WANG Ying,ZHANG Changwei,LÜ,et al.Genome-wide identification and expression analysis of BrCNGC in Chinese cabbage[J].Journal of Nanjing Agricultural University,2018,41(6):994-1002.[doi:10.7685/jnau.201801037]





Genome-wide identification and expression analysis of BrCNGC in Chinese cabbage
汪影 张昌伟 吕善武 侯喜林
南京农业大学作物遗传与种质创新国家重点实验室/农业农村部华东地区 园艺作物生物学与种质创新重点实验室, 江苏 南京 210095
WANG Ying ZHANG Changwei LÜ Shanwu HOU Xilin
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China
Chinese cabbagecyclic nucleotide gated ion channel gene(CNGC)genome-wide analysisTurnip mosaic virus(TuMV)quantitative real-time PCR
[Objectives] The aim of this study is to investigate the evolutionary relationships, structural features, chromosomal location of the cyclic nucleotide gated ion channel genes in Chinese cabbage (BrCNGC)and to analyze their expression parterns under different treatments. [Methods] The bioinformatics techniques were used to analyze the molecular evolution, genes structure, chromosomal location and conserved motifs of BrCNGC in Chinese cabbage. The expression level of BrCNGC was analyzed by quantitative RT-PCR under different treatments:abscisic acid (ABA)+Turnip mosaic virus (TuMV), salicylic acid (SA)+TuMV, methyl jasmonate (MeJA)+TuMV, and ascorbic acid (AsA)+TuMV. [Results] The results showed that there are 26 BrCNGC genes in Chinese cabbage and can be classified into four major groups (Ⅰ, Ⅱ, Ⅲ, Ⅳ)and two subgroups (Ⅳa and Ⅳb), they were predicted to be located on 9 chromosomes, the chromosome 1 contained five BrCNGC, while the chromosome 5 only contained one BrCNGC and no BrCNGC on chromosome 8. Gene structure analysis showed that 10 kinds of motifs were identified in these genes, 14 BrCNGC proteins contained all of 10 motifs, and the genes that came from the same group shared similar motif composition. The results of quantitative RT-PCR showed that most BrCNGC genes up-regulated and few BrCNGC genes down-regulated by the interaction between plant growth regulator and TuMV. [Conclusions] The structure of BrCNGC in Chinese cabbage is highly conserved and they play a role in the protection against TuMV infection. This finding laid the foundation for the further study on the function of BrCNGC genes.


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更新日期/Last Update: 2018-11-23