[1]田时祎,王珏,汪晶,等.早期低聚半乳糖干预对哺乳仔猪回肠形态、功能发育相关基因及回肠菌群的影响[J].南京农业大学学报,2018,41(5):917-924.[doi:10.7685/jnau.201711031]
 TIAN Shiyi,WANG Jue,WANG Jing,et al.The effect of early intervention with galacto-oligosaccharides on the morphological structure,functional development, and microbial community in ileum of suckling piglets[J].Journal of Nanjing Agricultural University,2018,41(5):917-924.[doi:10.7685/jnau.201711031]
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早期低聚半乳糖干预对哺乳仔猪回肠形态、功能发育相关基因及回肠菌群的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年5期
页码:
917-924
栏目:
出版日期:
2018-09-20

文章信息/Info

Title:
The effect of early intervention with galacto-oligosaccharides on the morphological structure,functional development, and microbial community in ileum of suckling piglets
作者:
田时祎 王珏 汪晶 朱伟云
南京农业大学动物科技学院/国家动物消化道营养国际联合研究中心, 江苏 南京 210095
Author(s):
TIAN Shiyi WANG Jue WANG Jing ZHU Weiyun
College of Animal Science and Technology/National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095 China
关键词:
低聚半乳糖哺乳仔猪回肠菌群组成形态结构功能
Keywords:
galacto-oligosaccharidessuckling pigletsileummicrobial communitymorphological structurefunction
分类号:
S828.8
DOI:
10.7685/jnau.201711031
摘要:
[目的]本文旨在探讨早期低聚半乳糖干预对哺乳仔猪回肠形态结构、功能发育相关基因及回肠菌群的影响。[方法]将6窝(每窝10头)新生仔猪,每窝分2组,即每窝5头分别为对照组、试验组,2组仔猪平均体质量相同。出生后1~7 d,试验组仔猪每天分2次灌喂低聚半乳糖水溶液,低聚半乳糖的有效灌喂量为1 g·kg-1·d-1,对照组仔猪灌喂相同剂量的生理盐水,观察至21日龄断奶。[结果]在8日龄时与对照组仔猪相比,早期低聚半乳糖干预显著提高8日龄回肠黏膜中IGF-1R、VEGFA、Muc2β-defensin基因的相对表达量,并显著提高回肠食糜中总菌和乳酸杆菌的数量。在21日龄,与对照组仔猪相比,早期低聚半乳糖干预显著提高IGF-1β-defensin基因的相对表达量以及屏障蛋白Occludin的相对表达量,显著提高了试验组仔猪回肠的绒毛隐窝比、蔗糖酶活性,显著增加回肠食糜中乳酸杆菌的数量,并显著降低隐窝中的增殖细胞核抗原阳性细胞数。[结论]早期低聚半乳糖干预可改善回肠肠道形态结构,提高回肠对碳水化合物的消化与吸收能力,促进回肠肠道发育,调节回肠肠道菌群组成,并在一定程度上提高回肠肠道屏障功能。
Abstract:
[Objectives]The aim of this study was to investigate the effects of early intervention with galacto-oligosaccharides(GOS) on the morphological structure,functional development,and microbial community in ileum of suckling piglets.[Methods]A total of 6 litters(10 piglets per litter) neonatal piglets(Duroc×Landrace×Large White) were used in the investigation. 5 piglets in each litter were orally administrated with GOS solution twice a day at 1 g·kg-1·d-1 GOS level from the age of 1 to 7 days(referred as GOS group) while the other 5 piglets were treated with same dose of physiological saline(referred as CON group). The average weight of piglets in both groups was the same in the beginning of the experiment.[Results]On 8 day old,compared with CON group,GOS intervention significantly increased the mRNA expression of IGF-1R,VEGFA,Muc2 and β-defensin in ileal mucosa,and significantly increased the quantity of total bacteria and Lactobacillus in ileal digesta. On 21 day old,compared with CON group,GOS intervention significantly increased the mRNA expression of IGF-1 and β-defensin,and the protein expression of Occludin in ileal mucosa. In addition,the results showed that the ratio of villus height:crypt depth was significantly improved. On the other hand,sucrase activity in ileal mucosa was up-regulated,and the proliferating cell nuclear antigen expression in crypt decreased. Furthermore,the quantity of Lactobacillus in ileal digesta was increased.[Conclusions]Early intervention with galacto-oligosaccharides could improve the ileal morphology,enhance the ileal digestive and absorptive ability of carbohydrates,promote the ileal development,regulate the composition of ileal microflora,and improve the ileal barrier function of piglets.

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备注/Memo

备注/Memo:
收稿日期:2017-11-21。
基金项目:国家重点研发计划项目(2017YFD0500505);中央高校基本科研业务费专项资金(KYZ201722)
作者简介:田时祎,硕士研究生。
通信作者:汪晶,教授,博导,主要从事单胃动物营养研究,E-mail:jwang8@njau.edu.cn。
更新日期/Last Update: 1900-01-01