[1]李静,操一凡,丁佳兴,等.含复合菌群生物育苗基质的研制及其育苗效果[J].南京农业大学学报,2018,41(4):676-684.[doi:10.7685/jnau.201801009]
 LI Jing,CAO Yifan,DING Jiaxing,et al.Development of bio-nursery substrates containing PGPR flora and evaluation of their seedlings growth promoting effect[J].Journal of Nanjing Agricultural University,2018,41(4):676-684.[doi:10.7685/jnau.201801009]
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含复合菌群生物育苗基质的研制及其育苗效果()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年4期
页码:
676-684
栏目:
出版日期:
2018-07-09

文章信息/Info

Title:
Development of bio-nursery substrates containing PGPR flora and evaluation of their seedlings growth promoting effect
作者:
李静 操一凡 丁佳兴 孙玉菡 郑宇 胡官墨 沈宗专 李荣 沈其荣
南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化高技术研究重点实验室/国家有机类肥料工程技术研究中心/江苏省有机固体废弃物资源化协同创新中心, 江苏 南京 210095
Author(s):
LI Jing CAO Yifan DING Jiaxing SUN Yuhan ZHENG Yu HU Guanmo SHEN Zongzhuan LI Rong SHEN Qirong
College of Resources and Environmental Sciences/Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization/National Engineering Research Center for Organic-based Fertilizers/Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing 210095, China
关键词:
根际促生菌菌株促生生物育苗基质溶磷菌产生长素菌
Keywords:
PGPR strainsplant growth promotionbio-nursery substratesphosphorus solubilizing bacteriaIAA-production bacteria
分类号:
S144.9
DOI:
10.7685/jnau.201801009
摘要:
[目的]开发含复合菌群生物制品或农业投入品,对于促进作物增产具有重要意义,本文旨在利用分别具有拮抗病原菌、解磷、产生长素能力的菌株,研制含根际促生菌菌群的生物育苗基质,以期为育苗基质产业的发展提供技术支撑。[方法]以本实验室经过广泛田间试验验证,且具有促生及抗土传病害功能的解淀粉芽胞杆菌SQR9为核心菌株,筛选出与之无拮抗效应的产生长素和具解磷能力的菌株;采用Biolog全自动微生物鉴定系统测定菌株对不同碳源利用能力,通过碳源重合关系确定菌种组合;通过育苗和盆栽试验评估含复合菌群生物育苗基质的育苗效果。[结果]经过筛选,与菌株SQR9对峙无拮抗圈的溶磷菌株分别为Y40、Y2和NJAU-3;产吲哚乙酸(IAA)菌株分别为NJAU-5、L-60、NJAU-69和NJAU-84。利用碳源重合关系,构建4种菌株组合2个、3种菌株组合6个和2种菌株组合4个。2季苗盘试验及2季盆栽试验结果均表明,微生物碳源利用重合数与植物促生效果呈显著负相关关系,含菌株SQR9、Y2和L-60与含菌株SQR9、Y2和NJAU-84这2个含复合菌群功能型生物育苗基质,在黄瓜前期育苗效果和后期盆栽效果均优于单菌处理及不添加功能菌普通育苗基质(对照)。[结论]结合拮抗效应测定和碳源利用重合关系,能够有效筛选出高效菌群组合,最终确立含3种菌株的2组复合菌群SQR9+Y2+L-60和SQR9+Y2+NJAU-84为研制生物育苗基质的最佳组合。
Abstract:
[Objectives]The development of biocomposite products or agricultural inputs containing compound bacteria is of great significance for the promotion of crop yield. In this paper,we aimed to develop bio-nursery substrates with plant growth promoting rhizobacteria(PGPR)flora by using bacterial strains with antagonistic ability against pathogen,phosphate-solubilizing ability or auxin-producing capability for providing theoretical and technical support for the development of seedling nursery substrate industry.[Methods]Bacillus amyloliquefaciens SQR9,having been demonstrated to efficiently promote plant growth and suppress the soil-borne diseases,was selected as the core,and antagonistic results of different strains with strain SQR9 were first evaluated to selecte the coexistence strains. Then,Biolog Gen Ⅲ plate and automatic microbial identification system were used to evaluate the different carbon sources utilization abilities for strains,and overlapping relationship of arbon sources utilization among different strains were adopted to design the PGPR flora. At last,seedling nursing and pot experiments were performed to determin the plant growth promotion ability of the bio-nursery substrates produced by different PGPR flora.[Results]After screening,strains without antagonistic phenomen to SQR9 were Y40,Y2 and NJAU-3 with phosphate-solubilizing ability,and NJAU-5,L-60,NJAU-69 and NJAU-84 with auxin-producing ability. Via the carbon source overlap relationship,2 combinations of 4 strains,6 combinations of 3 strains and 4 combinations of 2 strains were established. The results of two-season seedling nursing and pot experiments showed that there was a significant negative correlationship between the utilization overlap of microbial carbon sources and the plant growth promoting effect. Seedling raising experiments showed that in the substrate inoculated with strains SQR9,Y2 and L-60 and strains SQR9,Y2 and NJAU-84,cucumber seedlings grew much better than those in the ordinary nursery substrate and other bio-nursery substrates. The pot experiments showed that the plants growing in the bio-nursery substrate were significantly higher than those in the ordinary nursery substrate and other bio-nursery substrates.[Conclusions]High efficient bacterial group can be effectively selected by combining the antagonistic effect determination and evaluation of overlapping relationship of arbon sources utilization. In this study,the two groups of bacteria with 3 strains(SQR9+Y2+L-60,SQR9+Y2+NJAU-84)were finally selected for the development of the best bio-nursery substrates.

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备注/Memo

备注/Memo:
收稿日期:2018-01-08。
基金项目:国家重点研发计划项目(2016YFE0101100,2016YFD0800605);江苏省科技计划项目(BY2016077-05);江苏高校品牌专业建设工程资助项目(PPZY2015A061);国家大学生创新训练计划项目(201610307004)
作者简介:李静,硕士研究生。
通信作者:李荣,副教授,博士,从事土壤微生物分子生态学研究,E-mail:lirong@njau.edu.cn
更新日期/Last Update: 1900-01-01