[1]张伟,张琳,李茹,等.不同遗传类群抗五氟磺草胺稗种子萌发对逆境条件的响应[J].南京农业大学学报,2018,41(6):1045-1053.[doi:10.7685/jnau.201712005]
 ZHANG Wei,ZHANG Lin,LI Ru,et al.The response of seed germination of penoxsulam-resistant Echinochloa crusgalli(L.)P. Beauv from different genetic backgrounds to adverse conditions[J].Journal of Nanjing Agricultural University,2018,41(6):1045-1053.[doi:10.7685/jnau.201712005]
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不同遗传类群抗五氟磺草胺稗种子萌发对逆境条件的响应()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年6期
页码:
1045-1053
栏目:
出版日期:
2018-11-25

文章信息/Info

Title:
The response of seed germination of penoxsulam-resistant Echinochloa crusgalli(L.)P. Beauv from different genetic backgrounds to adverse conditions
作者:
张伟1 张琳2 李茹3 陈国奇1 董立尧1
1. 南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095;
2. 上海青浦区农业技术推广服务中心, 上海 201700;
3. 徐淮地区淮阴农业科学研究院, 江苏 淮安 223001
Author(s):
ZHANG Wei1 ZHANG Lin2 LI Ru3 CHEN Guoqi1 DONG Liyao1
1. College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China;
2. Shanghai Qingpu District Agricultural Technology Extension Service Center, Shanghai 201700, China;
3. Xuhuai District Huaiyin Academy of Agricultural Sciences, Huai’an 223001, China
关键词:
种子萌发环境因子五氟磺草胺
Keywords:
Echinochloa crusgalli(L.)P. Beauvseed germinationenvironmental factorspenoxsulan
分类号:
S451
DOI:
10.7685/jnau.201712005
文献标志码:
A
摘要:
[目的]本文旨在明确华东地区隶属苏南、苏北、皖南3个不同遗传类群抗五氟磺草胺稗[Echinochloa crusgalli(L.)P.Beauv]在不同条件下的萌发差异性,确定各遗传类群抗五氟磺草胺稗对不同逆境适应性的差异,为延缓抗性稗的扩散和治理提供理论依据。[方法]采用培养皿法和盆钵法进行种子萌发和出苗试验,测定萌发率并计算发芽势和发芽指数。[结果]当昼/夜温度为10℃/5℃时,敏感种群JLGY-3的萌发率(29.38%)和发芽指数(0.47)显著高于同一遗传背景的抗性种群JHHZ-1,两者的发芽势无显著性差异;当昼/夜温度为40℃/35℃时,两者的各指标均无显著性差异;当NaCl浓度为400 mmol·L-1时,JLGY-3少数萌发,JHHZ-1无萌发,两者差异显著;当水势为-0.6 MPa时,JLGY-3的各指标均显著高于JHHZ-1;在各pH值下或埋土深度为8 cm时,两种群的各指标均无显著性差异。不同遗传背景的抗性种群在昼/夜温度为40℃/35℃时,AXXZ-8的各指标均最低,JHHZ-1的发芽指数(11.58)最高,JHHZ-1与SSXB-1萌发率和发芽势无显著差异,并显著高于AXXZ-8,昼/夜温度为10℃/5℃下的JHHZ-1发芽指数(0.39)显著高于SSXB-1和AXXZ-8,3种群的萌发率和发芽势无显著差异;350 mmol·L-1的NaCl溶液中3种群的各指标均差异显著,并表现为JHHZ-1、SSXB-1、AXXZ-8依次降低;-0.6 MPa水势下AXXZ-8的各指标均最低,JHHZ-1发芽势(17.50)最高,JHHZ-1与SSXB-1的萌发率和发芽指数无显著差异,并显著高于AXXZ-8;当pH值为3或12时,AXXZ-8的发芽指数均最低,JHHZ-1最高,3种群的萌发率和发芽势均表现为JHHZ-1与SSXB-1无显著差异,并显著高于AXXZ-8;埋土深度8 cm的AXXZ-8各指标均最低,JHHZ-1的各指标与SSXB-1无显著差异,并显著高于AXXZ-8。[结论]在种子萌发阶段,抗性稗种群对环境的适应性跟抗性水平呈负相关性,表现出一定程度上的适合度代价。
Abstract:
[Objectives] The objective of this study is to determine the germination differences of barnyardgrass [Echinochloa crusgalli (L.)P. Beauv]from southern Jiangsu, northern Jiangsu and southern Anhui which blong to three different genetic groups. To determine the difference of diffusion ability of penoxsulam-resistant from different genetic groups, this test provides a theoretical basis for delaying the spread and resistance control. [Methods] Petri dish method and pot method were carried out to test the seed germination. And the germination rate, germination energy and germination index were acquired. [Results] The germination rate (29.38%)and the germination index (0.47)of JLGY-3 were significantly higher than those of the resistant population JHHZ-1 from the same genetic background when diurnal temperature was 10℃/5℃, and there was no significant difference in germination potential between two populations. When diurnal temperature was 40℃/35℃, there were no significant differences in three indicators between two groups. JLGY-3 germinated few and JHHZ-1 did not germinate when NaCl concentration was 400 mmol·L-1, and there was significant difference between two populations. The three indicators of JLGY-3 were all significantly higher than those of JHHZ-1 at the water potential of -0.6 MPa. These indicators of two populations showed no significant difference at each pH value or depth of buried soil of 8 cm. The three indicators of AXXZ-8 were the lowest, while the germination index of JHHZ-1 (11.58)was the highest when diurnal temperature was 40℃/35℃. Meanwhile, there was no difference observed between JHHZ-1 and SSXB-1 all of which were them from different genetic backgrounds in the parts of germination energy and germination index, but their two kinds of data were significantly higher than AXXZ-8. When diurnal temperature was 10℃/5℃, the germination index of JHHZ-1 (0.39)was significantly higher than SSXB-1 and AXXZ-8. At the same time, the germination rate and germination energy of the three had no significant difference in this kind of treatment. In the NaCl solution of 350 mmol·L-1, three populations’ germination rate, germination energy and germination index were significantly different and which showed JHHZ-1, SSXB-1 and AXXZ-8 decreased in turn. The three indicators of AXXZ-8 were the lowest at -0.6 MPa. Simultaneously, the germination energy of JHHZ-1 (17.50)was the highest. The germination rate and germination index of JHHZ-1 and SSXB-1 were not significantly different but both were significantly higher than AXXZ-8 in this treatment. When pH value was 3 or 12, the germination index of AXXZ-8 was the lowest, and the germination index of JHHZ-1 was the highest. Germination rate and germination energy showed no significant difference between JHHZ-1 and SSXB-1 but both were significantly higher than AXXZ-8. Three indicators of AXXZ-8 were the lowest at the depth of 8 cm. Meanwhile, these indicators showed no significant difference between JHHZ-1 and SSXB-1 but both were significantly higher than AXXZ-8. [Conclusions] At the stage of seed germination, there was a negative correlation between the adaptability of resistant population to environment and the level of resistance, and it showed a certain degree of fitness cost at the stage of germination.

参考文献/References:

[1] 杨小育. 世界性恶性杂草的分布与危害[J]. 世界农业,1992 (4):40-42. Yang X Y. Distribution and harm of worldwide malignant weeds[J]. World Agriculture,1992 (4):40-42 (in Chinese).
[2] 俞欣妍,葛林利,刘丽萍,等. 直播稻田稗草对二氯喹啉酸、氰氟草醋与双草醚除草剂复合抗性的初步研究[J]. 江苏农业学报,2010,26 (6):1438-1440. Yu X Y,Ge L L,Liu L P,et al. Resistance of barnyardgrass (Echinochloa crusgalli)to multiple herbicides in direct-seeded rice[J]. Jiangsu Journal of Agricultural Sciences,2010,26 (6):1438-1440 (in Chinese).
[3] 朱文达. 稗对水稻生长和产量性状的影响及其经济阈值[J]. 植物保护学报,2005,32 (1):81-86. Zhu W D. Influence of barnyardgrass,Echinochloa crusgalli,on the growth and yield of paddy rice and its economic threshold[J]. Acta Phytophylacica Sinica,2005,32 (1):81-86 (in Chinese with English abstract).
[4] 顾林玲. 三唑并嘧啶磺酰胺类除草剂——五氟磺草胺[J]. 现代农药,2015,14 (2):46-51. Gu L L. Penoxsulam:a triazolopyrimidine sulfonamide herbicide[J]. Modern Agrochemicals,2015,14 (2):46-51 (in Chinese with English abstract).
[5] Heap I. The international survey of herbicide resistant weeds[EB/OL].[2017-12-03]. http://www.weedscience.org/Summary/ResistbyActive.aspx.
[6] 刘兴林,孙涛,付声姣,等. 水稻田除草剂的应用及杂草抗药性现状[J]. 西北农林科技大学学报 (自然科学版),2015,43 (7):115-126. Liu X L,Sun T,Fu S J,et al. Herbicide application and weeds resistance in rice field in China[J]. Journal of Northwest A&F University (Natural Science Edition),2015,43 (7):115-126 (in Chinese with English abstract).
[7] Heap I. Global perspective of herbicide-resistant weeds[J]. Pest Management Science,2014,70:1306-1315.
[8] Chen G Q,Wang Q,Yao Z W. Penoxsulam-resistant barnyardgrass (Echinochloa crusgalli)in rice fields in China[J]. Weed Biology and Management,2016,16 (1):16-23.
[9] Matzenbacher F O,Bortoly E D,Kalsing A,et al. Distribution and analysis of the mechanisms of resistance of barnyardgrass (Echinochloa crus-galli)to imidazolinone and quinclorac herbicides[J]. Journal of Agricultural Science,2015,153 (6):1044-1058.
[10] Beckie H J,Harker K N. Our top 10 herbicide-resistant weed management practices[J]. Pest Management Science,2017,73:1045-1052.
[11] 黄江宇,田家顺,严清平. 水稻水直播稻田抗药性稗草治理研究[J]. 湖南农业科学,2017 (8):64-66. Huang J Y,Tian J S,Yan Q P. Drug resistance barnyardgrass management research of direct-seed rice filed[J]. Hunan Agricultural Sciences,2017 (8):64-66 (in Chinese with English abstract).
[12] 李善林,倪汉文,张丽. 耕翻对稗草、马唐、苋菜三种杂草的防治机理研究[J]. 中国农业大学学报,1998,3 (增刊):65-70. Li S L,Ni H W,Zhang L. Effects of ploughing on controlling redroot,amaranth and common crabgrass baryardgrass[J]. Journal of China Agricultural University,1998,3 (Suppl):65-70 (in Chinese with English abstract).
[13] 王桔红,杜国祯,崔现亮,等. 青藏高原东缘61种常见木本植物种子萌发特性及其与生活史的关联[J]. 植物生态学报,2009,33 (1):171-179. Wang J H,Du G Z,Cui X L,et al. Germination characteristics of 61 common woody species from the eastern QingHai-Tibet plateau of China and their life history correlates[J]. Chinese Journal of Plant Ecology,2009,33 (1):171-179 (in Chinese with English abstract).
[14] Chauhan B S,Johnson D E. Influence of environmental factors on seed germination and seedling emergence of eclipta (Eclipta prostrata)in a tropical environment[J]. Weed Science,2008,56 (3):383-388.
[15] Rao N,Dong L Y,Li J,et al. Influence of environmental factors on seed germination and seedling emergence of American sloughgrass (Beckmannia syzigachne)[J]. Weed Science,2008,56 (4):529-533.
[16] Ebrahimi E,Eslami S V. Effect of environmental factors on seed germination and seedling emergence of invasive Ceratocarpus arenarius[J]. Weed Research,2012,52 (1):50-59.
[17] Chauhan B S,Johnson D E. Germination ecology of southern crabgrass (Digitaria ciliaris)and India crabgrass (Digitaria longiflora):two important weeds of rice in tropics[J]. Weed Science,2008,56 (5):722-728.
[18] Lim C A A,Awan T H,Sta. Cruz P C,et al. Influence of environmental factors,cultural practices,and herbicide application on seed germination and emergence ecology of Ischaemum rugosum Salisb[J]. PLoS One,2015,10 (9):e0137256.
[19] Chauhan B S,Johnson D E. Germination ecology of goosegrass (Eleusine indica):an important grass weed of rainfed rice[J]. Weed Science,2008,56 (5):699-706.
[20] Chauhan B S,Johnson D E. Ecological studies on Echinochloa crusgalli and the implications for weed management in direct-seeded rice[J]. Crop Protection,2011,30 (11):1385-1391.
[21] Chen G Q,Zhang W,Fang J P. Restriction site-associated DNA sequencing allows for the rapid identification of simple sequence repeat markers in Echinochloa crusgalli[J]. Weed Biology and Management,2017,17 (2):68-76.
[22] Michel B E,Kaufmann M R. The osmotic potential of polyethylene glycol 6000[J]. Plant Physiology,1973,51 (5):914-916.
[23] Javaid M M,Tanveer A. Germination ecology of Emex spinosa and Emex australis,invasive weeds of winter crops[J]. Weed Research,2014,54 (6):565-575.
[24] Tang W,Xu X Y,Shen G Q,et al. Effect of environmental factors on germination and emergence of aryloxyphenoxy propanoate herbicide-resistant and-susceptible Asia minor bluegrass (Polypogon fugax)[J]. Weed Science,2015,63 (3):669-675.
[25] Vila-Aiub M M,Neve P,Steadman K J,et al. Ecological fitness of a multiple herbicide-resistant Lolium rigidum population:dynamics of seed germination and seedling emergence of resistant and susceptible phenotypes[J]. Journal of Applied Ecology,2005,42 (2):288-298.
[26] Wu X B,Zhang T,Pan L,et al. Germination requirements differ between fenoxaprop-P-ethyl resistant and susceptible Japanese foxtail (Alopecurus japonicus)biotypes[J]. Weed Science,2016,64 (4):653-663.
[27] Martins J F,Barroso A A M,Alves P L C A. Effects of environmental factors on seed germination and emergence of glyphosate resistant and susceptible sourgrass[J]. Planta Daninha,2017,35:e017164499.
[28] Dinelli G,Marotti I,Catizone P,et al. Germination ecology of Ambrosia artemisiifolia L. and Ambrosia trifida L. biotypes suspected of glyphosate resistance[J]. Central European Journal of Biology,2013,8 (3):286-296.
[29] Kovach D A,Widrlechner M P,Brenner D M. Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing[J]. Seed Science and Technology,2010,38 (3):559-571.
[30] 王娅,张希明,李利,等. 四种猪毛菜种子萌发对主要生态因子的响应[J]. 种子,2008,27 (8):58-67. Wang Y,Zhang X M,Li L,et al. Seed on response of germination of four species of Salsola to main ecology factors[J]. Seed,2008,27 (8):58-67 (in Chinese).
[31] 巴逢辰,赵羿. 中国海涂土壤资源[J]. 土壤通报,1997,28 (2):49-51. Ba F C,Zhao Y. The coastal soil resources in China[J]. Chinese Journal of Soil Science,1997,28 (2):49-51 (in Chinese).
[32] 赵晓英,任继周,王彦荣,等,3种锦鸡儿种子萌发对温度和水分的响应[J]. 西北植物学报,2005,25 (2):211-217. Zhao X Y,Ren J Z,Wang Y R,et al. Germination responses to temperature and moisture in seed from three species of Caragana[J]. Acta Botanica Boreali-Occidentalia Sinica,2005,25 (2):211-217 (in Chinese with English abstract).
[33] 吴宪. 棒头草种子生物学、生态适应性及其化学防除技术研究[D]. 南京:南京农业大学,2015:28-29. Wu X. Seed biology,ecologcal adaptability and chemical control of Asia minor bluegrass[D]. Nanjing:Nanjing Agricultural University,2015:28-29 (in Chinese with English abstract).
[34] Baltazar A M. Herbicide-resistant weeds in the Philippines:status and resistance mechanisms[J]. Weed Biology and Management,2017,17:57-67.

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备注/Memo

备注/Memo:
收稿日期:2017-12-04。
基金项目:国家重点研发计划项目(2018YFD0200300)
作者简介:张伟,硕士研究生。
通信作者:董立尧,教授,博导,研究方向为除草剂毒理及抗药性,E-mail:dly@njau.edu.cn。
更新日期/Last Update: 2018-11-23