[1]曾振灵,王勉之,孙永学.环境中噬菌体携带耐药基因传播的研究进展[J].南京农业大学学报,2018,41(4):598-604.[doi:10.7685/jnau.201712006]
 ZENG Zhenling,WANG Mianzhi,SUN Yongxue.Research advances of bacteriophages in contributing antimicrobial resistance gene in the environment[J].Journal of Nanjing Agricultural University,2018,41(4):598-604.[doi:10.7685/jnau.201712006]
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环境中噬菌体携带耐药基因传播的研究进展()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年4期
页码:
598-604
栏目:
出版日期:
2018-07-09

文章信息/Info

Title:
Research advances of bacteriophages in contributing antimicrobial resistance gene in the environment
作者:
曾振灵 王勉之 孙永学
华南农业大学国家兽医微生物耐药性风险评估实验室, 广东 广州 510642
Author(s):
ZENG Zhenling WANG Mianzhi SUN Yongxue
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
关键词:
噬菌体转导耐药基因基因水平转移
Keywords:
bacteriophages(or phages)transductionantimicrobial resistant geneshorizontal gene transfer
分类号:
S831.5
DOI:
10.7685/jnau.201712006
摘要:
噬菌体是地球上最丰富和最多样化的生物实体,其数量是细菌的10~100倍。它们感染易感细菌宿主,并通过转导作用在宿主细胞内繁殖或以前噬菌体形式整合自身基因至细菌基因组中,从而有助于它们在细菌宿主中的繁殖(短期)或进化(长期)。利用荧光定量PCR方法(qPCR)对不同环境来源的噬菌体基因进行定量分析,发现其基因组中携带大量的耐药基因(ARG),其中检出率及丰度最高的为耐β-内酰胺抗生素的基因,主要为blaTEMblaCTX-M-groupsblaSHVblaNDMblaKPC,以及耐氟喹诺酮类药物的基因qnrA、qnrBqnrS。且随着高通量测序方法的不断改进,用此方法针对不同样品的宏基因组测序结果分析也同样发现了高水平ARG的存在。这些结果表明噬菌体可能成为潜在的储存载体,促进抗生素耐药性的传播。本文将从荧光定量PCR(qPCR)方法和全基因组测序方法两方面综述全球不同环境样本中噬菌体基因组携带ARG的情况以及其多样性和丰度的研究进展,并讨论其潜在危害性,希望引起更多的关注。
Abstract:
Bacteriophages(or phages)are the most abundant and diverse biological entities on earth,and the number is 10-100 times higher than that of bacteria. They infect susceptible bacterial hosts by transduction,enter the cells to reproduce or integrate their genes into the bacterial genome in the form of prophage,contributing to their preservation(short-term)or evolution(long-term). In recent years,it had been found that a large number of antimicrobial resistant genes(ARG)in phage genomes were detected and confirmed by quantitative PCR(qPCR)method from different environmental sources. Among these genes,β-lactamases resistance genes(blaTEM, blaCTX-M-groups, blaSHV, blaNDM and blaKPC)and fluoroquinolones resistant genes(qnrA,qnrB and qnrS)had the highest detection rate and absolute abundance. Nowadays,with the continuous progress of high-throughput sequencing methods(metagenomic analysis),the results of metagenomic analysis on different environmental samples also revealed the presence of high levels of ARG. These results suggest that bacteriophage may be a potential vector and important reservoir to enhance the spread of antibiotic resistance. In this study,we will review the diversity and abundary of phage-carrying ARG in different global enviromental samples by qPCR methods and whole genome sequencing methods,and hope to assess their potential hazards and arouse more attention.

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备注/Memo

备注/Memo:
收稿日期:2017-12-06。
基金项目:广东省高校重大科研项目(2017KZDXM006);国家重点基础研究计划项目(2013CB127203);国家自然科学基金项目(31772803)
作者简介:曾振灵,教授,主要从事兽医药理学与毒理学研究,E-mail:zlzeng@scau.edu.cn。
通信作者:曾振灵,教授,主要从事兽医药理学与毒理学研究,E-mail:zlzeng@scau.edu.cn
更新日期/Last Update: 1900-01-01