[1]王利宏,王庆增,鲍建军,等.Hippo信号通道中Lats2基因表达与湖羊肌肉生长发育的关系[J].南京农业大学学报,2018,41(3):519-525.[doi:10.7685/jnau.201705035]
 WANG Lihong,WANG Qingzeng,BAO Jianjun,et al.Relationship between Lats2 gene in Hippo signaling pathway and Hu sheep muscles growth traits[J].Journal of Nanjing Agricultural University,2018,41(3):519-525.[doi:10.7685/jnau.201705035]
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Hippo信号通道中Lats2基因表达与湖羊肌肉生长发育的关系()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年3期
页码:
519-525
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Relationship between Lats2 gene in Hippo signaling pathway and Hu sheep muscles growth traits
作者:
王利宏1 王庆增1 鲍建军1 于嘉瑞1 陈玲2 方明3 袁文龙4 孙伟1
1. 扬州大学动物科学与技术学院, 江苏 扬州 225009;
2. 苏州市畜牧兽医站, 江苏 苏州 215200;
3. 徐州市睢宁县科技局, 江苏 睢宁 221200;
4. 徐州天雨牧业有限公司, 江苏 睢宁 221200
Author(s):
WANG Lihong1 WANG Qingzeng1 BAO Jianjun1 YU Jiarui1 CHEN Ling2 FANG Ming3 YUAN Wenlong4 SUN Wei1
1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Animal Science and Veterinary Medicine Bureau of Suzhou, Suzhou 215200, China;
3. Technology Bureau of Suining County of Xuzhou, Suining 221200, China;
4. Xuzhou Tianyu Sheep Industry Co. Ltd., Suining 221200, China
关键词:
湖羊Lats2基因Hippo通路YAP1肌肉生长相关基因
Keywords:
Hu sheepLats2 geneHippo signal channelYAP1related genes of muscle growth
分类号:
Q418
DOI:
10.7685/jnau.201705035
摘要:
[目的]探究Hippo信号通道中Lats2基因与湖羊肌肉生长发育的关联性。[方法]以湖羊作为试验材料,用RT-qPCR技术分析Lats2基因在不同性别、不同生长阶段(2、60、180日龄)、不同肌肉组织(比目鱼肌、背最长肌、腓肠肌和趾长伸肌)的时空表达规律,及其与Lats1、YAP1(Yes相关蛋白1)基因和肌肉生长相关基因在不同生长阶段的相关性。[结果]根据Hippo信号通道中Lats2基因的时空表达规律可以发现:在不同肌肉组织中,Lats2基因在趾长伸肌中相对表达量最高;在不同生长阶段,Lats2基因在60日龄的表达量最高;在不同性别中,Lats2基因的表达表现为公羊高于母羊。相关性分析结果表明:在2日龄肌肉组织中,Lats2基因的表达与MSTN基因间正相关(P<0.05);在60日龄的肌肉组织中,Lats2基因的表达与Lats1基因正相关(P<0.05);在180日龄肌肉组织中,Lats2基因的表达与MyoG基因间极显著正相关(P<0.01),与YAP1基因间显著负相关(P<0.05)。[结论]Hippo信号通道中Lats2基因的表达受不同性别、年龄和肌肉组织的影响,可能通过下调YAP1基因的表达抑制肌肉生长发育,可作为肌肉生长发育调控研究的候选基因。
Abstract:
[Objectives] The experiment mainly explored the correlation between large tumor suppressor kinase 2 gene(Lats2)in Hippo signal channel and Hu sheep muscle growth by RT-qPCR technique. [Methods] In this study, we analyzed the spatial and temporal expression regulation of Lats2 in Hu sheep with the affection of different gender, growth stages(2, 60 and 180 day-old)and skeletal muscle tissue(soleus muscle, longissimus doris muscle, gastrocnemius muscle, extensor digitorum longus). The correlation of expression in different growth stages within large tumor suppressor kinase 1 gene(Lats1), Yes-associated protein 1 gene(YAP1)and genes, which could affect the growth of muscle tissue(MyoG, MyHCⅠ, MSTN, MyHCⅡA, MyHCⅡX), or the genes related to muscle growth was emphatically discussed. [Results] It suggested that Lats2 in Hippo signal channel had the highest related expression in the extensor digitorumlongus muscle on the analysis of different muscle tissue expression. Affected by different growth stages, Lats2 gene had the highest related expression in the 6-month-old, and higher in the 2-day-old and the 2-month-old. In different genders, the related expression of rams was higher than that of ewes. On the analysis of related expression of Lats2, Lats1, YAP1 and genes that could affect the growth of muscle tissue, we found that Lats2 in 2-day-old muscle tissue had significant positive correlation with MSTN(P<0.05). In 2-day-old muscle tissue, there was a significant positive correlation between Lats2 and Lats1(P<0.05). The expression of Lats2 and MyoG had highly significant positive correlation(P<0.01), and the correlation of the expression between Lats2 and YAP1 was significant negative in 6-month-old muscle tissue(P<0.05). [Conclusions] The expression of Lats2 gene in Hippo signal channel was affected by different gender, growth stages and skeletal muscle tissue. We can down-regulate the expression of YAPA1 to inhibit the muscle growth, which can be a candidate gene of the growth of muscle fiber.

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备注/Memo

备注/Memo:
收稿日期:2017-07-23。
基金项目:科技部家养动物平台项目;江苏省农业重大新品种创新项目(PZCZ201739);江苏省高校自然科学研究重大项目(17KJA230001);江苏高校优势学科建设工程资助项目;江苏省苏北科技计划(BN2014003,BN2015013,BN2015014);江苏省六大高峰人才项目;扬州大学研究生创新工程项目(XKYCX17_060)
作者简介:王利宏,硕士研究生。
通信作者:孙伟,博士,教授,主要从事动物遗传资源与分子标记育种研究,E-mail:dkxmsunwei@163.com。
更新日期/Last Update: 1900-01-01