[1]吴潇,陈杨杨,石新杰,等.喷施外源激素对‘玉露香’梨叶片表皮蜡质组分、结构及渗透性的影响[J].南京农业大学学报,2018,41(4):647-654.[doi:10.7685/jnau.201710007]
　WU Xiao,CHEN Yangyang,SHI Xinjie,et al.Effects of spraying exogenous hormones on cuticular wax composition, structure and permeability of the leaves in ‘Yuluxiang’ pear[J].Journal of Nanjing Agricultural University,2018,41(4):647-654.[doi:10.7685/jnau.201710007]
点击复制

喷施外源激素对‘玉露香’梨叶片表皮蜡质组分、结构及渗透性的影响()
分享到：

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年4期
页码:
647-654
栏目:
出版日期:
2018-07-09

文章信息/Info

Title:
Effects of spraying exogenous hormones on cuticular wax composition, structure and permeability of the leaves in ‘Yuluxiang’ pear
作者:
吴潇;  陈杨杨;  石新杰;  齐开杰;  曹鹏;  殷豪;  张绍铃
南京农业大学梨工程技术研究中心, 江苏 南京 210095
Author(s):
WU Xiao;  CHEN Yangyang;  SHI Xinjie;  QI Kaijie;  CAO Peng;  YIN Hao;  ZHANG Shaoling
Pear Engineering and Technology Research Center, Nanjing Agricultural University, Nanjing 210095, China
关键词:
梨; 氨基乙酰丙酸; 茉莉酸甲酯; 水杨酸; 表皮蜡质
Keywords:
pear; aminolevulinic acid(ALA); methyl jasmonate(MeJA); salicylic acid(SA); cuticular wax
分类号:
S661.2
DOI:
10.7685/jnau.201710007
摘要:
[目的]本文旨在揭示外源激素氨基乙酰丙酸（ALA）、茉莉酸甲酯（MeJA）和水杨酸（SA）对植物叶片表皮蜡质合成的影响。[方法]以‘玉露香’梨为试材，叶片喷施不同浓度的ALA、MeJA、SA及MeJA+SA，研究其对叶片表皮蜡质含量、组分、结构以及渗透性的影响。[结果]喷施外源激素后，与对照相比，ALA和MeJA处理的叶片蜡质总量均增加，SA处理的差异不大，而MeJA+SA处理的蜡质总量减少，且不同处理7 d时蜡质总量均高于14 d。喷施不同外源激素后蜡质各组分也发生明显的变化。与对照相比，ALA处理叶片酯类含量在7 d时减少而在14 d时增加，伯醇含量在2个时期均增加。MeJA处理叶片烷烃含量在7 d时增加而在14 d时减少。SA处理7 d时叶片蜡质组分影响较小，14 d时伯醇和酯类含量均增加。MeJA+SA处理叶片酯类含量在7 d时减少，而烷烃、脂肪酸含量在14 d时减少。其中，C27、C30、C31烷烃，C26、C28、C30伯醇和C16、C18脂肪酸是被激素诱导的主要蜡质成分。‘玉露香’梨叶片表面为薄膜状蜡质层，表面较平滑，有褶皱，无裂纹。ALA以及MeJA+SA处理后蜡质层褶皱减少，而MeJA处理后叶片表面褶皱明显增多、变密，且出现较多蜡质晶体。SA处理对蜡质结构影响较小。此外，ALA、MeJA和SA处理能够减缓叶片的水分散失，而MeJA+SA处理加快叶片的水分散失。[结论]本研究表明蜡质层的沉积与结构变化可降低渗透性，减缓叶片的水分散失。SA、MeJA和ALA处理可能通过增加C27以上碳链长度的烷烃含量，减少蜡质的失水率。
Abstract:
[Objectives]The aim of this research is to reveal the regulation effect of aminolevulinic acid(ALA),methyl jasmonate(MeJA)and salicylic acid(SA)to the cuticular wax composition and deposition.[Methods]In this study,the leaves of ‘Yuluxiang’ pear were used as materials,treated with different concentration of ALA,MeJA,SA and combination of MeJA and SA,and their effects on the cuticular wax content,composition,structure and permeability were studied.[Results]The total wax content increased after ALA and MeJA treatments,but no obvious change was detected after SA treatment. On the contrast,the total wax content decreased after the combination treatments of MeJA and SA. Moreover,the total wax content highly improved at 7 days than 14 days after all the different treatments. The wax component significantly changed in ‘Yuluxiang’ pear leaf after exterior hormones treatment. The content of esters decreased at 7 days,whereas increased at 14 days,and the content of primary alcohols both increased at 7 and 14 days after ALA treatment. The content of alkane increased at 7 days,whereas decreased at 14 days after MeJA treatment. SA treatment had a little impact on wax component at 7 days,and the content of primary alcohols and esters decreased at 14 days. The esters content decreased after the combination treatments of MeJA and SA at 7 days,and the content of alkanes and fatty acids decreased at 14 days. C27,C30,C31 alkane,C26,C28,C30 primary alcohol and C16,C18 fatty acid compound were the main cuticular wax constituents induced by exterior hormones application. Scan electric microscope analysis indicted that cuticular wax layer on the leaf surface of ‘Yuluxiang’ pear was smooth and folding without visible wax crystals,shaped like typical smooth wax films. The folding structures of cuticular wax on leaf surface were reduced under ALA and combined of MeJA and SA treatments. MeJA application increased the distribution density of folding structures and wax crystalloids. The SA treatment had less influence on leaf wax crystal structure. In addition,ALA,MeJA and SA treatments also reduced the leaf permeability,leading to the leaf water loss delay,whereas the combination treatments of MeJA and SA caused the improvement of the leaf permeability and accelerated the leaf water loss.[Conclusions]The cuticular wax deposition and crystal structure alteration can reduce the leaf permeability and also delay the leaf water loss,which may be caused by the specific increase of n-alkane(more than C27)after ALA,MeJA and SA treatments.

参考文献/References:

[1] Wu X,Yin H,Chen Y Y,et al. Chemical composition,crystal morphology and key gene expression of cuticular waxes of Asian pears at harvest and after storage[J]. Postharvest Biology and Technology,2017,132:71-80.
[2] Teece M A,Zengeya T,Volk T A,et al. Cuticular wax composition of Salix varieties in relation to biomass productivity[J]. Phytochemistry,2008,69(2):396-402.
[3] de Araújo Silva M M,dos Santos D Y A C,Melo-de-Pinna G F A,et al. Chemical composition and ultrastructure of the foliar cuticular wax of two Brazilian cultivars of castor bean(Ricinus communis L.)[J]. Industrial Crops and Products,2017,95:558-563.
[4] Wang Y,Wang J,Chai G,et al. Developmental changes in composition and morphology of cuticular waxes on leaves and spikes of glossy and glaucous wheat(Triticum aestivum L.)[J]. PLoS One,2015,10(10):e0141239.
[5] Wang J Q,Hao H H,Liu R S,et al. Comparative analysis of surface wax in mature fruits between Satsuma mandarin(Citrus unshiu)and ‘Newhall’ navel orange(Citrus sinensis)from the perspective of crystal morphology,chemical composition and key gene expression[J]. Food Chemistry,2014,153:177-185.
[6] Wang W J,Liu X W,Gai X S,et al. Cucumis sativus L. WAX2 plays a pivotal role in wax biosynthesis,influencing pollen fertility and plant biotic and abiotic stress responses[J]. Plant and Cell Physiology,2015,56(7):1339-1354.
[7] Post-Beittenmiller D. Biochemistry and molecular biology of wax production in plants[J]. Annual Review of Plant Biology,1996,47(1):405-430.
[8] 朱双艳,齐军仓,惠宏杉,等. 大麦幼苗叶片表皮蜡质组分及含量对外源ABA的响应及其对表皮透性的影响[J]. 西南农业学报,2015,28(5):1965-1971. Zhu S Y,Qi J C,Hui H S,et al. Responses of epicuticular wax components and contents of barley seedling leaves to exogenous abscisic acid treatment and its impact on epidermal permeability[J]. Southwest China Journal of Agricultural Sciences,2015,25(5):1965-1971(in Chinese with English abstract).
[9] Zhu X,Xiong L. Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax deposition in rice[J]. Proc Natl Acad Sci USA,2013,110(44):17790-17795.
[10] Yu H,Zhang Y,Xie Y,et al. Ethephon improved drought tolerance in maize seedlings by modulating cuticular wax biosynthesis and membrane stability[J]. Journal of Plant Physiology,2017,214:123-133.
[11] Zhang Z,Wei W,Zhu H,et al. W3 is a new wax locus that is essential for biosynthesis of β-diketone,development of glaucousness,and reduction of cuticle permeability in common wheat[J]. PLoS One,2015,10(10):e0140524.
[12] 李帅,赵秋棱,彭阳,等. SA、MeJA和ACC处理对甘蓝型油菜叶角质层蜡质组分、结构及渗透性的影响[J]. 作物学报,2016,42(12):1827-1833. Li S,Zhao Q L,Peng Y,et al. Effects of SA,MeJA,and ACC on leaf cuticular wax constituents,structure and permeability in Brassica napus[J]. Acta Agronomica Sinica,2016,42(12):1827-1833(in Chinese with English abstract).
[13] Cajuste J F,González-Candelas L,Veyrat A,et al. Epicuticular wax content and morphology as related to ethylene and storage performance of ‘Navelate’ orange fruit[J]. Postharvest Biology and Technology,2010,55(1):29-35.
[14] 马巧利. 2,4-二氯苯氧乙酸保鲜柑橘果实采后品质机理[D]. 武汉:华中农业大学,2014. Ma Q L. Mechanism underlying 2,4-dichlorophenoxyacetic acid maintain citrus fruit postharvest quality[D]. Wuhan:Huazhong Agricultural University,2014(in Chinese with English abstract).
[15] 唐瑛,王文娟,李永才,等. 采后乙烯利、1-MCP处理对苹果梨蜡质组分的影响[J]. 食品科学,2016,37(18):223-229. Tang Y,Wang W J,Li Y C,et al. Effect of postharvest ethephon and 1-MCP ereatments on the chemical composition of cuticular wax in Pingguoli pear fruit[J]. Food Science,2016,37(18):223-229(in Chinese with English abstract).
[16] Li Y C,Yin Y,Chen S J,et al. Chemical composition of cuticular waxes during fruit development of Pingguoli pear and their potential role on early events of Alternaria alternata infection[J]. Functional Plant Biology,2014,41:313-320.
[17] 徐静,王美玲,李婷婷,等. 烟草叶片表面蜡质成分及含量研究[J]. 西北农业学报,2014,23(4):140-145. Xu J,Wang M L,Li T T,et al. Composition and content of tobacco(Nicotiana tabacum L.)leaf cuticular waxes[J]. Acta Agriculturae Boreali-Occidentalis Sinica,2014,23(4):140-145(in Chinese with English abstract).
[18] Jetter R,Schäffer S. Chemical composition of the Prunus laurocerasus leaf surface. Dynamic changes of the epicuticular wax film during leaf development[J]. Plant Physiology,2001,126(4):1725-1737.
[19] 张海禄,齐军仓,韦鹏飞. 大麦叶片表皮蜡质含量的动态变化[J]. 西北农业学报,2013,22(4):49-53. Zhang H L,Qi J C,Wei P F. Dynamics of epidermal wax content of leaves in barley[J]. Acta Agriculturae Boreali-Occidentalis Sinica,2013,22(4):49-53(in Chinese with English abstract).
[20] Zhang J Y,Broeckling C D,Sumner L W,et al. Heterologous expression of two Medicago truncatula putative ERF transcription factor genes,WXP1 and WXP2,in Arabidopsis led to increased leaf wax accumulation and improved drought tolerance,but differential response in freezing tolerance[J]. Plant Molecular Biology,2007,64(3):265-278.
[21] Bourdenx B,Bernard A,Domergue F,et al. Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses[J]. Plant Physiology,2011,156:29-45.
[22] Niki T,Mitsuhara I,Seo S,et al. Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related(PR)protein genes in wounded mature tobacco leaves[J]. Plant and Cell Physiology,1998,39(5):500-507.
[23] Baker E A,Procopiou J,Hunt G M. The cuticles of Citrus species. Composition of leaf and fruit waxes[J]. Journal of the Science of Food and Agriculture,1975,26(8):1093-1101.
[24] Chu W J,Gao H Y,Cao S F,et al. Composition and morphology of cuticular wax in blueberry(Vaccinium spp.)fruits[J]. Food Chemistry,2017,219:436-442.

相似文献/References:

[1]申春苗,汪良驹,王文辉,等.12个梨品种果实冰点温度的检测与影响因素分析[J].南京农业大学学报,2011,34(1):35.[doi:10.7685/j.issn.1000-2030.2011.01.007]
　SHEN Chun-miao,WANG Liang-ju,WANG Wen-hui,et al.Determination of the freezing point temperature of 12 pear cultivars and the correlation analysis of the impact factors[J].Journal of Nanjing Agricultural University,2011,34(4):35.[doi:10.7685/j.issn.1000-2030.2011.01.007]
[2]路娟,吴俊,张绍铃,等.不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011,34(2):38.[doi:10.7685/j.issn.1000-2030.2011.02.008]
　LU Juan,WU Jun,ZHANG Shao-ling,et al.Genetic diversity and polygentic relationship among pears reveled by SSR analysis[J].Journal of Nanjing Agricultural University,2011,34(4):38.[doi:10.7685/j.issn.1000-2030.2011.02.008]
[3]陈迪新,张绍铃,陶书田.沙梨花粉原位萌发与花粉管生长特性[J].南京农业大学学报,2004,27(3):34.[doi:10.7685/j.issn.1000-2030.2004.03.008]
　CHEN Di xin,ZHANG Shao ling,TAO Shu tian.Characteristics of pollen germination and pollen tube growth in in vivo sand pear styles[J].Journal of Nanjing Agricultural University,2004,27(4):34.[doi:10.7685/j.issn.1000-2030.2004.03.008]
[4]沙守峰,李俊才,张绍铃,等.‘新苹梨’果实和叶片发育期有机酸含量的变化及其相关性研究[J].南京农业大学学报,2011,34(6):41.[doi:10.7685/j.issn.1000-2030.2011.06.008]
　SHA Shou-feng,LI Jun-cai,ZHANG Shao-ling,et al.Correlation analysis of changes in the contents of organic acid in developing fruit and leaf of ‘Xinping pear’[J].Journal of Nanjing Agricultural University,2011,34(4):41.[doi:10.7685/j.issn.1000-2030.2011.06.008]
[5]王鑫,吴华清,陶书田,等.大棚和露地栽培条件下梨果实发育特性的差异[J].南京农业大学学报,2012,35(2):27.[doi:10.7685/j.issn.1000-2030.2012.02.005]
　WANG Xin,WU Hua-qing,TAO Shu-tian,et al.Difference of pear fruit development feature between plastic tunnel and open field[J].Journal of Nanjing Agricultural University,2012,35(4):27.[doi:10.7685/j.issn.1000-2030.2012.02.005]
[6]申明,成学慧,谢荔,等.氨基酸叶面肥对砂梨叶片光合作用的促进效应[J].南京农业大学学报,2012,35(2):81.[doi:10.7685/j.issn.1000-2030.2012.02.014]
　SHEN Ming,CHENG Xue-hui,XIE Li,et al.Effects of amino-acid fertilizers on photosynthesis in leaves of pear cultivars[J].Journal of Nanjing Agricultural University,2012,35(4):81.[doi:10.7685/j.issn.1000-2030.2012.02.014]
[7]宋晓晖,谢凯,李艳丽,等.HPLC-ELSD法测定梨果实中不同种类可溶性糖含量[J].南京农业大学学报,2012,35(2):87.[doi:10.7685/j.issn.1000-2030.2012.02.015]
　SONG Xiao-hui,XIE Kai,LI Yan-li,et al.Determination of contents and components of water-soluble sugar in pear fruit by HPLC-ELSD[J].Journal of Nanjing Agricultural University,2012,35(4):87.[doi:10.7685/j.issn.1000-2030.2012.02.015]
[8]陈慧,张树军,张妤艳,等.40个梨品种S基因型的鉴定及S基因频率分析[J].南京农业大学学报,2013,36(5):21.[doi:10.7685/j.issn.1000-2030.2013.05.004]
　CHEN Hui,ZHANG Shujun,ZHANG Yuyan,et al.Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus[J].Journal of Nanjing Agricultural University,2013,36(4):21.[doi:10.7685/j.issn.1000-2030.2013.05.004]
[9]李芳芳,张绍铃,张虎平,等.不同梨品种花粉量及花粉萌发率差异研究[J].南京农业大学学报,2013,36(5):27.[doi:10.7685/j.issn.1000-2030.2013.05.005]
　LI Fangfang,ZHANG Shaoling,ZHANG Huping,et al.Cluster analysis for the quantity and germinating characteristics of the pollens from different pear cultivars[J].Journal of Nanjing Agricultural University,2013,36(4):27.[doi:10.7685/j.issn.1000-2030.2013.05.005]
[10]王超,张全军,黄小三,等.‘金坠’梨成花相关基因PbLFY、PbTFL1和PbFT的克隆及时空表达模式分析[J].南京农业大学学报,2014,37(2):26.[doi:10.7685/j.issn.1000-2030.2014.02.005]
　WANG Chao,ZHANG Quanjun,HUANG Xiaosan,et al.Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear[J].Journal of Nanjing Agricultural University,2014,37(4):26.[doi:10.7685/j.issn.1000-2030.2014.02.005]

备注/Memo

备注/Memo:
收稿日期:2017-10-05。
基金项目:国家自然科学基金项目（31701890）；中央高校基本科研业务费专项资金（KJQN201818）；江苏省自然科学基金项目（BK20160715）
作者简介:吴潇,博士研究生。
通信作者:殷豪,讲师,主要从事梨果实品质分子生物学的研究,E-mail:yinhao@njau.edu.cn
更新日期/Last Update: 1900-01-01