[1]李芳芳,杨娜,钱猛,等.生长素参与三十烷醇诱导的拟南芥侧根发育[J].南京农业大学学报,2018,41(3):473-480.[doi:10.7685/jnau.201709016]
 LI Fangfang,YANG Na,QIAN Meng,et al.Auxin is involved in triacontanol-induced lateral root development in Arabidopsis thaliana[J].Journal of Nanjing Agricultural University,2018,41(3):473-480.[doi:10.7685/jnau.201709016]
点击复制

生长素参与三十烷醇诱导的拟南芥侧根发育()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年3期
页码:
473-480
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Auxin is involved in triacontanol-induced lateral root development in Arabidopsis thaliana
作者:
李芳芳 杨娜 钱猛 甘立军
南京农业大学生命科学学院, 江苏 南京 210095
Author(s):
LI Fangfang YANG Na QIAN Meng GAN Lijun
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
生长素侧根三十烷醇拟南芥
Keywords:
auxinlateral roottriacontanolArabidopsis thaliana
分类号:
Q945
DOI:
10.7685/jnau.201709016
摘要:
[目的]本文旨在研究植物生长调节剂三十烷醇对拟南芥侧根发育的影响,揭示其调控侧根发育的机制,为生产上的使用提供理论依据。[方法]以野生型拟南芥、生长素不敏感型突变体为试验材料,外源三十烷醇处理生长5 d的幼苗,分析侧根数目、侧根密度、侧根原基数量、侧根原基密度、细胞周期调控的关键基因的表达、根部内源吲哚乙酸(indole-3-acetic acid,IAA)含量、生长素合成关键基因表达等指标。[结果]外源三十烷醇处理拟南芥的幼苗,能够诱导侧根的产生,0.20、0.50和1.00 μmol·L-1三十烷醇处理8 d,侧根密度分别增加了59.0%、97.9%和54.2%;0.5 μmol·L-1三十烷醇处理后阶段A的侧根(此时包含3层细胞)原基密度增加了67.8%。外源三十烷醇处理增加根部IAA的含量,上调参与生长素合成的关键酶基因表达,增强根尖和不同发育阶段侧根生长素响应报告基因DR5∶β-glucuronidaseGUS)和IAA2∶GUS的表达。生长素运输抑制剂三碘苯甲酸(2,3,5-triiodobenzoic acid,TIBA)及萘基邻氨甲酰苯甲酸(1-naphthylphthalamic acid,NPA)和作用抑制剂p-chlorophenoxy isobutyric acid(PCIB)的添加抑制了三十烷醇诱导的侧根发育;生长素不敏感型突变体tir1-1axr1-3对三十烷醇缺乏响应,而aux1-7eir1-1对三十烷醇的响应弱于野生型。[结论]三十烷醇能够通过促进侧根原基的从头形成来增加侧根的密度,其诱导侧根发育依赖生长素的途径。
Abstract:
[Objectives] The aim of the paper is to explore the possible mechanism of triacontanol(TRIA)in the regulation of lateral root(LR)development, and to provide theoretical basis for the practice. [Methods] Using wild type(WT), auxin-insensitive mutant seedlings as materials, the 5-day-old seedlings were treated with different concentrations of TRIA and the effect of TRIA on LR formation was analyzed. [Results] TRIA treatment induced LR formation markedly, and the increase in the LR density was positively correlated with TRIA concentration. After treatment with 0.20, 0.50, and 1.00 μmol·L-1 TRIA for 8 d, the density of LR increased by 59.0%, 97.9% and 54.2%, respectively. The density of stage A lateral root primordium increased by 67.8% under 0.5 μmol·L-1 TRIA treatment compared with the control. TRIA application significantly increased the indole-3-acetic acid(IAA)level, transcript levels of many IAA biosynthesis genes and the expression levels of DR5:GUS and IAA2:GUS in root. Auxin transport inhibitors 2, 3, 5-triiodobenzoic acid(TIBA)and 1-naphthylphthalamic acid(NPA), and the auxin action inhibitor p-chlorophenoxy isobutyric acid(PCIB)each inhibited TRIA-mediated LR formation dramatically in WT seedlings. Further genetic studies revealed that LR formation in tir1-1 and axr1-3 mutants was insensitive to TRIA treatment, but LR formation was less sensitive in aux1-7 and eir1-1 mutants than in WT plants. [Conclusions] Our results showed that TRIA treatment promoted LR formation by inducing de novo formation of lateral root primordium(LRP)in Arabidopsis seedlings and auxin-dependent pathway participated in the regulation of LR formation when Arabidopsis seedlings were subjected to exogenous TRIA application.

参考文献/References:

[1] Du Y J,Scheres B. Lateral root formation and the multiple roles of auxin[J]. J Exp Bot,2017,69(2):155-167.
[2] Lavenus J,Goh T,Roberts I,et al. Lateral root development in Arabidopsis:fifty shades of auxin[J]. Trends Plant Sci,2013,18:450-458.
[3] Dubrovsky J G,Sauer M,Napsucialy-Mendivil S,et al. Auxin acts as a local morphogenetic trigger to specify lateral root founder cells[J]. Proc Natl Acad Sci USA,2008,105:8790-8794.
[4] Benková E,Michniewicz M,Sauer M,et al. Local,efflux-dependent auxin gradients as a common module for plant organ formation[J]. Cell,2003,115:591-602.
[5] Swarup,K,Benková E,Swarup R,et al. The auxin influx carrier LAX3 promotes lateral root emergence[J]. Nat Cell Biol,2008,10:946-954.
[6] Péret B,Li G,Zhao J,et al. Auxin regulates aquaporin function to facilitate lateral root emergence[J]. Nat Cell Biol,2012,14:991-998.
[7] Ries S K,Wert V F. Growth response of rice seedlings to triacontanol in light and dark[J]. Planta,1977,135:77-82.
[8] Naeem M,Khan M M A,Moinuddin. Triacontanol:a potent plant growth regulator in agriculture[J]. J Plant Interact,2012,7(2):129-142.
[9] Muthuchelian K,Velayutham M,Nedunchezhian N. Ameliorating effect of triacontanol on acidic mist-treated erythrina variegate seedlings changes in growth and photosynthetic activities[J]. Plant Sci,2003,165:1253-1257.
[10] Chen X,Yuan H,Chen R,et al. Biochemical and photochemical changes in response to triacontanol in rice(Oryza sativa L.)[J]. Plant Growth Regul,2003,40:249-256.
[11] Naeem M,Khan M M A,Moinuddin,et al. Triacontanol stimulates nitrogen-fixation,enzyme activities,photosynthesis,crop productivity and quality of hyacinth bean(Lablab purpureus L.)[J]. Sci Hort,2009,121:389-396.
[12] Shahbaz M,Noreen N,Perveen S. Triacontanol modulates photosynthesis and osmoprotectants in canola(Brassica napus L.)under saline stress[J]. J Plant Interact,2013,8(4):350-359.
[13] Karam E A,Keramat B,Asrar Z,et al. Triacontanol-induced changes in growth,oxidative defense system in coriander(Coriandrum sativum)under arsenic toxicity[J]. Ind J Plant Physiol,2016,21(2):137-142.
[14] Shirakawa M,Ueda H,Shimada T,et al. Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development[J]. Plant J,2010,64:924-935.
[15] Zhang H,Jennings A,Barlow P W,et al. Dual pathways for regulation of root branching by nitrate[J]. Proc Natl Acad Sci USA,1999,96:6529-6534.
[16] Ding X,Cao Y,Huang L,et al. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate-and jasmonate-independent basal immunity in rice[J]. Plant Cell,2008,20:228-240.
[17] Liu W,Li R J,Han T T,et al. Salt stress reduces root meristem size by nitric oxide-mediated modulation of auxin accumulation and signaling in Arabidopsis[J]. Plant Physiol,2015,168:343-356.
[18] Li G,Zhu C,Gan L,et al. GA3 enhances root responsiveness to exogenous IAA by modulating auxin transport and signaling in Arabidopsis[J]. Plant Cell Rep,2015,34:483-494.
[19] Ma X L,Zhu C H,Yang N,et al. γ-Aminobutyric acid addition alleviates ammonium toxicity by limiting ammonium accumulation in rice(Oryza sativa)seedlings[J]. Physiol Plant,2016,158:389-401.
[20] Liu Y,Wang R,Zhang P,et al. The nitrification inhibitor methyl 3-(4-hydroxyphenyl)propionate modulates root development by interfering with auxin signaling via the NO/ROS pathway in Arabidopsis[J]. Plant Physiol,2016,171:1686-1703.
[21] Feng Z,Sun X,Wang G,et al. LBD29 regulates the cell cycle progression in response to auxin during lateral root formation in Arabidopsis thaliana[J]. Ann Bot,2012,110:1-10.
[22] Waqas M,Shahzad R,Khan A L,et al. Salvaging effect of triacontanol on plant growth,thermotolerance,macro-nutrient content,amino acid concentration and modulation of defense hormonal levels under heat stress[J]. Plant Physiol Biochem,2016,99:118-125.
[23] Hangarter R,Ries S. Effect of triacontanol on plant cell cultures in vitro[J]. Plant Physiol,1978,61:855-857.
[24] Giridhar P,Indu E P,Ravishankar G A,et al. Influence of triacontanol on somatic embryogenesis in Coffea arabica L. and Coffea canephora P. ex Fr.[J]. In vitro Cell Dev Biol Plant,2004,40:200-203.
[25] Masubelele N H,Dewitte W,Menges M,et al. D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis[J]. Proc Natl Acad Sci USA,2005,102:15694-15699.
[26] Nieuwland J,Maughan S,Dewitte W,et al. The D-type cyclin CYCD4;1 modulates lateral root density in Arabidopsis by affecting the basal meristem region[J]. Proc Natl Acad Sci USA,2009,106:22528-22533.
[27] Xie Z,Lee E,Lucas J R,et al. Regulation of cell proliferation in the stomatal lineage by the Arabidopsis MYB FOUR LIPS via direct targeting of core cell cycle genes[J]. Plant Cell,2010,22:2306-2321.
[28] Boerjan W,Cervera M T,Delarue M,et al. Superroot,a recessive mutation in Arabidopsis,confers auxin overproduction[J]. Plant Cell,1995,7:1405-1419.
[29] Nakazawa M,Yabe N,Ichikawa T,et al. DFL1,an auxin-responsive GH3 gene homologue,negatively regulates shoot cell elongation and lateral root formation,and positively regulates the light response of hypocotyl length[J]. Plant J,2001,25:213-221.
[30] Raya-González J,Ortiz-Castro R,Ruíz-Herrera L F,et al. PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25 regulates lateral root formation via auxin signaling in Arabidopsis[J]. Plant Physiol,2014,165:880-894.
[31] Khan A,Hossain M T,Park H C,et al. Development of root system architecture of Arabidopsis thaliana in response to colonization by Martelella endophytica YC6887 depends on auxin signaling[J]. Plant Soil,2016,405:81-96.
[32] Bennett M J,Marchant A,Green H G,et al. Arabidopsis AUX1 gene:a permease-like regulator of root gravitropism[J]. Science,1996,273:948-950.
[33] Pérez-Torres C A,López-Bucio J,Cruz-Ramírez A,et al. Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor[J]. Plant Cell,2014,20:3258-3272.

相似文献/References:

[1]张辰明,徐烨红,赵海娟,等.不同氮形态对水稻苗期氮素吸收和根系生长的影响[J].南京农业大学学报,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
[2]王淑敏,侯喜林*,李英,等.芜菁花叶病毒对不结球白菜内源激素含量及代谢相关基因转录水平的影响[J].南京农业大学学报,2011,34(5):13.[doi:10.7685/j.issn.1000-2030.2011.05.003]
 WANG Shu-min,HOU Xi-lin*,LI Ying,et al.Effects of Turnip mosaic virus(TuMV)on endogenous hormones and transcriptional level of related genes in infected non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2011,34(3):13.[doi:10.7685/j.issn.1000-2030.2011.05.003]
[3]张杨,文春燕,赵买琼,等.辣椒根际促生菌的分离筛选及生物育苗基质研制[J].南京农业大学学报,2015,38(6):950.[doi:10.7685/j.issn.1000-2030.2015.06.012]
 ZHANG Yang,WEN Chunyan,ZHAO Maiqiong,et al.Isolation of plant growth promoting rhizobacteria from pepper and development of bio-nursery substrates[J].Journal of Nanjing Agricultural University,2015,38(3):950.[doi:10.7685/j.issn.1000-2030.2015.06.012]
[4]刘晓东,李月,王若仲,等.过表达GH3-5提高拟南芥抗旱的分子机制[J].南京农业大学学报,2016,39(4):557.[doi:10.7685/jnau.201604019]
 LIU Xiaodong,LI Yue,WANG Ruozhong,et al.Molecular mechanism of drought tolerance conferred by overexpression of GH3-5[J].Journal of Nanjing Agricultural University,2016,39(3):557.[doi:10.7685/jnau.201604019]
[5]黄文晓,翁飞,査满荣,等.水稻突变体D12W191多分蘖表型产生与细胞分裂素的关系[J].南京农业大学学报,2016,39(5):711.[doi:10.7685/jnau.201603056]
 HUANG Wenxiao,WENG Fei,ZHA Manrong,et al.Relationship of the multi-tiller phenotype of a rice mutant D12W191 with cytokinin[J].Journal of Nanjing Agricultural University,2016,39(3):711.[doi:10.7685/jnau.201603056]

备注/Memo

备注/Memo:
收稿日期:2017-09-07。
基金项目:国家自然科学基金项目(31501236);中央高校基本科研业务费专项资金(KJQN201639)
作者简介:李芳芳,硕士研究生。
通信作者:甘立军,主要从事植物激素作用机制的研究,E-mail:ganlj@njau.edu.cn。
更新日期/Last Update: 1900-01-01