ZENG Zhenling,WANG Mianzhi,SUN Yongxue.Research advances of bacteriophages in contributing antimicrobial resistance gene in the environment[J].Journal of Nanjing Agricultural University,2018,41(4):598-604.[doi:10.7685/jnau.201712006]





Research advances of bacteriophages in contributing antimicrobial resistance gene in the environment
曾振灵 王勉之 孙永学
华南农业大学国家兽医微生物耐药性风险评估实验室, 广东 广州 510642
ZENG Zhenling WANG Mianzhi SUN Yongxue
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
bacteriophages(or phages)transductionantimicrobial resistant geneshorizontal gene transfer
Bacteriophages(or phages)are the most abundant and diverse biological entities on earth,and the number is 10-100 times higher than that of bacteria. They infect susceptible bacterial hosts by transduction,enter the cells to reproduce or integrate their genes into the bacterial genome in the form of prophage,contributing to their preservation(short-term)or evolution(long-term). In recent years,it had been found that a large number of antimicrobial resistant genes(ARG)in phage genomes were detected and confirmed by quantitative PCR(qPCR)method from different environmental sources. Among these genes,β-lactamases resistance genes(blaTEM, blaCTX-M-groups, blaSHV, blaNDM and blaKPC)and fluoroquinolones resistant genes(qnrA,qnrB and qnrS)had the highest detection rate and absolute abundance. Nowadays,with the continuous progress of high-throughput sequencing methods(metagenomic analysis),the results of metagenomic analysis on different environmental samples also revealed the presence of high levels of ARG. These results suggest that bacteriophage may be a potential vector and important reservoir to enhance the spread of antibiotic resistance. In this study,we will review the diversity and abundary of phage-carrying ARG in different global enviromental samples by qPCR methods and whole genome sequencing methods,and hope to assess their potential hazards and arouse more attention.


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更新日期/Last Update: 1900-01-01