LI Fangfang,YANG Na,QIAN Meng,et al.Auxin is involved in triacontanol-induced lateral root development in Arabidopsis thaliana[J].Journal of Nanjing Agricultural University,2018,41(3):473-480.[doi:10.7685/jnau.201709016]





Auxin is involved in triacontanol-induced lateral root development in Arabidopsis thaliana
李芳芳 杨娜 钱猛 甘立军
南京农业大学生命科学学院, 江苏 南京 210095
LI Fangfang YANG Na QIAN Meng GAN Lijun
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
auxinlateral roottriacontanolArabidopsis thaliana
[目的]本文旨在研究植物生长调节剂三十烷醇对拟南芥侧根发育的影响,揭示其调控侧根发育的机制,为生产上的使用提供理论依据。[方法]以野生型拟南芥、生长素不敏感型突变体为试验材料,外源三十烷醇处理生长5 d的幼苗,分析侧根数目、侧根密度、侧根原基数量、侧根原基密度、细胞周期调控的关键基因的表达、根部内源吲哚乙酸(indole-3-acetic acid,IAA)含量、生长素合成关键基因表达等指标。[结果]外源三十烷醇处理拟南芥的幼苗,能够诱导侧根的产生,0.20、0.50和1.00 μmol·L-1三十烷醇处理8 d,侧根密度分别增加了59.0%、97.9%和54.2%;0.5 μmol·L-1三十烷醇处理后阶段A的侧根(此时包含3层细胞)原基密度增加了67.8%。外源三十烷醇处理增加根部IAA的含量,上调参与生长素合成的关键酶基因表达,增强根尖和不同发育阶段侧根生长素响应报告基因DR5∶β-glucuronidaseGUS)和IAA2∶GUS的表达。生长素运输抑制剂三碘苯甲酸(2,3,5-triiodobenzoic acid,TIBA)及萘基邻氨甲酰苯甲酸(1-naphthylphthalamic acid,NPA)和作用抑制剂p-chlorophenoxy isobutyric acid(PCIB)的添加抑制了三十烷醇诱导的侧根发育;生长素不敏感型突变体tir1-1axr1-3对三十烷醇缺乏响应,而aux1-7eir1-1对三十烷醇的响应弱于野生型。[结论]三十烷醇能够通过促进侧根原基的从头形成来增加侧根的密度,其诱导侧根发育依赖生长素的途径。
[Objectives] The aim of the paper is to explore the possible mechanism of triacontanol(TRIA)in the regulation of lateral root(LR)development, and to provide theoretical basis for the practice. [Methods] Using wild type(WT), auxin-insensitive mutant seedlings as materials, the 5-day-old seedlings were treated with different concentrations of TRIA and the effect of TRIA on LR formation was analyzed. [Results] TRIA treatment induced LR formation markedly, and the increase in the LR density was positively correlated with TRIA concentration. After treatment with 0.20, 0.50, and 1.00 μmol·L-1 TRIA for 8 d, the density of LR increased by 59.0%, 97.9% and 54.2%, respectively. The density of stage A lateral root primordium increased by 67.8% under 0.5 μmol·L-1 TRIA treatment compared with the control. TRIA application significantly increased the indole-3-acetic acid(IAA)level, transcript levels of many IAA biosynthesis genes and the expression levels of DR5:GUS and IAA2:GUS in root. Auxin transport inhibitors 2, 3, 5-triiodobenzoic acid(TIBA)and 1-naphthylphthalamic acid(NPA), and the auxin action inhibitor p-chlorophenoxy isobutyric acid(PCIB)each inhibited TRIA-mediated LR formation dramatically in WT seedlings. Further genetic studies revealed that LR formation in tir1-1 and axr1-3 mutants was insensitive to TRIA treatment, but LR formation was less sensitive in aux1-7 and eir1-1 mutants than in WT plants. [Conclusions] Our results showed that TRIA treatment promoted LR formation by inducing de novo formation of lateral root primordium(LRP)in Arabidopsis seedlings and auxin-dependent pathway participated in the regulation of LR formation when Arabidopsis seedlings were subjected to exogenous TRIA application.


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