关,而与Ophiosphaerella呈显著负相关。因此,增加土壤有效磷含量有利于提高草地地上部生物量。[结论]连续施用生物有机肥可以通过调节土壤微生物群落结构和组成来达到提高草地生物量的目的。
[Objectives]Our study aims to understand the promotion effect of biofertilizer on grassland biomass,and demonstrate the potential soil microecological regulation mechanisms,providing an important theoretical basis for constructing high-yield soil rhizosphere microflora. [Methods]We conducted a 2-year in situ fertilization experiment in meadow steppe grasslands in Hulunber, Inner Mongolia,China to evaluate the impacts of different fertilization regime(non-amend fertilization,organic fertilizer,and biofertilizer) on grassland biomass,and the high-throughput sequencing was employed to monitor the potential variation of soil microflora. [Results]The aboveground biomass significantly increased in plots fertilized with biofertilizer(BOF,9 000 kg·hm-2) compared with plots that received organic fertilizer(OF,9 000 kg·hm-2) or no fertilizer(CK). Nonmetric multidimensional scaling(NMDS) revealed that bacterial community structure was relatively similar in the soils of different treatments,while soil fungal community structure was significantly separated by different fertilization regime. BOF application increased the relative abundances of Archaeorhizomyces and Trichoderma,but decreased that of Ophiosphaerella. The correlations between soil properties and microbial genera showed that a higher soil available P might be beneficial to the aboveground biomass by increasing the abundances of Archaeorhizomyces and Trichoderma and decreasing Ophiosphaerella in the soil. [Conclusions]Consecutive biofertilizer application can improve the aboveground biomass by manipulating the soil microbial community structure and composition in grasslands.


[1] 刘辉,王凌云,刘忠珍,等. 我国畜禽粪便污染现状与治理对策[J]. 广东农业科学,2010(6):213-216. Liu H,Wang L Y,Liu Z Z,et al. Pollution condition and cure countermeasures of excrements from livestock and poultry[J]. Guangdong Agricultural Sciences,2010(6):213-216(in Chinese with English abstract).
[2] Moral R,Paredes C,Bustamante M A,et al. Utilisation of manure composts by high-value crops:safety and environmental challenges[J]. Bioresource Technology,2009,100(22):5454-5460.
[3] 杨兴明,徐阳春,黄启为,等. 有机(类)肥料与农业可持续发展和生态环境保护[J]. 土壤学报,2009,45(5):925-932. Yang X M,Xu Y C,Huang Q W,et al. Organic-like fertilizers and its relation to sustainable development of agriculture and protection of eco-environment[J]. Acta Pedologica Sinica,2009,45(5):925-932(in Chinese with English abstract).
[4] Masunaka A,Hyakumachi M,Takenaka S. Plant growth-promoting fungus,Trichoderma koningi suppresses isoflavonoid phytoalexin vestitol production for colonization on/in the roots of Lotus japonicas[J]. Microbes and Environments,2011,26:128-134.
[5] Hoyos-Carvajal L,Orduz S,Bissett J. Growth stimulation in bean(Phaseolus vulgaris L.)by Trichoderma[J]. Biocontrol,2009,51:409-416.
[6] Zhang F G,Yuan J,Yang X M,et al. Putative Trichoderma harzianum mutant promotes cucumber growth by enhanced production of indole acetic acid and plant colonization[J]. Plant and Soil,2013,368:433-444.
[7] 梁昌聪,刘磊,张建华,等. 绿色木霉菌H06固体浅盘发酵工艺优化[J]. 菌物学报,2014,33(6):1313-1326. Liang C C,Liu L,Zhang J H,et al. Optimization of shallow tray fermentation process of Trichoderma viride H06[J]. Mycosystema,2014,33(6):1313-1326(in Chinese with English abstract).
[8] IUSS Working Group WRB. International soil classification system for naming soils and creating legends for soil maps[M]//World Soil Resources Reports No.106. Rome:FAO,2015:153-154.
[9] Caporaso J G,Kucynski J,Stombaugh J. QⅡME allows analysis of high-through-put community sequencing data[J]. Nature Methods,2010,7:335-336.
[10] Yan Y,Eiko E K,de Mattias H,et al. Functional traits dominate the diversity-related selection of bacterial communities in the rhizosphere[J]. The ISME Journal,2016,11(1):56-66.
[11] 宝音陶格涛,刘美玲,包青海,等. 氮素添加对典型草原区割草场植物群落结构及草场质量指数的影响[J]. 草业学报,2011,20(1):7-14. Baoyin T G T,Liu M L,Bao Q H,et al. Effect of N addition on plant community structure and index of grassland quality of typical steppe mown grassland[J]. Acta Prataculturae Sinica,2011,20(1):7-14(in Chinese with English abstract).
[12] Yedidia I,Srivastva A K,Kapulnik Y,et al. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants[J]. Plant and Soil,2001,235(2):235-242.
[13] Shukla S K,Yadav R L,Suman A,et al. Improving rhizospheric environment and sugarcane ratoon yield through bio-agents amended farm yard manure in udic ustochrept soil[J]. Soil and Tillage Research,2008,99:158-168.
[14] Vo Arírková J,Baldrian P. Fungal community on decomposing leaf litter undergoes rapid successional changes[J]. The ISME Journal,2013,7:477-486.
[15] Chen L H,Huang X Q,Zhang F G,et al. Application of Trichoderma harzianum SQR-T037 bio-organic fertiliser significantly controls Fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber[J]. Journal of the Science of Food and Agriculture,2012,92:2465-2470.
[16] Luo J,Ran W,Hu J,et al. Application of bio-organic fertilizer significantly affected fungal diversity of soils[J]. Soil Science Society of American Journal,2010,74(6):2039-2048.
[17] Zhang F G,Zhu Z,Yang X M,et al. Trichoderma harzianum T-E5 significantly affects cucumber root exudates and fungal community in the cucumber rhizosphere[J]. Applied Soil Ecology,2013,72:41-48.
[18] Marschner P,Kandeler E,Marschner B. Structure and function of the soil microbial community in a long-term fertilizer experiment[J]. Soil Biology Biochemistry,2003,35(3):453-461.
[19] Gravel V,Antoun H,Tweddell R J. Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride:possible role of indole acetic acid(IAA)[J]. Soil Biology Biochemistry,2007,39:1968-1977.
[20] Bae H,Sicher R C,Kim M S,et al. The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao[J]. Journal of Experimental Botany,2009,60:3279-3295.
[21] 程玲娟.深绿木霉对牧草促生作用及其作用机制研究[D]. 兰州:甘肃农业大学,2008. Cheng L J. Studies on the promotion and effect mechanism of Trichoderma aureoviride on the grazing[D]. Lanzhou:Gansu Agricultural University,2008(in Chinese with English abstract).
[22] Rosling A,Cox F,Cruz-Martinez K,et al. Archaeorhizomycetes:unearthing an ancient class of ubiquitous soil fungi[J]. Science,2011,333:876-879.
[23] Hciii W,Skinner D Z,Tisserat N A. Geographic distribution and genetic diversity of three Ophiosphaerella species that cause spring dead spot of bermudagrass[J]. Plant Disease,2007,83(12):1160-1166.
[24] Venkatasubbaiah P,Tisserat N A,Chilton W S. Metabolites of Ophiosphaerella herpotricha,a cause of spring dead spot of bermudagrass[J]. Mycopathologia,1994,128(3):155-159.


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 GAO Xue-lian,DENG Kai-ying,ZHANG Peng,et al.Effects of different bio-organic fertilizers on fusarium wilt control and on the rhizosphere microbial community with muskmelon[J].Journal of Nanjing Agricultural University,2012,35(2):55.[doi:10.7685/j.issn.1000-2030.2012.06.010]
 ZHANG Guo-yi,DING Chuan-yu,REN Li-xuan,et al.The synergetic inhibition effect of arbuscular mycorrhizal fungi cooperated Bacillus vallismortis organic fertilizer on cotton Verticillium wilt[J].Journal of Nanjing Agricultural University,2012,35(2):68.[doi:10.7685/j.issn.1000-2030.2012.06.012]
 DENG Kaiying,LING Ning,ZHANG Peng,et al.Effects of bio-organic fertilizers on the growth and rhizosphere microflora of watermelon plants in nursery pots[J].Journal of Nanjing Agricultural University,2013,36(2):103.[doi:10.7685/j.issn.1000-2030.2013.02.017]
 ZHANG Peng,WEI Zhong,ZHU Zhen,et al.Effect of a bio-organic fertilizer on microbial flora and Ralstonia solanacearum population in rhizosphere soils of continuous cropping tomato and pepper[J].Journal of Nanjing Agricultural University,2013,36(2):77.[doi:10.7685/j.issn.1000-2030.2013.04.013]
 MA Tiantian,CAI Feng,DING Chuanyu,et al.Biological effects of special bio-organic fertilizer on yam[J].Journal of Nanjing Agricultural University,2013,36(2):83.[doi:10.7685/j.issn.1000-2030.2013.04.014]
 CAO Liangliang,ZHANG Miao,SHI Juanjuan,et al.Adding protein materials as solid-state fermentation media for producing bio-organic fertilizers with functional microbes[J].Journal of Nanjing Agricultural University,2014,37(2):85.[doi:10.7685/j.issn.1000-2030.2014.02.014]
 CHEN Xi,ZHAO Shuang,SHI Yadong,et al.Effects of bio-organic fertilizer application on the continuous cropping obstacles of Chrysanthemum morifolium Tzvel.‘Chuju’[J].Journal of Nanjing Agricultural University,2015,38(2):50.[doi:10.7685/j.issn.1000-2030.2015.01.008]
 WEI Zhong,HU Jie,DONG Yue,et al.Screen of antagonists against Ralstonia solanacearum by using rapeseed cake compost as a selected medium[J].Journal of Nanjing Agricultural University,2015,38(2):424.[doi:10.7685/j.issn.1000-2030.2015.03.011]
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